Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry

Soares, Márcio Santos; Silva, Danielle Fernandes da; Forim, Moacir Rossi; Silva, Maria Fátima das Graças Fernandes da; Fernandes, João B.; Vieira, Paulo C.; Silva, Denise Brentan; Lopes, Norberto Peporine; Carvalho, Sérgio Alves de; Souza, Alessandra Alves de; Machado, Marcos Antônio

doi:10.1016/j.phytochem.2015.02.011

Cited by 62 publications

(43 citation statements)

References 28 publications

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“…As a result, it was found that hesperidin, which is found in great amounts in cells of the mesophyll of the affected leaves with CVC, shows a moderate activity suggesting that it can act as a good barrier for small-size colonies from X. fastidiosa. 28,29 Therefore, the search for novel semisynthetic modifications of some flavonoids has attracted our attention. One strategy would be to promote metal chelation of flavonoids and study the biological potential of these interactions.…”

Section: Resultsmentioning

confidence: 99%

An Environmentally Friendly Procedure to Obtain Flavonoids From Brazilian Citrus Waste

Bellete¹,

Ramin²,

Porto³

et al. 2018

J. Braz. Chem. Soc.

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Currently, most food waste is used as animal feed and this process does not take advantage of the available chemical composition. A Brazilian example is the citrus fruit processing wastes (CFPW), which have begun to draw attention due to their biological importance. In order to access the main compounds of this matrix, an efficient and environmentally friendly procedure was tested. From this extract, flavonoids as naringenin, hesperitin, chrysoeriol, sinensetin, 3,5,6,7,3',4'-hexamethoxyflavone, nobiletin, 5-methoxysalvigenin, 3,5,6,7,8,3',4'-heptamethoxyflavone, 3,5,6,7,4'-pentamethoxyflavone and isosakuranetin were identified by high performance liquid chromatography (HPLC-UV), liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-nuclear magnetic resonance (LC-NMR). This study describes the potential use of citrus waste as a source of biologically active compounds. The extraction method proposed for this work was not expensive and the flavonoids were obtained in large amounts, thus, this extraction method is being developed using pilot plant scale-up techniques and will soon be available to the industry at a low cost.

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Section: Resultsmentioning

confidence: 99%

An Environmentally Friendly Procedure to Obtain Flavonoids From Brazilian Citrus Waste

Bellete¹,

Ramin²,

Porto³

et al. 2018

J. Braz. Chem. Soc.

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“…Subsequent metabolomics studies revealed a primary accumulation of several hydroxycinnamates and certain selected flavonoids such as hesperidin, naringenin and quercetin (Cevallos-Cevallos et al, 2009;Hijaz et al, 2013). Although the specific role of these metabolites on bacterial growth and expansion has not been assessed, hesperidin accumulation in sweet orange has also been associated to defense mechanism against the bacterial pathogen Xylella fastidiosa (Soares et al, 2015).…”

Section: Phytoalexinsmentioning

confidence: 99%

Metabolomics of Disease Resistance in Crops

Arbona

Gómez-Cádenas²

2016

Current Issues in Molecular Biology

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Plants are continuously exposed to the attack of invasive microorganisms, such as fungi or bacteria, and also viruses. To fight these attackers, plants develop different metabolic and genetic responses whose final outcome is the production of toxic compounds that kill the pathogen or deter its growth or semiotic molecules that alert other individuals of the same plant species. These molecules are derived from the secondary metabolism and their production is induced upon detection of a pathogen-associated molecular pattern (PAMP). These PAMPs are different molecules that are perceived by the host cell triggering defense responses. PAMP-elicited compounds are highly diverse and specific of every plant species and can be divided into preformed metabolites or phytoanticipins that are converted into toxic molecules upon pathogen perception, and toxic metabolites or phytoalexins that are produced only upon pathogen attack. Moreover, plant volatile emissions are also modified in response to pathogen attack to alert neighboring individuals or to make plants less attractive to pathogen vector arthropods. Plant metabolite profiling techniques have allowed the identification of novel antimicrobial molecules that are induced upon elicitation. However, more studies are required to assess the specific function of metabolites or metabolite blends on plant-microbe interactions.

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“…Matrix‐assisted laser desorption/ionisation high–resolution mass spectrometry (MALDI–HRMS) imaging is the method of choice for acquisition of the spatial distribution and localisation of target molecules directly from tissue sections . MALDI–HRMS technique gives useful information on the distribution, biosynthesis, transport and storage of target molecules in plant tissues .…”

Section: Introductionmentioning

confidence: 99%

“…17,18 Matrix-assisted laser desorption/ionisation high-resolution mass spectrometry (MALDI-HRMS) imaging is the method of choice for acquisition of the spatial distribution and localisation of target molecules directly from tissue sections. [19][20][21] MALDI-HRMS technique gives useful information on the distribution, biosynthesis, transport and storage of target molecules in plant tissues. 22 For instance, spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species has been investigated using MALDI-HRMS imaging techniques.…”

Section: Introductionmentioning

confidence: 99%

MALDI–HRMS imaging and HPLC–HRESI–MSⁿ characterisation of kaurane diterpenes in the fruits of Xylopia aethiopica (Dunal) A. Rich (Annonaceae)

Kyekyeku

Asare–Nkansah

Bekoe

et al. 2019

Phytochemical Analysis

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Introduction Kaurane diterpenes, notably xylopic acid, have demonstrated important biological activities including analgesia, anti‐oxidant, antimicrobial and cytotoxicity. The fruits of Xylopia aethiopica have been reported to be a rich source of kaurane diterpenes. Objective An analytical approach for detailed imaging and characterisation of selected kaurane diterpenes was developed using matrix‐assisted laser desorption/ionisation high‐resolution mass spectrometry (MALDI‐HRMS) imaging techniques and high‐performance liquid chromatography‐high resolution electrospray ionisation‐tandem mass spectrometry (HPLC‐HRESI‐MSn) studies, respectively. Methods The images of the compounds were constructed based on selected ions from their HRESI–MS spectra. The matrix employed comprised a solution of α–cyano–4–hydroxycinnamic acid (HCCA) in acetonitrile–water with trifluoroacetic acid (TFA). HPLC–HRESI–MSn measurements were conducted on an LTQ–Orbitrap spectrometer equipped with a heated electrospray ionisation (HESI)–II source. Results The analytical strategy adopted showed the spatial distribution of the compounds in the fruits of X. aethiopica based on the dominant ions at m/z 301.2163 [M + H – HOCOCH3]+ and m/z 399.1932 [M + K]+ for xylopic acid, m/z 317.2111 [M + H]+ and m/z 355.1670 [M + K]+ for 15–oxo–ent–kaur–16–en–19–oic acid and m/z 303.2319 [M + H]+ for ent–kaur–16–en–19–oic acid. The fragmentation patterns of the compounds were proposed based on the HRESI–MSn measurements. Conclusions The study revealed the spatial variability, differential behaviours and specificity of the selected kaurane diterpenes in the fruit, seed and pericarp. The compounds under study were predominantly restricted to the pericarp of the fruit with trace amounts in the seed.

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Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry

Cited by 62 publications

References 28 publications

An Environmentally Friendly Procedure to Obtain Flavonoids From Brazilian Citrus Waste

An Environmentally Friendly Procedure to Obtain Flavonoids From Brazilian Citrus Waste

Metabolomics of Disease Resistance in Crops

MALDI–HRMS imaging and HPLC–HRESI–MSⁿ characterisation of kaurane diterpenes in the fruits of Xylopia aethiopica (Dunal) A. Rich (Annonaceae)

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Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry

Cited by 62 publications

References 28 publications

An Environmentally Friendly Procedure to Obtain Flavonoids From Brazilian Citrus Waste

An Environmentally Friendly Procedure to Obtain Flavonoids From Brazilian Citrus Waste

Metabolomics of Disease Resistance in Crops

MALDI–HRMS imaging and HPLC–HRESI–MSn characterisation of kaurane diterpenes in the fruits of Xylopia aethiopica (Dunal) A. Rich (Annonaceae)

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Product

Resources

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MALDI–HRMS imaging and HPLC–HRESI–MSⁿ characterisation of kaurane diterpenes in the fruits of Xylopia aethiopica (Dunal) A. Rich (Annonaceae)