Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane

Vrkoslav, Vladimír; Smeringaiova, Ingrida; Smorodinova, Natalia; Svobodová, Alžběta; Strnad, Štěpán; Jackson, Catherine; Burkert, Jan; Jirsová, Kateřina

doi:10.3390/bioengineering10060740

Cited by 3 publications

(3 citation statements)

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“…Additional factors such as increased wound hydration, barrier protection, and decreased inflammation after application of hAM may also play a role in the reported attenuation of pain perception [6]. Additionally, N-acylethanolamides (NAEs) such as PEA, anandamide (AEA) and oleoylethanolamide (OEA) may play a role in the analgesic effects of hAM [11]. These NAEs are bioactive lipid mediators that play a role in immune function, pain and inflammation [11].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, N-acylethanolamides (NAEs) such as PEA, anandamide (AEA) and oleoylethanolamide (OEA) may play a role in the analgesic effects of hAM [11]. These NAEs are bioactive lipid mediators that play a role in immune function, pain and inflammation [11]. AEA alleviates pain by interacting with cannabinoid-1 receptors in the periphery as a powerful antinociceptive [12].…”

Section: Discussionmentioning

confidence: 99%

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The Use of Human Amniotic Membrane Tissue Grafting in Acute Traumatic Finger Injury: A Case Report

Perez,

Basra,

Patel

et al. 2024

Cureus

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Human amniotic membrane (hAM) is a collagen-based extracellular matrix that facilitates regenerative wound care. hAM offers several advantageous properties that promote epithelial cell growth, granulation, and angiogenesis. This case report demonstrates how Vivex Cygnus Matrix (Vivex Biologics, Miami, FL, USA) amniotic membrane was used over four weeks to graft a traumatic index finger injury that occurred while fishing. Cygnus Matrix allograft was first placed 72 hours after the accident. Following graft placement, the patient noted an immediate relief in pain and was able to return to all normal daily work activities within 48 hours of graft placement. Granulation tissue appeared a few days later. A total of four grafts were placed over the course of four weeks starting on September 4th, 2023. Typically, acute traumatic wounds are managed with a regimen of irrigation, wound dressing, and debridement. In this unique case, a distal fingertip amputation was treated with Cygnus Matrix allograft. A single hAM was applied weekly over the course of four weeks. Complete reepithelization of the injury was achieved with minimal scar formation. This paper demonstrates the use of hAM in healing acute traumatic wounds as an effective alternative to other more traditional treatments such as skin grafting, surgical reimplantation, and composite grafting. Utilization of hAM in acute traumatic wounds has few research reports that assure that the applications have minimal drawbacks while at the same time promoting wound management and patient comfort.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Use of Human Amniotic Membrane Tissue Grafting in Acute Traumatic Finger Injury: A Case Report

Perez,

Basra,

Patel

et al. 2024

Cureus

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“…The analgesic effect of cryopreserved AM was mainly explained by the reduction of inflammation and thus secondary to pain relief [ 31 , 54 ]. Recently endogenous bioactive lipids with significant analgesic and nociceptive effects were detected in both placental membranes prepared for grafting [ 25 , 55 ]. It is clear that also anti-inflammatory components present in AM indirectly contribute to the analgesic effect of AM.…”

Section: Discussionmentioning

confidence: 99%

Outcome of Application of Cryopreserved Amniotic Membrane Grafts in the Treatment of Chronic Nonhealing Wounds of Different Origins in Polymorbid Patients: A Prospective Multicenter Study

Svobodova,

Horvath,

Balogh

et al. 2023

Bioengineering

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To compare the therapeutic efficacy of cryopreserved amniotic membrane (AM) grafts and standard of care (SOC) in treating nonhealing wounds (NHW) through a prospective multicenter clinical trial, 42 patients (76% polymorbid) with 54 nonhealing wounds of various etiologies (mainly venous) and an average baseline size of 20 cm2 were included. All patients were treated for at least 6 weeks in the center before they were involved in the study. In the SOC group, 29 patients (36 wounds) were treated. If the wound healed less than 20% of the baseline size after 6 weeks, the patient was transferred to the AM group (35 patients, 43 wounds). Weekly visits included an assessment of the patient’s condition, photo documentation, wound debridement, and dressing. Quality of life and the pain degree were subjectively reported by patients. After SOC, 7 wounds were healed completely, 1 defect partially, and 28 defects remained unhealed. AM application led to the complete closure of 24 wounds, partial healing occurred in 10, and 9 remained unhealed. The degree of pain and the quality of life improved significantly in all patients after AM application. This study demonstrates the effectiveness of cryopreserved AM grafts in the healing of NHW of polymorbid patients and associated pain reduction.

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Potential novel role of the human amniotic membrane as a sustainable hemostat

Mousa,

Soliman,

Al‐Mofty

2024

Intl J Gynecology & Obste

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ObjectivesAcute hemorrhage can cause significant morbidity and mortality arising from trauma, bleeding disorders, surgical procedures, or obstetric complications. Surgical hemostasis methods may fail to stop acute bleeding due to the complex bleeding dynamics of each bleeding type. Therefore, developing safe and effective topical hemostatic agents remains crucial. The human amniotic membrane (hAM) has established clinical evidence of effectiveness in promoting wound healing and tissue regeneration. Despite its unique biological and immunologic properties and its structural composition of established hemostatic elements, the hemostatic role of hAM has not been yet explored. The present study aimed to investigate this potential role and to describe the development protocol and characterization of hAM‐derived topical hemostat.MethodsSurface electron microscope (SEM) imaging and Fourier transform infrared (FTIR) spectroscopy were used for characterization, and mouse models with induced peritoneal and tail wound bleeding were employed to evaluate the hemostatic effectiveness using physiological studies, in comparison to a chitosan‐based combat‐scale hemostat.ResultsThe hAM hemostat showed a distinctive composition by SEM and FTIR. Applying equal masses of the hAM hemostat, the commercial hemostat, or a combination reduced peritoneal wound bleeding time to averages of 108.4, 86.2, and 76.8 s, respectively, compared to the control group (300 s). Tail wound bleeding times were similarly reduced with no significant difference between the hAM and the commercial hemostat (P values = 0.29, 0.34 in peritoneal and tail wounds, respectively). Neither hemostat affected coagulation time.ConclusionThis study describes a simple cost‐effective preparation protocol for a hAM‐based hemostatic agent. The long‐recognized safety, sustainability, and immunotolerance advantages of hAM can establish superiority over commercial hemostats with reported safety concerns. Robust research validation in larger‐scale bleeding models is required for wider applications and severe bleeding types.

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Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane

Cited by 3 publications

References 63 publications

The Use of Human Amniotic Membrane Tissue Grafting in Acute Traumatic Finger Injury: A Case Report

The Use of Human Amniotic Membrane Tissue Grafting in Acute Traumatic Finger Injury: A Case Report

Outcome of Application of Cryopreserved Amniotic Membrane Grafts in the Treatment of Chronic Nonhealing Wounds of Different Origins in Polymorbid Patients: A Prospective Multicenter Study

Potential novel role of the human amniotic membrane as a sustainable hemostat

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