Quantification of acesulfame potassium in processed foods by quantitative 1H NMR

Ohtsuki, Takashi; Sato, Kyoko; Abe, Yutaka; Sugimoto, Naotoshi; Akiyama, Hiroshi

doi:10.1016/j.talanta.2014.08.002

Cited by 41 publications

(25 citation statements)

References 23 publications

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“…All integrated values in this study were expressed in terms of purity (%). The purity of the reagents was calculated using the following formula based on a previous study [8][9][10][11] :…”

Section: Chemical and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

Absolute Purity Determination of a Hygroscopic Substance, Indocyanine Green, Using Quantitative NMR (qNMR)

et al. 2021

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Quantitative NMR (qNMR) is applied to determine the absolute quantitative value of analytical standards for HPLC-based quantification. We have previously reported the optimal and reproducible sample preparation method for qNMR of hygroscopic reagents, such as saikosaponin a, which is used as an analytical standard in the assay of crude drug section of Japanese Pharmacopoeia (JP). In this study, we examined the absolute purity determination of a hygroscopic substance, indocyanine green (ICG), listed in the Japanese Pharmaceutical Codex 2002, using qNMR for standardization by focusing on the adaptation of ICG to JP. The purity of ICG, as an official non-Pharmacopoeial reference standard (non-PRS), had high variation (86.12 ± 2.70%) when preparing qNMR samples under non-controlled humidity (a conventional method). Additionally, residual ethanol (0.26 ± 0.11%) was observed in the non-PRS ICG. Next, the purity of non-PRS ICG was determined via qNMR when preparing samples under controlled humidity using a saturated sodium bromide solution. The purity was 84.19 ± 0.47% with a lower variation than that under non-controlled humidity. Moreover, ethanol signal almost disappeared. We estimated that residual ethanol in non-PRS ICG was replaced with water under controlled humidity. Subsequently, qNMR analysis was performed when preparing samples under controlled humidity in a constant temperature and humidity box. It showed excellent results with the lowest variation (82.26 ± 0.19%). As the use of a constant temperature and humidity box resulted in the lowest variability, it is recommended to use the control box if the reference ICG standard is needed for JP assays.

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“…All integrated values in this study were expressed in terms of purity (%). The purity of the reagents was calculated using the following formula based on a previous study [8][9][10][11] :…”

Section: Chemical and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

Absolute Purity Determination of a Hygroscopic Substance, Indocyanine Green, Using Quantitative NMR (qNMR)

et al. 2021

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“…It is also applied to evaluate food processing, for example, monitoring lactic acid production during milk fermentation [230], estimating nonthermal processing of orange juice [217]. In addition, food additives [246] and packaging material [245] can also be evaluated by 1 H qNMR. Food authenticity detection or adulteration analysis is an important application field of qNMR, which is well demonstrated by recent published literatures [235,239,248].…”

Section: Food Analysismentioning

confidence: 99%

Quantitative NMR Studies of Multiple Compound Mixtures

2017

Annual Reports on NMR Spectroscopy

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“…Quantitative 1 H NMR spectra were recorded at room temperature (21-25°C). Each spectrum comprised 64 000 data points with 16 scan times, an acquisition time of 4.1 s, a recycle delay of 60 s, and a pulse angle of 90°to facilitate complete relaxation and absolute quantification (Ohtsuki et al, 2014). The spectra were acquired with automatic sample loading and shimming, and were Fourier transformed with 0.2 Hz line broadening.…”

Section: Qnmr Analyses Of Phlorotanninsmentioning

confidence: 99%

Diversity of phlorotannin profiles among sargassasacean species affecting variation and abundance of epiphytes

Nakajima

Sugimoto

Ohki

et al. 2016

European Journal of Phycology

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In general, epiphytes have detrimental effects on the growth of their basiphytes due to competition for light and nutrients. Therefore, basiphyte species must expend energy suppressing epiphytes. Some studies suggest that phlorotannins, i.e. brown algal polyphenols, prevent colonization by epiphytes, whereas others question their allelopathic function because there is not necessarily a negative correlation between epiphyte abundance and the phlorotannin content of the basiphyte algae. Various phlorotannin components are found in brown algal species, thus we hypothesized that the antifouling activities of polyphenolic compounds may differ and that the analysis of phlorotannin profiles could be useful for estimating their ecological functions. We surveyed the epiphyte richness in the apical portions of 373 thalli from 15 sargassacean species, demonstrating that the variation and abundance of epiphyte species differed remarkably among the basiphyte species. However, there was a weak negative correlation between the density and total phlorotannin content of the basiphyte algae in only one of the 18 epiphyte species. The interspecific differences in the phlorotannin profile were characterized by quantitative 1 H nuclear magnetic resonance spectroscopy (qNMR), and four major groups were categorized based on cluster and principal component analyses of polyphenolic signals in the qNMR spectra. The epiphyte Neosiphonia harveyi was more abundant on Sargassum hemiphyllum, S. patens and S. piluliferum than on other basiphyte species, and these three species were similar according to the cluster analysis. These results suggest that some phlorotannin components may be more effective for antifouling; thus interspecific differences in the phlorotannin profile could affect the variation and abundance of epiphytes.

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Quantification of acesulfame potassium in processed foods by quantitative 1H NMR

Cited by 41 publications

References 23 publications

Absolute Purity Determination of a Hygroscopic Substance, Indocyanine Green, Using Quantitative NMR (qNMR)

Absolute Purity Determination of a Hygroscopic Substance, Indocyanine Green, Using Quantitative NMR (qNMR)

Quantitative NMR Studies of Multiple Compound Mixtures

Diversity of phlorotannin profiles among sargassasacean species affecting variation and abundance of epiphytes

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