Quantification of cell types throughout the cycle of the human seminiferous epithelium and their DNA content

Paniagua, Ricardo; Codesal, J.; Nistal, Manuel; Rodríguez, Manuel; Santamarı́a, Luis

doi:10.1007/bf00310055

Cited by 32 publications

(21 citation statements)

References 10 publications

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“…Also in the adult seminiferous epithelium, the spermatogonia stem and precursor cells are represented by a specific lineage that shows morphological and topographical differences between rodents (Huckins 1971;Oakberg 1971;Huckins and Oakberg 1978;De Rooij 1973, 1988Lok et al 1982;Van Haaster and De Rooij 1993), primates including man (Chowdhury and Steinberger 1977;W Schulze 1978a, b;C Schulze 1979;Paniagua et al 1987;Feldmeier 1998) and large ungulates viz. bull (Wrobel et al 1995a(Wrobel et al , b, 1996.…”

Section: Discussionmentioning

confidence: 99%

Prespermatogenesis and spermatogoniogenesis in the bovine testis

Wrobel¹

2000

Anatomy and Embryology

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The bovine male germ cell population was studied over the entire period from testicular differentiation in the embryo through onset of spermatogenesis in the pubertal calf. Germ cells were identified by protein gene product 9.5 immunohistochemistry and characterized by their ultrastructure. The proliferation pattern of germ cells was studied with immunohistochemical anti-Ki 67 and anti-proliferating cell nuclear antigen reactions. Germ cells with a high proliferation rate are observed from day 50 p.c. to day 80 p.c. These cells are in transition from primordial germ cells to prespermatogonia. From day 80 p.c. until approximately the 15th postnatal week the germ cells present are identified as prespermatogonia. From day 80 p.c. to day 200 p.c. germ cell multiplication decreases continuously; then the prespermatogonia enter a phase of relative mitotical quiescence that lasts until the 4th postnatal week. Between the 4th and the 15th postnatal week, testicular tubular diameters grow from 40 to 80 microm and the prespermatogonia resume their proliferation. In seminiferous tubules with diameters between 80 and 120 microm, found in animals between 18 and 27 weeks of age, a central lumen is normally still absent. During this period germ cell proliferation reaches a second maximum. The cells involved represent the members of the spermatogonia stem and precursor cell line kinetically interpolated between the prespermatogonia and the first differentiating A-spermatogonia. This second phase of prepubertal germ cell multiplication coincides with the period when the pre-Sertoli cells transform into adult-type Sertoli cells and enter the G0-phase for the rest of life.

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Section: Discussionmentioning

confidence: 99%

Prespermatogenesis and spermatogoniogenesis in the bovine testis

Wrobel¹

2000

Anatomy and Embryology

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“…In the human testis, type-A spermatogonia are also known to represent stem cell populations that maintain cell multiplication by continuous mitotic division (Paniagua et al 1987).…”

Section: Discussionmentioning

confidence: 99%

Distribution of heat-shock protein 60 immunoreactivity in testes of infertile men

Werner¹,

Meinhardt

Seitz

et al. 1997

Cell and Tissue Research

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The immunohistochemical localization of heat-shock protein 60 (hsp60) was investigated in testicular biopsies obtained from 121 adult men with disturbed fertility. In normal unaffected tubules, hsp60 immunoreactivity was localized to spermatogonia, primary spermatocytes and Sertoli cells. In spermatogonia, cytosolic and mitochondrial labelling could be differentiated. In general, the number of stained spermatogonia decreased with the loss of spermatogenic function. A significant (P<0.01) reduction of stained spermatogonia was observed in testes with maturation arrest of spermatogenesis at the level of primary spermatocytes (30.2+/-21.6%) compared with testes exhibiting normal spermatogenesis. In addition, the decrease in the score correlated significantly with the diminution of cytosolic hsp60 immmunolabelling (coefficient r=0.25, P=0.03). There was a significant difference (P<0.01) in the percentage of cytosolic-stained spermatogonia in testes with a score equal to or greater than 5 (14.7+/-9.8%) and a score less than 5 (8. 9+/-6.9%). These observations suggest that a low level of hsp60 expression in spermatogonia may lead to a different pattern of protection, which in turn could be involved in low spermatogenic efficiency.

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“…In nonhuman primates, A dark and A pale spermatogonia are present in equal numbers and comprise 4% of germ cells in the testis (Marshall & Plant, 1996). Like nonhuman primates, A dark and A pale spermatogonia are present in equal numbers in the human testis (Clermont, 1966; Schulze, 1978; Paniagua et al, 1987) and constitute 22% of germ cells in the testis (Paniagua et al, 1987). Thus, the larger pool of stem/progenitor cells in the testes of higher primates compensates, in part, for the reduced number of transit amplifying divisions (Figs.…”

Section: The Pool Of Stem/progenitor Spermatogonia In Higher Primamentioning

confidence: 99%

Spermatogonial stem cells and spermatogenesis in mice, monkeys and men

2018

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Continuous spermatogenesis in post-pubertal mammals is dependent on spermatogonial stem cells (SSCs), which balance self-renewing divisions that maintain stem cell pool with differentiating divisions that sustain continuous sperm production. Rodent stem and progenitor spermatogonia are described by their clonal arrangement in the seminiferous epithelium (e.g., Asingle, Apaired or Aaligned spermatogonia), molecular markers (e.g., ID4, GFRA1, PLZF, SALL4 and others) and most importantly by their biological potential to produce and maintain spermatogenesis when transplanted into recipient testes. In contrast, stem cells in the testes of higher primates (nonhuman and human) are defined by description of their nuclear morphology and staining with hematoxylin as Adark and Apale spermatogonia. There is limited information about how dark and pale descriptions of nuclear morphology in higher primates correspond with clone size, molecular markers or transplant potential. Do the apparent differences in stem cells and spermatogenic lineage development between rodents and primates represent true biological differences or simply differences in the volume of research and the vocabulary that has developed over the past half century? This review will provide an overview of stem, progenitor and differentiating spermatogonia that support spermatogenesis; identifying parallels between rodents and primates where they exist as well as features unique to higher primates.

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Quantification of cell types throughout the cycle of the human seminiferous epithelium and their DNA content

Cited by 32 publications

References 10 publications

Prespermatogenesis and spermatogoniogenesis in the bovine testis

Prespermatogenesis and spermatogoniogenesis in the bovine testis

Distribution of heat-shock protein 60 immunoreactivity in testes of infertile men

Spermatogonial stem cells and spermatogenesis in mice, monkeys and men

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