Quantification of acetylcholinesterase-positive structures in human thymus during development and aging

Cavallotti, Daniela; Artico, Marco; Iannetti, Gian Domenico; Cavallotti, Carlo

doi:10.1016/s0197-0186(99)00090-x

Cited by 9 publications

(12 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In both humans and rats, the aging thymus undergoes programmed deterioration correlated with increased thymic AChE expression, 25 in accordance with our results with the common anti-AChE antibodies. Previous research showed an age-related increase in serum AChE.…”

Section: Ache-r Changes During Thymus Agingsupporting

confidence: 92%

“…20 Moreover, both AChE-R and its naturally cleavable C-terminal domain showed powerful proliferative effects in blood cells 21,22 and tight interactions through its unique C-terminal domain with the scaffold protein RACK1 and through it with protein kinase C (PKC)␤II 23 or PKC⑀. 24 Because AChE is present in thymus, 25 which is central in MG appearance, we hypothesized that AChE variants, and especially AChE-R, could causally participate in the myasthenic syndrome through an action on developing T cells. To test this hypothesis, we analyzed the expression and function of AChE in the human normal and pathologic thymus and explored the influence of overexpression of AChE on murine thymic development.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The thymic theme of acetylcholinesterase splice variants in myasthenia gravis

Gilboa-Geffen

Lacoste

Soreq

et al. 2007

Blood

View full text Add to dashboard Cite

Section: Ache-r Changes During Thymus Agingsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The thymic theme of acetylcholinesterase splice variants in myasthenia gravis

Gilboa-Geffen

Lacoste

Soreq

et al. 2007

Blood

View full text Add to dashboard Cite

“…There are no fibrillary components of thymus parenchyma that cause aspecific reactions and limit Bodian technique specificity in marking nerve fibers. 17,31,33 …”

Section: Discussionmentioning

confidence: 99%

“…In fact, an unquestionable neuroanatomical evidence of a parasympathetic or vagal nerve supply to the thymus has not yet been found. Whilst some authors have reported anatomical mapping on vagal hindbrain nuclei, 16 or the presence of acethylcholinesterase-positive structures, 17,18 others have not confirmed these findings 9,19 and sentence about a substantial absence of any vagal nerve supply to any immune organs. 8 Similar disagreements concern data interpretation following the use of fluorescent tracer and horseradish peroxidase retrograde labelling 16 or retrograde transneuronal virus tracing.…”

Section: Introductionmentioning

confidence: 97%

Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus

et al. 2010

View full text Add to dashboard Cite

The aim of this study was to examine rat thymus innervation using denervation techniques and to explore the related microanatomical localization of dopamine, D1, D2 receptors and dopamine membrane transporter (DAT).In the thymus subcapsular region, the parenchymal cholinergic fibers belong exclusively to phrenic nerve branching. No somatic phrenic nerve branching was detected in any other analysed thymus lobule regions.In rats subjected to sympathetic or parasympathetic ablation, it was observed that catecholaminergic and cholinergic nerve fibers respectively contributed to forming plexuses along vessel walls.In the subcapsular and septal region, no parenchymal nerve branching, belonging to sympathetic or parasympathetic nervous system was noted. Instead, in the deep cortical region, cortico-medullary junction (CM-j) and medulla, catecholaminergic and cholinergic nerve fibers were detected along the vessels and parenchyma.Dopamine and dopamine receptors were widely diffused in the lobular cortico-medullary junction region and in the medulla, where the final steps of thymocyte maturation and their trafficking take place.No variation in dopamine and DAT immune reaction was observed following total or partial parasympathectomy or phrenic nerve cutting. After chemical or surgical sympathectomy however, neither dopamine nor DAT immune reaction was noted again. Instead, D1 and D2 dopamine receptor expression was not affected by thymus denervation.In rats subjected to specific denervation, it was observed the direct intraparenchymal branching of the phrenic nerve and sympathetic and parasympathetic fibers into thymus parenchyma along vessels. These findings on the dopaminergic system highlight the importance of neurotransmitter receptor expression in the homeostasis of neuroimmune modulation.

show abstract

“…In addition to sympathetic innervation, the thymus contains nerve fibers containing acetyl-cholinesterase (Bulloch et al, 1991;Cavallotti et al, 2000) or GABAtransaminase (Cavallotti et al, 1999b), as well as peptidergic nerves containing calcitonin gene-related peptide (CGRP) and neuropeptide Y (Bulloch et al, 1991;Kranz et al 1997) (Fig. 3).…”

Section: Innervation Of the Mammalian Thymusmentioning

confidence: 99%

Age‐dependent changes in the nervous and endocrine control of the thymus

Hannestad¹,

Monjil²,

Díaz-Esnal³

et al. 2004

Microscopy Res & Technique

View full text Add to dashboard Cite

The immune system, especially the thymus, undergoes age-related modifications leading to structural and functional changes in the lymphoid organs and immunocompetent cells. Nevertheless, the consequences of thymic involution in the peripheral pool of T-cells are still a matter of controversy. The control of the thymic function is very complex and involves intrathymic signals, the autonomic nervous system, and the endocrine system. Both thymocytes and thymic stromal cells express receptors for a wide range of hormones, as well as for neurotransmitters and neuropeptides, thus affecting thymocytes maturation. This review summarizes the age-dependent variations in the extrathymic components of the thymic microenvironment, i.e., vegetative nerves and hormones, and the possible effects of those changes in the immune function.

show abstract

Quantification of acetylcholinesterase-positive structures in human thymus during development and aging

Cited by 9 publications

References 13 publications

The thymic theme of acetylcholinesterase splice variants in myasthenia gravis

The thymic theme of acetylcholinesterase splice variants in myasthenia gravis

Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus

Age‐dependent changes in the nervous and endocrine control of the thymus

Contact Info

Product

Resources

About