Quantification of carbon flow from stable isotope fractionation in rice field soils with different organic matter content

Penning, Holger; Conrad, Ralf

doi:10.1016/j.orggeochem.2007.08.004

Cited by 61 publications

(63 citation statements)

References 43 publications

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“…Fractionation of acetate carbon is to be expected in systems in which acetate production is transiently high, such as in rice fields. In the methanogenic sediment of oligotrophic Lake Stechlin, d ac and d ac-methyl were similar in the presence or absence of CH 3 F (Conrad et al 2007). Such data indicate that there is no fractionation (a acÀmethyl=CH 4~1 ) during acetoclastic methanogenesis.…”

Section: Discussionmentioning

confidence: 72%

“…However, fractionation factors can vary considerably from one environment to the other, for example, because of different microbial communities (Chidthaisong et al 2002;Penning et al 2006a) or different energetic conditions for methanogenesis (Penning et al 2005;Valentine et al 2005). Thus, values of a CO 2 =CH 4 can vary between 1.03 and 1.09 (equivalent to e CO 2 =CH 4 of 290% to 230%).…”

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confidence: 99%

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Characterization of stable isotope fractionation during methane production in the sediment of a eutrophic lake, Lake Dagow, Germany

Conrad

Claus

Casper

2009

Limnology & Oceanography

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We measured d 13 C of CO 2 , CH 4 , and acetate-methyl in profundal sediment of eutrophic Lake Dagow by incubation experiments in the presence and absence of methanogenic inhibitors chloroform, bromoethane sulfonate (BES), and methyl fluoride, which have different specificities. Methyl fluoride predominantly inhibits acetoclastic methanogenesis and affects hydrogenotrophic methanogenesis relatively little. Optimization of methyl fluoride concentrations resulted in complete inhibition of acetoclastic methanogenesis. Methane was then exclusively produced by hydrogenotrophic methanogenesis and thus allowed determination of the fractionation factors specific for this methanogenic pathway. Acetate, which was then no longer consumed, accumulated and allowed determination of the isotopic signatures of the fermentatively produced acetate. BES and chloroform also inhibited CH 4 production and resulted in accumulation of acetate. The fractionation factor for hydrogenotrophic methanogenesis exhibited variability, e.g., it changed with sediment depth. The d 13 C of the methyl group of the accumulated acetate was similar to the d 13 C of sedimentary organic carbon, while that of the carboxyl group was by about 12% higher. However, the d 13 C of the acetate was by about 5% lower in samples with uninhibited compared with inhibited acetoclastic methanogenesis, indicating unusual isotopic fractionation. The isotope data were used for calculation of the relative contribution of hydrogenotrophic vs. acetoclastic methanogenesis to total CH 4 production. Contribution of hydrogenotrophic methanogenesis increased with sediment depth from about 35% to 60%, indicating that organic matter was only partially oxidized in deeper sediment layers.Eutrophic lakes usually develop an anoxic hypolimnion that lasts over much of the summer season. During the rest of the year, when the hypolimnion is oxic, only the top few centimeters of the sediment become oxidized while the rest remains reduced. In these sediments, production of CH 4 is the terminal step in the degradation of organic matter, and carbon cycling in the lakes is greatly affected by the release of CH 4 from the sediment into the water column (Rudd and Taylor 1980). Organic matter is fermented to acetate, H 2 , and CO 2 , which are subsequently converted to CH 4 . The relative contribution of acetate and H 2 plus CO 2 to CH 4 production can vary, and a mechanistic explanation for this behavior is not always available (Conrad 1999). The relative contribution of either acetoclastic or hydrogenotrophic methanogenesis also affects the d 13 C of the produced CH 4 (Whiticar 1999;Hornibrook et al. 2000). During hydrogenotrophic methanogenesis (4H 2 + CO 2 R CH 4 + 2H 2 O) the isotopically lighter carbon is strongly preferred, whereas the isotope effect is less expressed in acetoclastic methanogenesis (CH 3 COOH R CH 4 + CO 2 ), in which CH 4 is almost exclusively produced from the methyl group of the acetate (Weimer and Zeikus 1978;DeGraaf et al. 1996). Hence, the wide range of differ...

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Section: Discussionmentioning

confidence: 72%

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confidence: 99%

Characterization of stable isotope fractionation during methane production in the sediment of a eutrophic lake, Lake Dagow, Germany

Conrad

Claus

Casper

2009

Limnology & Oceanography

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“…Stable isotopic analyses of CH 4 and CO 2 were performed using a gas chromatograph combustion isotope ratio mass spectrometer (GC-C-IRMS) (Finnigan, Bremen, Germany) (Penning and Conrad, 2007). The determination of the stable isotopic signatures of dried plant (RS) and soil samples (SOM) was carried out at the Institute of Soil Science and Forest Nutrition (IBW) at the University of Göttingen, Germany.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Straw application in paddy soil enhances methane production also from other carbon sources

2014

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Abstract. Flooded rice fields are an important source of the greenhouse gas methane. Methane is produced from rice straw (RS), soil organic matter (SOM), and rice root organic carbon (ROC). Addition of RS is widely used for ameliorating soil fertility. However, this practice provides additional substrate for CH 4 production and results in increased CH 4 emission. Here, we found that decomposing RS is not only a substrate of CH 4 production, but in addition stimulates CH 4 production from SOM and ROC. Apart from accelerating the creation of reduced conditions in the soil environment, RS decomposition resulted in enhancement of SOM-derived CH 4 production. In particular, hydrogenotrophic methanogenesis from SOM-derived CO 2 was stimulated, presumably by H 2 released from RS decomposition. On the other hand, the enhancement of ROC-derived CH 4 production after RS application was probably caused by the significant increase of the abundance of methanogenic Archaea in the RS treatment compared with the untreated control. Our results show that traditional management of rice residues exerts a positive feedback on CH 4 production from rice fields, thus exacerbating its effect on the global CH 4 budget.

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“…Our team studied the yield of methane from organic matter passing through the digestive tract of horses and we found out that such a fear was little reasonable in most cases (Kolář et al, 2009b). The main sources of methane in the atmosphere are wetlands, landfills, peat bogs and paddy fields (Umemura et al, 2006;Penning and Conrad, 2007). In general, organic matter degradability is studied by specialists in animal nutrition, by forage specialists, compost producers and specialists in community hygiene and biogas production.…”

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Anaerobic degradability of organic matter of cattle faeces and a possibility of its utilization

Kolář¹,

Frelich²,

Brouček³

et al. 2010

CZECH J ANIM SCI

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AbstrAct:The content of organic matter labile fractions is negligible in cattle faeces but the content of the anaerobically degradable fraction on the boundary of organic matter lability and stability is analytically utilizable and different. It depends not only on feed quality and quantity but also on all factors of enteric fermentation and processes determining the digestion of ruminants. In the present paper we attempted to describe the digestive tract of ruminants as an anaerobic bioreactor and to calculate its mass balance so that the measurement of a difference in the anaerobic degradability of feeds and faeces at steady state could be theoretically demonstrated as a multipurpose research method not bothering the animals. The first results in eight variants proved that feed quality and other factors influencing processes in the digestive tract of ruminants could be monitored in this way. Obviously, this method does not replace the present methods used for feed digestibility determination.

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Quantification of carbon flow from stable isotope fractionation in rice field soils with different organic matter content

Cited by 61 publications

References 43 publications

Characterization of stable isotope fractionation during methane production in the sediment of a eutrophic lake, Lake Dagow, Germany

Characterization of stable isotope fractionation during methane production in the sediment of a eutrophic lake, Lake Dagow, Germany

Straw application in paddy soil enhances methane production also from other carbon sources

Anaerobic degradability of organic matter of cattle faeces and a possibility of its utilization

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