Quantification of Chlorogenic Acid and Hyperoside Directly from Crude Blueberry (Vaccinium angustifolium) Leaf Extract by NMR Spectroscopy Analysis: Single-Laboratory Validation

Hicks, Joshua M; Asim, Muhammad; Ferrier, Jonathan; Saleem, Ammar; Cuerrier, Alain; Arnason, John T.; Colson, Kimberly L.

doi:10.5740/jaoacint.11-415

Cited by 24 publications

(13 citation statements)

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“…Hyperoside is quercetin-3-O-galactoside. It was found out in strawberries [Bagdonaite et al 2013], blueberries [Hicks et al 2012], whitethorn [Belkhir et al 2013] and rowan [Gaivelyte et al 2013]. Among herbs, it is abundant above all in St John's wort [Cirak et al 2013].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Antioxidants by HPLC-MS in Grapevine Seeds

Sochorová

Klejdus

Baroň

et al. 2019

asphc

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It is well known, that grapevine seeds are rich in significant antioxidants. However, the issue of dealing with the analysis and comparison of antioxidant components in the seeds of Vitis vinifera L. in individual cultivars has not yet been sufficiently studied. The experiment was performed with extracts of three varieties (Blaufränkish, Italian Riesling and Cabernet Moravia) and three interspecific cultivars (Nativa, Marlen and Kofranka). Contents of nine major flavonoids (apigenin, astragalin, hyperoside, isorhamnetin, kaempferol, myricetin, quercetin, quercitrin and rutin) and two procyanidins (procyanidin A 2 and procyanidin B 1 ) were assessed by the HPLC/MS method. The highest contents of antioxidants were found out in interspecific cultivars Marlen and Nativa while the lowest one was assessed in the cultivar Cabernet Moravia. The most represented flavonoid was hyperoside (cultivar Marlen -15.66 mg•l -1 ), least represented was kaempferol (cultivar Cabernet Moravia -1.81 µg•l -1 ).

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Section: Resultsmentioning

confidence: 99%

Assessment of Antioxidants by HPLC-MS in Grapevine Seeds

Sochorová

Klejdus

Baroň

et al. 2019

asphc

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“…Chlorogenic acids accumulate in leaves and fruits where they act as feeding deterrents and antifungal agents. They also have potent antioxidant properties which have been shown to protect crops from intense UV radiation damage (Mondolot et al ., ; Cle et al ., ; Hicks et al ., ). Chlorogenic acids are derived through conjugation of quinate and hydroxycinnamoyl moieties (Lallemand et al ., ; Escamilla‐Treviño et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Structural and biochemical approaches uncover multiple evolutionary trajectories of plant quinate dehydrogenases

et al. 2018

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Quinate is produced and used by many plants in the biosynthesis of chlorogenic acids (CGAs). Chlorogenic acids are astringent and serve to deter herbivory. They also function as antifungal agents and have potent antioxidant properties. Quinate is produced at a branch point of shikimate biosynthesis by the enzyme quinate dehydrogenase (QDH). However, little information exists on the identity and biochemical properties of plant QDHs. In this study, we utilized structural and bioinformatics approaches to establish a QDH-specific primary sequence motif. Using this motif, we identified QDHs from diverse plants and confirmed their activity by recombinant protein production and kinetic assays. Through a detailed phylogenetic analysis, we show that plant QDHs arose directly from bifunctional dehydroquinate dehydratase-shikimate dehydrogenases (DHQD-SDHs) through different convergent evolutionary events, illustrated by our findings that eudicot and conifer QDHs arose early in vascular plant evolution whereas Brassicaceae QDHs emerged later. This process of recurrent evolution of QDH is further demonstrated by the fact that this family of proteins independently evolved NAD and NADP specificity in eudicots. The acquisition of QDH activity by these proteins was accompanied by the inactivation or functional evolution of the DHQD domain, as verified by enzyme activity assays and as reflected in the loss of key DHQD active site residues. The implications of QDH activity and evolution are discussed in terms of plant growth and development.

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“…The presence of several major phenolic compounds in the crude leaf extract reported by McIntyre et al (), Hicks et al (), and Ferrier et al () was reconfirmed using UPLC–QTOF–MS. The phenolic compounds reconfirmed in the extract include chlorogenic acid, myricetin, and hyperoside (Table ).…”

Section: Resultsmentioning

confidence: 68%

“…Vaccinium extracts, including those from V. angustifolium, are a rich source of phenolic compounds (Harris et al, 2007;Hicks et al, 2012;Markus et al, 2014;McIntyre et al, 2009) and have been shown to influence cell migration and invasion using in vitro models of cancer metastasis (Adams et al, 2010;Matchett, MacKinnon, Sweeney, Gottschall-Pass, & Hurta, 2005), in vitro models of endothelial cell migration and invasion (Tulio et al, 2012), and animal models of wound healing (Nayak et al, 2010). In this study, the .…”

Section: Discussionmentioning

confidence: 99%

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

Ferrier

Gaudet

et al. 2018

Phytotherapy Research

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Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 10 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p < .01) and did not affect cell proliferation or viability. Chlorogenic acid was identified as a major phenolic compound of the leaf extract and the most active compound. Evidence from Western blot analysis (n = 3) suggests that the effects of the leaf extract and chlorogenic acid on trophoblast migration and invasion are mediated through an adenosine monophosphate-activated protein (AMP) kinase-dependent mechanism. Further investigations examining the potential therapeutic applications of this natural health product extract and its major chemical compounds in the context of placenta-mediated diseases are warranted.

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Quantification of Chlorogenic Acid and Hyperoside Directly from Crude Blueberry (Vaccinium angustifolium) Leaf Extract by NMR Spectroscopy Analysis: Single-Laboratory Validation

Cited by 24 publications

References 0 publications

Assessment of Antioxidants by HPLC-MS in Grapevine Seeds

Assessment of Antioxidants by HPLC-MS in Grapevine Seeds

Structural and biochemical approaches uncover multiple evolutionary trajectories of plant quinate dehydrogenases

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

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