Quantification of crotamine, a small basic myotoxin, in South American rattlesnake (Crotalus durissus terrificus) venom by enzyme-linked immunosorbent assay with parallel-lines analysis

Oguiura, Nancy; Camargo, M. E.; Silva, A.R.P. da; Horton, Denise S. P. Q.

doi:10.1016/s0041-0101(99)00157-9

Cited by 30 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This agrees with the findings of other authors (7,12,21,(31)(32)(33)(34)(35)(36)(37)(38)42), which assured that ionizing radiation has the property to attenuate venoms toxicity without altering immunological properties.…”

Section: Discussionsupporting

confidence: 92%

“…This method has showed to present the best specificity, sensitivity, fastness, simplicity, and low costs (3,4,6,32,35,39,44). Results showed that the optical density values obtained in the ELISA test for the several dilutions of the native and irradiated venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni did not show statistical difference between the groups studied.…”

Section: Discussionmentioning

confidence: 99%

“…The principal physical agents are: x-ray radiation (19), ultraviolet radiation (46), heat (15), and gamma radiation (25). The latter, according to previous works (1,7,9,12,14,17,21,(31)(32)(33)(34)(35)(36)(37)(38)(41)(42)(43), has proved to be an excellent tool of detoxification, since it is able to reduce toxicity without altering immunogenicity. In addition, this radiation does not add any new substance to the venom.…”

Section: Introductionmentioning

confidence: 93%

See 2 more Smart Citations

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira¹,

Nascimento²,

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated ( 60 Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD 50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60 th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60 Coirradiation. The sera produced with native venom showed neutralizing potency and capacity similar to those of the sera produced with irradiated venom. All antibodies were able to neutralize five LD 50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom, however, necrosis and death occurred in animals inoculated with native venom.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

See 1 more Smart Citation

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira¹,

Nascimento²,

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…The variation in venom composition and pharmacology has been addressed for different snake venoms at several levels (Chipppaux et al, 1991). Studies on understanding the intra-specific variability of venoms are gaining much attention with the intention of production of efficacious therapeutic antivenom and have been studied extensively (Goncalves, 1956;Glenn et al, 1983;Silveira et al, 1990;Yang et al, 1991;Lomonte and Carmona, 1992;Oguiura et al, 2000;Ferquel et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Systemic pathological effects induced by cobra (Naja naja) venom from geographically distinct origins of Indian peninsula

Shashidharamurthy

Mahadeswaraswamy

Ragupathi

et al. 2010

Experimental and Toxicologic Pathology

View full text Add to dashboard Cite

“…Considering the variation of crotamine concentration in rattlesnake venom (Oguiura et al, 2000) and the difference in signal intensities of the crotamine gene in both homologous chromosomes (Rádis-Baptista et al, 2004), we sought to determine the relationship between the crotamine concentration in the venom and the gene copy number. We also sequenced the exon 2, intron 2 and exon 3 of crotamine and crotasin genes of rattlesnakes from different localities in order to investigate their genetic variability and the evolutionary relationships of the sequences.…”

Section: Introductionmentioning

confidence: 99%

Intraspecific variation of the crotamine and crotasin genes in Crotalus durissus rattlesnakes

Oguiura

Collares

Furtado

et al. 2009

Gene

View full text Add to dashboard Cite

a b s t r a c tCrotamine is a small basic myotoxin peptide of Crotalus durissus venom, with β-defensin scafold and variable concentration in individual venoms. The crotamine gene was mapped to the end of chromosome 2 and the signal intensity differed significantly between the two homologues. In contrast to crotamine, the paralogous crotasin gene is scarcely expressed in the venom glands. In this study, we analyzed the crotamine concentrations in the venoms of a total of 23 rattlesnakes from diverse Brazilian localities by ELISA as well as the copy number of both crotamine and crotasin genes by real-time PCR. Crotamine was found to constitute 5-29% of venom proteins varying greatly among individual animals. The crotamine gene exists from 1 to 32 copies per haploid genome, whereas the crotasin gene is present from 1 to 7 copies. Furthermore, we observed that the crotamine concentration and crotamine gene copy number are positively correlated (r 2 = 0.68), implying the variation of crotamine in venom results from the variation of the gene copy number. Sequencing of 50 independent copies of crotamine and crotasin genes from four different rattlesnakes revealed the presence of six crotasin isoforms with a single amino acid difference from the original crotasin sequence, whereas only two additional crotamine isoforms were observed. Taken together, our results suggested that after duplication from a common ancestor gene, crotamine and crotasin may have diverged in such a way that the crotamine gene underwent repetitive duplication to increase its copy number, whereas the crotasin gene diversified its sequence.

show abstract

Quantification of crotamine, a small basic myotoxin, in South American rattlesnake (Crotalus durissus terrificus) venom by enzyme-linked immunosorbent assay with parallel-lines analysis

Cited by 30 publications

References 9 publications

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Systemic pathological effects induced by cobra (Naja naja) venom from geographically distinct origins of Indian peninsula

Intraspecific variation of the crotamine and crotasin genes in Crotalus durissus rattlesnakes

Contact Info

Product

Resources

About