Quantification anomalies in single pulse LA-ICP-MS analysis associated with laser fluence and beam size

Jerše, Ana; Mervič, Kristina; Elteren, Johannes T. van; Šelih, Vid Simon; Šala, Martin

doi:10.1039/d2an01172g

Cited by 9 publications

(8 citation statements)

References 32 publications

(66 reference statements)

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“…Selective ablation of the gelatin microdroplets and tissue sections was achieved by selecting an energy density below the ablation threshold of glass and above the ablation threshold of gelatin . Gelatin microdroplets and tissue sections were removed quantitatively using a fluence of 0.60 J cm –2 …”

Section: Methodsmentioning

confidence: 99%

Semiquantitative Analysis for High-Speed Mapping Applications of Biological Samples Using LA-ICP-TOFMS

et al. 2023

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Laser ablation (LA) in combination with inductively coupled plasma time-of-flight mass spectrometry (ICP-TOFMS) enables monitoring of elements from the entire mass range for every pixel, regardless of the isotopes of interest for a certain application. This provides nontargeted multi-element (bio-)imaging capabilities and the unique possibility to screen for elements that were initially not expected in the sample. Quantification of a large range of elements is limited as the preparation of highly multiplexed calibration standards for bioimaging applications by LA-ICP-(TOF)MS is challenging. In this study, we have developed a workflow for semiquantitative analysis by LA-ICP-TOFMS based on multi-element gelatin micro-droplet standards. The presented approach is intended for the mapping of biological samples due to the requirement of matrix-matched standards for accurate quantification in LA-ICPMS, a prerequisite that is given by the use of gelatin-based standards. A library of response factors was constructed based on 72 elements for the semiquantitative calculations. The presented method was evaluated in two stages: (i) on gelatin samples with known elemental concentrations and (ii) on real-world samples that included prime examples of bioimaging (mouse spleen and tumor tissue). The developed semiquantification approach was based on 10 elements as calibration standards and provided the determination of 136 nuclides of 63 elements, with errors below 25%, and for half of the nuclides, below 10%. A web application for quantification and semiquantification of LA-ICP(-TOF)MS data was developed, and a detailed description is presented to easily allow others to use the presented method.

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Section: Methodsmentioning

confidence: 99%

Semiquantitative Analysis for High-Speed Mapping Applications of Biological Samples Using LA-ICP-TOFMS

et al. 2023

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“…Ionisation of Cu and Zn in solution resulted in the formation of [M(NO 3 ) x (H 2 O) y (OH) z ] + complex ions in the MPT. These ions were then subject to MS 4 multi-stage fragmentation using CID to obtain the ions 63 Cu + and 66 Zn + which yielded LODs of 0.23 and 1.1 mg L −1 , respectively.…”

Section: Instrumentationmentioning

confidence: 99%

“…Additional uncertainties associated with laser fluence and beam size using various ablation cells and interfaces make quantification even more challenging. These additional factors are considered by Jerse et al 63 using approaches based on pulse intensity, LA spot volumes and noise characteristics for As, Gd, La, Ni, Te and Zn. A range of analyte concentrations (between 10 and 1000 μg g −1 ), and matrices (gelatine standards and NIST SRM 612) were studied.…”

Section: Solids Analysismentioning

confidence: 99%

Atomic spectrometry update: review of advances in atomic spectrometry and related techniques

Evans

Pisonero

Smith

et al. 2023

J. Anal. At. Spectrom.

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“…4, it could be found that the analytical precision of Cr and Cd is poorer than that of Pb. In addition to the relatively low signal-to-noise ratio of these two elements, some studies have also pointed out that it may be affected by the carbon loading in the ICP and ablated particle size, 41,42 resulting in uctuations and nonlinear shis in the signal intensity in the long-term analysis. 43 This eventually resulted in measured RSD values of 1.5%, 3%, and 2.5% for Pb, Cd, and Cr elements, respectively.…”

Section: Evaluation Of Homogeneity By La-icp-msmentioning

confidence: 99%

The production of polymer reference materials for microanalysis with high homogeneity by a 3D printing method

Gao

Ren

Zhou

et al. 2023

J. Anal. At. Spectrom.

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Quantification anomalies in single pulse LA-ICP-MS analysis associated with laser fluence and beam size

Abstract: Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has undergone major improvements in recent years which have led to reduction of the analysis time, higher spatial resolution, and better sensitivity....

Cited by 9 publications

References 32 publications

Semiquantitative Analysis for High-Speed Mapping Applications of Biological Samples Using LA-ICP-TOFMS

Semiquantitative Analysis for High-Speed Mapping Applications of Biological Samples Using LA-ICP-TOFMS

Atomic spectrometry update: review of advances in atomic spectrometry and related techniques

The production of polymer reference materials for microanalysis with high homogeneity by a 3D printing method

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