Quantification of Arbutin in Plant Extracts by Stable Isotope Dilution Gas Chromatography–Mass Spectrometry

Lee, K B; Choi, Jong-Chul; Ahn, Soon Kil; Na, J K; Shrestha, Kshitij; Nguon, Samnang; Park, S U; Choi, Sungjun; Kim, Jae Kwang

doi:10.1007/s10337-017-3461-5

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…Next, the developed LC-MS method based on [d 4 ]-arbutin as an IS was used to detect arbutin in Brigitta (0.43 ± 0.01 lg/mg of dry weight, DW) and Duke (0.40 ± 0.01 lg/mg of DW) blueberry leaves, as well as strawberry leaves (0.16 ± 0.02 lg/mg of fresh weight, FW) and pear peels (6.86 ± 1.39 lg/mg of DW). The results from these measurements were in agreement with previous studies, which report the concentrations of arbutin in blueberry leaves and pear peels to be 0.35 ± 0.01 lg/ mg of DW and in the range of 0.92-27.70 lg/mg of DW, respectively [17,35,36]. To the best of our knowledge, the concentration of arbutin in strawberry leaves (Fragaria spp.)…”

Section: Methods Validation Using Lc-mssupporting

confidence: 92%

“…Lamien-Meda et al [14] reported on the use of salicin as an IS in the GC-MS analysis of arbutin. In addition, another study presented a stable isotope dilution GC-MS analysis method using [d 4 ]-arbutin as an IS to overcome any problems connected to the stability of arbutin TMS derivatives [17]. The results from this method were then compared with the ones obtained when salicin was used as an IS.…”

Section: Matrix Effectmentioning

confidence: 99%

“…by trimethylsilylation, which reduces the polarity of the functional groups of arbutin and makes it volatile [16]. In addition, the compounds resulting from the derivatisation step are unstable over time and include multiple TMS derivatives such as 5TMS and 4TMS arbutin derivatives [17]. To overcome these problems, previous studies employed the stable isotope dilution method with [d 4 ]-arbutin as an internal standard (IS), which successfully eliminates the issues of multiple TMS derivatives with low stability [17].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, we analysed the matrix effect using two kinds of blueberry leaves, strawberry leaves, and pear peels. Finally, we compared this novel isotope dilution LC-MS method to the previously reported isotope dilution GC-MS method which employs the same SIL-IS [17].…”

Section: Introductionmentioning

confidence: 99%

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Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Kim

Park

et al. 2018

Appl Biol Chem

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Arbutin is a very safe whitening agent for human skin. Since it is more expensive than other agents and has a challenging synthesis, novel methods to obtain this valuable agent are needed. In this study, we developed a precise and accurate method to detect and quantify arbutin using stable isotope dilution liquid chromatography-mass spectrometry (LC-MS). One challenge that needed to be overcome was the matrix effect occurring during the LC-MS analysis due to the analyte ionisation enhancement or suppression in the electrospray ionisation source by coeluting compounds. Notably, arbutin had different matrix effects in the various sample matrices. A solution to this problem was the use of [d 4 ]-arbutin as a stable isotopelabelled internal standard (SIL-IS), as it compensated the matrix effect of arbutin because it was affected by almost the same matrix effect. The validation of the developed method showed excellent linearity (r 2 = 1.000), precision (relative standard deviation B 2.5%), accuracy (recovery, 97.42-98.52%), limit of detection (0.03 lg/mL), and limit of quantification (0.1 lg/mL). Finally, the method of arbutin detection was applied to blueberry leaves to compare the precision and accuracy results obtained by performing stable isotope dilution using LC-MS and gas chromatography-mass spectrometry. The method was applied to strawberry leaves and pear peels, indicating that the SIL-IS method can be expected to find application in the arbutin analysis in other plants.

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Section: Methods Validation Using Lc-mssupporting

confidence: 92%

Section: Matrix Effectmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Kim

Park

et al. 2018

Appl Biol Chem

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“…The helium gas flow rate, sample volume, and temperature program setting were as described by El Sherif et al (2020) . The computation of composition was according to Lee et al (2018) with slight modification.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the effects of Chlorella vulgaris, Nannochloropsis salina, and Enterobacter cloacae on growth, yield and active compound compositions of Moringa oleifera under salinity stress

dayel

Sherif

2021

Saudi Journal of Biological Sciences

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Application of Chlorella vulgaris , Nannochloropsis salina and Enterobacter cloacae has been reported to improve the growth of multiple plant species. Moringa oleifera is a medicinal plant found in Saudi Arabia. Its leaves, flowers and fruit have been used as food. Moringa oleifera is rich in rutin and gallic acid and many other bioactive compounds, which collectively contribute to its demonstrated range of pharmacological activities. In Saudi Arabia, the semi-arid and arid weather presents a significant challenge to agriculture. High salinity in cultivated land is a particular threat. We applied Chlorella vulgaris , Nannochloropsis salina , and Enterobacter cloacae at multiple salinities to Moringa oleifera to investigate their effects on the growth, yield, and photosynthetic pigment content. We also examined possible changes in the phytochemical composition. The application of Chlorella vulgaris , Nannochloropsis salina and Enterobacter cloacae enhanced plant growth and yield, while inhibition was observed at high (6000 ppm) salinity. The presence of Chlorella vulgaris and Nannochloropsis salina altered plant growth and yield and rutin and gallic acid content of Moringa oleifera plants grown in saline conditions. Microalgae species were recommended for use as a bio-fertiliser alternative to mainstream synthetic fertilisers.

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A comprehensive review of the therapeutic potential of α‐arbutin

Saeedi

Khezri

Zakaryaei

et al. 2021

Phytotherapy Research

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Cosmetic dermatology preparations such as bleaching agents are ingredients with skin‐related biological activities for increasing and improving skin beauty. The possibility of controlling skin hyperpigmentation disorders is one of the most important research goals in cosmetic preparations. Recently, cosmetics containing herbal and botanical ingredients have attracted many interests for consumers of cosmetic products because these preparations are found safer than other preparations with synthetic components. However, high‐quality trial studies in larger samples are needed to confirm safety and clinical efficacy of phytotherapeutic agents with high therapeutic index. Arbutin (p‐hydroxyphenyl‐β‐d‐glucopyranoside) is a bioactive hydrophilic polyphenol with two isomers including alpha‐arbutin (4‐hydroxyphenyl‐α‐glucopyranoside) and β‐arbutin (4‐hydroxyphenyl‐β‐glucopyranoside). It is used as a medicinal plant in phytopharmacy. Studies have shown that alpha‐arbutin is 10 times more effective than natural arbutin. A comparison of IC50 values showed that α‐arbutin (with concentration 2.0 mM) has a more potent inhibitory activity on human tyrosinase against natural arbutin (with higher concentration than 30 mM). A review of recent studies showed that arbutin could be beneficial in treatment of various diseases such as hyperpigmentation disorders, types of cancers, central nervous system disorders, osteoporosis, diabetes, etc. This study was designed to describe the therapeutic efficiencies of arbutin.

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Quantification of Arbutin in Plant Extracts by Stable Isotope Dilution Gas Chromatography–Mass Spectrometry

Cited by 7 publications

References 16 publications

Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Evaluation of the effects of Chlorella vulgaris, Nannochloropsis salina, and Enterobacter cloacae on growth, yield and active compound compositions of Moringa oleifera under salinity stress

A comprehensive review of the therapeutic potential of α‐arbutin

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