Quantification of 15 bile acids in lake charr feces by ultra-high performance liquid chromatography–tandem mass spectrometry

Li, Ke; Buchinger, Tyler J.; Bussy, Ugo; Fissette, Skye D.; Johnson, Nicholas S.; Li, Weiming

doi:10.1016/j.jchromb.2015.07.028

Cited by 17 publications

(21 citation statements)

References 30 publications

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“…To determine the most prominent bile acid signals in the washings, a nontarget (broad‐spectrum) full‐scan analysis was conducted using UPLC‐HRMS following the established methods of Li et al . (). Nontargeted full‐scan analysis acquires signals from all compounds present within the sample, which is different from a targeted analysis, which only acquires specific compound signals.…”

Section: Methodsmentioning

confidence: 98%

American eels produce and release bile acid profiles that vary across life stage

Schmucker

Johnson

Bussy

et al. 2020

Journal of Fish Biology

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The American eel (Anguilla rostrata) is an imperilled fish hypothesized to use conspecific cues, in part, to coordinate long‐distance migration during their multistage life history. Here, holding water and tissue from multiple American eel life stages was collected and analysed for the presence, profile and concentration of bile acids. Distinct bile acid profiles were identified in glass, elver, yellow eel and silver eel holding waters using ultraperformance liquid chromatography high‐resolution mass spectrometry and principal component analysis. Taurochenodeoxycholic acid, taurodeoxycholic acid, cholic acid, deoxycholic acid, taurolithocholic acid and taurocholic acid were detected in whole tissue of American glass eels and elvers, and in liver, intestine and gallbladder samples of late‐stage yellow eels. Bile acids were not a major component of silver eel washings or tissue. This study is novel because little was previously known about bile acids produced and emitted into the environment by American eels. Future behavioural studies could evaluate whether any bile acids produced by American eels influence conspecific migratory behaviour.

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Section: Methodsmentioning

confidence: 98%

American eels produce and release bile acid profiles that vary across life stage

Schmucker

Johnson

Bussy

et al. 2020

Journal of Fish Biology

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“…We quantified 16 bile acids hypothesized to guide adult lake trout to spawning reefs (Zhang et al., ) to determine their presence as odorants during the spawning season (Li et al., ). We added an internal standard of 10 ng deuterated cholic acid and taurocholic acid (CA‐d 4 and TCA‐d 4 ) to a 10 ml subsample of the 1 L samples collected from aquaria over 16 weeks, as described above.…”

Section: Methodsmentioning

confidence: 99%

“…Each sample was freeze‐dried using a CentriVap Cold Trap with CentriVap Concentrator (Labconco, MO) and reconstituted in 100 μl of methanol and water (1:1, v:v). Reconstituted samples were subjected to UHPLC‐MS/MS (Waters Acquity ultra‐performance liquid chromatography system, Waters, Milford, MA; Micromass Quattro Premier XE tandem quadruple mass spectrometer, Waters, Manchester, UK) using described methods (Li et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…We freeze‐dried 20 L of aqueous fry feces to 1.1 kg, estimated to be roughly equivalent to that produced by 5,000 fry over two months (4 g feces/kg fish per day; Clark, Harman, & Forster, ). Using an established method (Li et al., ), we extracted bile acids from fry feces and an equal weight of food pellets (BioVita Starter #0, Bio‐Oregon http://www.bio‐oregon.com, and Fry crumble #1, Skretting http://www.skretting.us). Dry feces or food was resuspended in 3 L of ethanol, sonicated, refluxed, centrifuged, the supernatant removed, and the process repeated.…”

Section: Methodsmentioning

confidence: 99%

“…Lastly, the pooled supernatant was evaporated and reconstituted in 2 L ethanol. Triplicate 1 ml samples were dried and used to determine the concentrations of bile acids (reported by Li et al., ). We mixed the extracted bile acids into eight PEG emitters (per treatment) designed to release bile acids slowly over 15 days.…”

Section: Methodsmentioning

confidence: 99%

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Temporal constraints on the potential role of fry odors as cues of past reproductive success for spawning lake trout

Buchinger

Marsden

Binder

et al. 2017

Ecology and Evolution

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Deciding where to reproduce is a major challenge for most animals. Many select habitats based upon cues of successful reproduction by conspecifics, such as the presence of offspring from past reproductive events. For example, some fishes select spawning habitat following odors released by juveniles whose rearing habitat overlaps with spawning habitat. However, juveniles may emigrate before adults begin to search for spawning habitat; hence, the efficacy of juvenile cues could be constrained by degradation or dissipation rates. In lake trout (Salvelinus namaycush), odors deposited by the previous year's offspring have been hypothesized to guide adults to spawning reefs. However, in most extant populations, lake trout fry emigrate from spawning reefs during the spring and adults spawn during the fall. Therefore, we postulated that the role of fry odors in guiding habitat selection might be constrained by the time between fry emigration and adult spawning. Time course chemical, physiological, and behavioral assays indicated that the odors deposited by fry likely degrade or dissipate before adults select spawning habitats. Furthermore, fry feces did not attract wild lake trout to constructed spawning reefs in Lake Huron. Taken together, our results indicate fry odors are unlikely to act as cues for lake trout searching for spawning reefs in populations whose juveniles emigrate before the spawning season, and underscore the importance of environmental constraints on social cues.

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Plasma metabolomic profiles reveal sex- and maturation-dependent metabolic strategies in sea lamprey (Petromyzon marinus)

et al. 2022

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Introduction Adult sea lamprey (Petromyzon marinus) cease feeding and migrate to spawning streams where males build nests, undergo nal sexual maturation, and subsequently produce and release large quantities of bile acid pheromones that attract mature females. These animals are predicted to rearrange their metabolic pathways drastically to support their reproductive strategies, presenting advantageous opportunities to examine how sex and the maturation processes affect metabolism.Objectives The objective is to investigate the metabolic differences between sexes and maturation states in sea lamprey that support changes in physiological functions.Methods We compared plasma metabolomes of spawning and prespawning sea lamprey in both sexes using both non-targeted and targeted metabolomics approaches using UPLC/MS-MS with electrospray ionization in both positive and negative modes. The data were processed using Progenesis QI, Compound Discoverer and XCMS softwares for alignment, peak picking, and deconvolution of the peaks. Principle component analyses (PCA) and partial least squares discriminant analyses (PLS-DA) were performed using SIMCA and Metaboanalyst softwares to identify discriminating features, followed by fragmentation matching with extensive database search and pathway mapping. ResultsThe pheromonal bile acid biosynthesis was upregulated signi cantly in males compared to females. Spermiating males further upregulated bile acid biosynthesis by altering amino acid metabolisms, upregulating cofactors and nucleotide metabolisms, but downregulating carbohydrate and energy metabolisms.Conclusion Plasma metabolomes are sex-and maturation-dependent and re ect the special metabolic demands at each life stage and reproductive strategy.

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Quantification of 15 bile acids in lake charr feces by ultra-high performance liquid chromatography–tandem mass spectrometry

Cited by 17 publications

References 30 publications

American eels produce and release bile acid profiles that vary across life stage

American eels produce and release bile acid profiles that vary across life stage

Temporal constraints on the potential role of fry odors as cues of past reproductive success for spawning lake trout

Plasma metabolomic profiles reveal sex- and maturation-dependent metabolic strategies in sea lamprey (Petromyzon marinus)

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