Quantification of compact bone microporosities in the basal and alveolar portions of the human mandible using osteocyte lacunar density and area fraction of vascular canals

Tonar, Zbyněk; Khadang, Ismail; Fiala, Pavel; Nedorost, Lukáš; Kochová, Petra

doi:10.1016/j.aanat.2011.02.001

Cited by 13 publications

(11 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that point counting is a precise method. There are some other published articles that have used this technique for measuring bone area [25][26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 97%

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Esteves

Moraes

Gomes

et al. 2015

Pathology - Research and Practice

View full text Add to dashboard Cite

“…This suggests that point counting is a precise method. There are some other published articles that have used this technique for measuring bone area [25][26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 97%

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Esteves

Moraes

Gomes

et al. 2015

Pathology - Research and Practice

View full text Add to dashboard Cite

“…10 Similarly, just as cranial bone has a greater density both in terms of density per unit of area (areal bone density) 11 or true mineral content than weight-bearing bone sites 12 ; bone density is greater in basal bone compared with alveolar bone. 13,14 These data suggest that there might be common pathways regulating bone cell behaviour in the ulnar and alveolar bone, and importantly, that these are distinct from the common pathways in the calvarial and basal bone.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles of mandible reveal features of both calvarial and ulnar bones in the adult rat

Kingsmill

McKay

Ryan

et al. 2013

Journal of Dentistry

View full text Add to dashboard Cite

“…Toluidine blue can easily differentiate unmineralized osteoid from fully mineralized bone tissue and provides sufficient preservation of morphological details. Basic fuchsine can be used alone 81 [ Fig. 5(c)] for staining microporous bone or can be combined with toluidine blue.…”

Section: Stainingmentioning

confidence: 99%

“…Vascularization of the bone adjacent to the implant and the numerical density of osteocyte lacunae within the bone may be assessed using the stereological point-counting technique reported in the human mandible by Tonar et al 81 The lacunar microporosities (density of osteocytes) may be statistically independent from the vascular microporosities (bone FIG. 6.…”

Section: Histological Quantification Of Other Bone Characteristicsmentioning

confidence: 99%

Evaluating the osseointegration of nanostructured titanium implants in animal models: Current experimental methods and perspectives (Review)

et al. 2016

View full text Add to dashboard Cite

The aim of this paper is to review the experimental methods currently being used to evaluate the osseointegration of nanostructured titanium implants using animal models. The material modifications are linked to the biocompatibility of various types of oral implants, such as laser-treated, acid-etched, plasma-coated, and sand-blasted surface modifications. The types of implants are reviewed according to their implantation site (endoosseous, subperiosteal, and transosseous implants). The animal species and target bones used in experimental implantology are carefully compared in terms of the ratio of compact to spongy bone. The surgical technique in animal experiments is briefly described, and all phases of the histological evaluation of osseointegration are described in detail, including harvesting tissue samples, processing undemineralized ground sections, and qualitative and quantitative histological assessment of the bone-implant interface. The results of histological staining methods used in implantology are illustrated and compared. A standardized and reproducible technique for stereological quantification of bone-implant contact is proposed and demonstrated. In conclusion, histological evaluation of the experimental osseointegration of dental implants requires careful selection of the experimental animals, bones, and implantation sites. It is also advisable to use larger animal models and older animals with a slower growth rate rather than small or growing experimental animals. Bones with a similar ratio of compact to spongy bone, such as the human maxilla and mandible, are preferred. A number of practical recommendations for the experimental procedures, harvesting of samples, tissue processing, and quantitative histological evaluations are provided.

show abstract

Quantification of compact bone microporosities in the basal and alveolar portions of the human mandible using osteocyte lacunar density and area fraction of vascular canals

Cited by 13 publications

References 54 publications

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Gene expression profiles of mandible reveal features of both calvarial and ulnar bones in the adult rat

Evaluating the osseointegration of nanostructured titanium implants in animal models: Current experimental methods and perspectives (Review)

Contact Info

Product

Resources

About