Quantification of Ca2+-ATPases in porcine duodenum. Effects of 1,25(OH)2D3 deficiency

Timmermans, Janneke; Kaune, R.; Bindels, René J. M.; Os, C.H. van

doi:10.1016/0005-2736(91)90228-z

Cited by 15 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For comparison, previously reported data (41) The reasons for the discrepancies between the findings from the above biochemical studies (42,43) and our studies are unknown at present. Although the anti-Ca2+ pump antibody (5F10) used in the previous immunological study (11) was produced against the human red cell membrane Ca2W pump, this antibody cross-reacts with the PMCA proteins of rat intestine (44), rat and human kidney (45,46), and human placenta (47); and, in our previous study (11), the 5F10 antibody immunohistochemically cross-reacted almost exclusively with a protein resident to the chicken intestinal basolateral membrane.…”

Section: Discussionmentioning

confidence: 36%

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

Cai

Chandler

Wasserman

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

155

View full text Add to dashboard Cite

The effect of vitamin D and other variables on the synthesis of the chicken intestinal plasma membrane calcium pump (PMCA) mRNA was assesed. The DNA probe for Northern analysis was obtained by reverse transcription and PCR with intestinal poly(A)+ RNA, using two 20-mer oligonucleotide primers homologous to the 3' coding region of the human teratoma PMCA. An EcoRI restriction fragent of the PCR product was cloned into the pBluescript I KS(-) phagemid vector, and the chimeric plasmid was used to transform Escherichia coli. The amino acid sequence deduced from the nucleotide DNA sequence of the PCR product and the cloned DNA were 96% homologous with the teratoma sequence. Northern blots of intestinal poly(A)+ RNA with 32P-labeled DNA showed the presence of three major species of chicken PMCA mRNAs at about 6.6, 5.4, and 4.5 kb. Northern analysis with the chicken PMCA DNA indicated that repletion of vitamin D-deficient chickens with vitamin D increased PMCA mRNAs in the duodenum, jejunum, ileum, and colon. After injection of 1,25-dihydroxyvitamin D3 intravenously into vitamin D-deflcient chickens, duodenal PMCA mRNA tended to increase by 2 hr, reached a maximum at about 16 hr, and returned to baseline levels at 48 hr. Adaptation of chickens to either a calcium-or phosphorus-deficient diet resulted in a 2-to 3-fold increase in duodenal PMCA mRNA. These results indicate that vitamin D and specific variables that affect calcium absorption through the vitamin D-endocrine system increase intestinal PMCA gene expression.A major factor controlling the efficiency of calcium absorption is the vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] (1, 2). 1,25(OH)2D3 elicits both genomic (3) and nongenomic (4-10) effects on the intestinal cell. Among the former effects are the induction of the synthesis of intestinal calbindin-D9k in mammalian species and intestinal calbindin-D28k in avian species (3). In addition, we recently demonstrated another apparent genomic response, a vitamin D-dependent increase in the amount ofcalcium pump units in the basolateral membrane of the avian enterocyte (11). This finding is consistent with previous observations (12)(13)(14)(15)(16) showing that repletion of vitamin D-deficient animals with vitamin D or 1,25(OH)2D3 results in an enhancement of the ATP-dependent uptake of Ca2+ by isolated basolateral membrane vesicles. Further, animals fed diets deficient in calcium or phosphorus adapt to these deficiencies by an increase in the synthesis of 1,25(0H)2D3 (17)(18)(19)(20)(21), resulting in an increase in the efficiency of calcium and phosphorus absorption. These adaptations are associated with an increased synthesis of calbindin-D and its mRNA (17)(18)(19)(20)(22)(23)(24)(25)(26)(27)(28)

show abstract

Section: Discussionmentioning

confidence: 36%

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

Cai

Chandler

Wasserman

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

155

View full text Add to dashboard Cite

show abstract

“…A direct effect on Ca2+ pump activities appears rather unlikely, for at least two reasons. Firstly, basal [Ca2+]i was identical in enterocytes from con-and def-animals indicating intracellular Ca2+ homeostasis including intact Ca2P pumps in both preparations, and secondly, it has been shown previously that Ca2+ pump capacities in basolateral membranes of porcine duodenum are not influenced by calcitriol deficiency (Timmermans et al 1991). An increased Ca2+ pump activity due to elevated [Ca2+]i in the deficient animals should be excluded since active transcellular Ca2+ transport was completely absent in duodenal tissues (Kaune et al 1992).…”

Section: Discussionmentioning

confidence: 65%

“…Purification was monitored by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (Laemmli, 1970) and fluorescence spectrometry (excitation, 276 nm; emission, 306 nm) (Bryant & Andrews, 1983). The competitive ELISA procedure has been described elsewhere (Timmermans, Kaune, Bindels & Van Os, 1991). Calbindin-Dgk concentrations are expressed as microgram calbindin-D9k per milligram of soluble protein, with 78 4 + 3 1 mg soluble protein (mean + S.D.…”

Section: Determination Of Mucosal Calbindin-dgk Concentrationsmentioning

confidence: 99%

Role of calbindin‐D9k in buffering cytosolic free Ca2+ ions in pig duodenal enterocytes.

Schröder

Schlumbohm

Kaune

et al. 1996

The Journal of Physiology

View full text Add to dashboard Cite

3. After addition of 5 mm theophylline, the [Ca2+]i in enterocytes from con-piglets doubled during the 10 min incubation. This effect, however, was three times higher in enterocytes from def-piglets compared with those from con-piglets. Similar results were obtained after 4 min incubation of enterocytes from con-and def-piglets in the presence of 1 /UM ionomycin. In preparations from def-D3-piglets, ionomycin-induced increases in [Ca2+]iwere significantly lower compared with enterocytes from def-piglets and were not different from the control values. 4. From the results, substantial support is given for the hypothesis that one of the major functions of mucosal calbindin-DAk is the effective buffering of Ca2+ ions.

show abstract

“…Basolateral efflux of Ca 2+ occurs against a considerable electrochemical gradient, which is mainly mediated via Ca 2+ -ATPase activity ("plasma membrane Ca 2+ -ATPase", PMCA1b; Sepulveda and Mata, 2004), with only a minor contribution of the Na + /Ca 2+ exchange (NCX) mechanism at least in pigs (Timmermans et al, 1991;Kaune, 1996;Hoenderop et al, 2000). Controversy still exists about the influence of calcitriol on basolateral Ca 2+ efflux.…”

Section: Calcium Absorptionmentioning

confidence: 98%

Transport of nutrients and electrolytes across the intestinal wall in pigs

Breves¹,

Kock²,

Schröder³

2007

Livestock Science

View full text Add to dashboard Cite

Quantification of Ca2+-ATPases in porcine duodenum. Effects of 1,25(OH)2D3 deficiency

Cited by 15 publications

References 28 publications

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

Role of calbindin‐D9k in buffering cytosolic free Ca2+ ions in pig duodenal enterocytes.

Transport of nutrients and electrolytes across the intestinal wall in pigs

Contact Info

Product

Resources

About