Quantification and Proteomic Characterization of β-Hydroxybutyrylation Modification in the Hearts of AMPKα2 Knockout Mice

Proteomics and β-hydroxybutyrylation Modification Characterization in the Hearts of Naturally Senescent Mice

“…Succinate-CoA ligase (ADP-forming) subunit beta (Sucla2) participated in the mitochondrial TCA cycle and was found to be significantly downregulated. Kbhb proteomics analysis in AMPKα2 knockout mice also revealed that most of the Kbhb-modified proteins are located in the mitochondria, which is consistent with this present study ( 10 ). Thus, we deduced that the mitochondrial proteins exhibit remarkable changes in Kbhb modification, and mitochondria are the primary locations of Kbhb modification in aged hearts.…”

“…Similar to that observed for Kbhb in the hearts of AMPK knockout mice ( 10 ), several pathways related to energy metabolism were enriched in Kbhb, including fatty acid β-oxidation, glyoxylate, and dicarboxylate metabolism, TCA cycle, and oxidative phosphorylation. Several critical enzymes have at least one Kbhb site.…”

“…To identify and quantify specific β-hydroxybutyrylated lysine sites in the proteins in aged heart tissue, we used antibody-based enrichment of β-hydroxybuturylated peptides followed by the DDA proteomics technique as described previously ( 10 ). Subsequently, we identified 1710 β-hydroxybutyrylated lysine sites in 641 proteins in the cardiac tissue from young and aged mice.…”

“…The grip strength of the mice’s front paws was measured using a grip strength meter (Jiangsu Science Biological Technology Co., Ltd, SANS, SA417). The measurements were recorded at 3 and 22 months, as described previously ( 10 ). Each mouse underwent five trials, and the final grip strength value per mouse was recorded as the average of the top three trials.…”

Multi-omics reveals aging-related pathway in natural aging mouse liver

“…The steps were described in our previous study [ 20 ]. The primary antibodies against BRAF (proteintech), MEK (Abcam), ERK (proteintech), GAPDH (proteintech), and the mouse IgG (H + L) secondary antibody (Pierce, Rockford, USA), rabbit IgG (H + L) secondary antibody (Pierce, Rockford, USA) were used.…”