Quantification and identification of particle-associated bacteria in unchlorinated drinking water from three treatment plants by cultivation-independent methods

Liu, G.; Ling, Fangqiong; Magic-Knezev, Aleksandra; Liu, W.T.; Verberk, J.Q.J.C.; Dijk, J. C. van

doi:10.1016/j.watres.2013.03.058

Cited by 61 publications

(38 citation statements)

References 54 publications

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“…Subsequently the number of bacteria was reduced, leading to a lower biodegradation rate. Liu et al (2013) found 25-50 cells attached to a single particle in unchlorinated drinking water just before its distribution from three treatment plants. They also reported the number of PAB ranged from 1.0 to 3.5 Â 10 3 mL À 1 cells demonstrating the potential of particles as attachment sites for bacteria.…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of multiple microcystins and cylindrospermopsin in clarifier sludge and a drinking water source: Effects of particulate attached bacteria and phycocyanin

Maghsoudi

Fortin

Greer

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Resultsmentioning

confidence: 99%

Biodegradation of multiple microcystins and cylindrospermopsin in clarifier sludge and a drinking water source: Effects of particulate attached bacteria and phycocyanin

Maghsoudi

Fortin

Greer

et al. 2015

Ecotoxicology and Environmental Safety

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“…It is important to mention that the lower biomass concentration measured in distributed water by TCC (in the present study) and ATP (in [5]) in the distal supply area can be caused by biomass enrichment in DWDS either by cells attached as biofilm on the pipe wall surface [31] or by cells associated with particulate matter such as suspended solids and loose deposits [31, 32]. Detailed studies on biomass in different phases in DWDS, such as pipe wall biofilm and loose deposits, are necessary before such a conclusion can be made.…”

Section: Resultsmentioning

confidence: 99%

Flow Cytometry Total Cell Counts: A Field Study Assessing Microbiological Water Quality and Growth in Unchlorinated Drinking Water Distribution Systems

Liu

Mark

Verberk

et al. 2013

BioMed Research International

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The objective of this study was to evaluate the application of flow cytometry total cell counts (TCCs) as a parameter to assess microbial growth in drinking water distribution systems and to determine the relationships between different parameters describing the biostability of treated water. A one-year sampling program was carried out in two distribution systems in The Netherlands. Results demonstrated that, in both systems, the biomass differences measured by ATP were not significant. TCC differences were also not significant in treatment plant 1, but decreased slightly in treatment plant 2. TCC values were found to be higher at temperatures above 15°C than at temperatures below 15°C. The correlation study of parameters describing biostability found no relationship among TCC, heterotrophic plate counts, and Aeromonas. Also no relationship was found between TCC and ATP. Some correlation was found between the subgroup of high nucleic acid content bacteria and ATP (R 2 = 0.63). Overall, the results demonstrated that TCC is a valuable parameter to assess the drinking water biological quality and regrowth; it can directly and sensitively quantify biomass, detect small changes, and can be used to determine the subgroup of active HNA bacteria that are related to ATP.

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“…complete masking by inclusion of bacteria in aggregates) cannot be ruled out from this study. Microbial attachment to surfaces is driven by complex multivariate factors including electrostatic interactions, van der Waals forces, hydrophobicity, surface tension and surface roughness [23,24,25] that are difficult to be simulated. Also the particle size is critical in protection of particle associated microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Effect of turbidity on water disinfection by chlorination with the emphasis on humic acids and chalk

Léziart

Rochere

Cheswick

et al. 2019

Environmental Technology

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Please refer to any applicable publisher terms of use. Highlights  Different turbidities have different effects on chlorine disinfection  Humic acids interfere with chlorination at ≥ 1NTU (2 mg/L)  Chalk does not interfere with chlorination up to 5 NTU  Both culture and flow cytometry lead to same conclusions 3 AbstractChlorine is globally the most widely used chemical for water disinfection. Whereas disinfection efficiency is well known to depend on water pH and temperature, the effect of turbidity is less well studied. Although turbidity is measured online in most drinking water works and most countries where regulations exist have set limits of < 1 NTU for water leaving the works, the composition of turbidity is typically unknown. Given the heterogeneous nature of substances contributing to turbidity, the aim of this work was to study the effect of selected compounds on chlorination efficacy. The effect of humic acids and chalk on the inactivation of the indicator bacteria Escherichia coli and Enterococcus faecalis was assessed at neutral pH at different turbidity levels using both plate counting and flow cytometry in combination with membrane integrity staining. For humic acids, a turbidity of 1 NTU (corresponding to 2 mg/L) was identified as a critical threshold, which when exceeded was found to have a negative impact on chlorine disinfection. Chalk, on the other hand, had no measurable impact up to 5 NTU. The observation applied to both bacterial species with identical conclusions from the two diagnostic methods. Results corroborate that different turbidity causing substances affect chlorination efficiency to very different extents with chlorine demand by organic material probably being the most important determinant. In case of turbidities > 1 NTU, turbidity measurement benefits from the consideration of the organic content as mere NTU values do not allow predicting an impact on chlorination efficiency.

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Quantification and identification of particle-associated bacteria in unchlorinated drinking water from three treatment plants by cultivation-independent methods

Cited by 61 publications

References 54 publications

Biodegradation of multiple microcystins and cylindrospermopsin in clarifier sludge and a drinking water source: Effects of particulate attached bacteria and phycocyanin

Biodegradation of multiple microcystins and cylindrospermopsin in clarifier sludge and a drinking water source: Effects of particulate attached bacteria and phycocyanin

Flow Cytometry Total Cell Counts: A Field Study Assessing Microbiological Water Quality and Growth in Unchlorinated Drinking Water Distribution Systems

Effect of turbidity on water disinfection by chlorination with the emphasis on humic acids and chalk

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