QuantiFERON TB-2G test for patients with active tuberculosis stratified by age groups

Kobashi, Yoshihiro; Mouri, Keiji; Miyashita, Naoyuki; Okimoto, Niro; Matsushima, Toshiharu; Kageoka, Takeshi; Oka, M.

doi:10.3109/00365540903186215

Cited by 25 publications

(26 citation statements)

References 15 publications

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“…23 In our study, the T-SPOT.TB test showed the highest concordance rate in pulmonary TB patients in the 21e30 age group (97%), in accordance with previous reports indicating that the QFT-G and QFT-IT tests have higher sensitivity in the 20e29 age group among Japanese and Korean TB patients (92%, 93% and 89.5%). 22,35,37 Taken together, these results confirm the detrimental impact of increased age on the sensitivity of the T-SPOT.TB test. It has previously been suggested that lymphocytopenia and decreased function of T lymphocytes cells may be the two causative reasons of decreased sensitivity in IGRA tests.…”

Section: Relationship Between Ifn-g Production and The Risk Factors Isupporting

confidence: 83%

“…Older age (OR 2.42, 95% CI 1.09e5. 35) was the only independent risk factor for false-negative T-SPOT.TB results for EPTB as determined by multivariate logistic regression analysis ( Table 4).…”

Section: Risk Factors For False-negative T-spottb Resultsmentioning

confidence: 99%

“…Liao et al did not analyze the IFN-g production and the concordance rate of T-SPOT.TB by age, possibly because of the lower number of false-negative T-SPOT.TB results (only 10 patients) in their study. Increased age has also been validated as a risk factor for false-negative results in the OFT-G and QFT-IT tests in patients with active TB, 22,35,36 and in smear-positive pulmonary TB patients. 23 In our study, the T-SPOT.TB test showed the highest concordance rate in pulmonary TB patients in the 21e30 age group (97%), in accordance with previous reports indicating that the QFT-G and QFT-IT tests have higher sensitivity in the 20e29 age group among Japanese and Korean TB patients (92%, 93% and 89.5%).…”

Section: Relationship Between Ifn-g Production and The Risk Factors Imentioning

confidence: 98%

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Risk factors for false-negative T-SPOT.TB assay results in patients with pulmonary and extra-pulmonary TB

Pan

Jia

Liu

et al. 2015

Journal of Infection

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Section: Relationship Between Ifn-g Production and The Risk Factors Isupporting

confidence: 83%

Section: Risk Factors For False-negative T-spottb Resultsmentioning

confidence: 99%

Section: Relationship Between Ifn-g Production and The Risk Factors Imentioning

confidence: 98%

See 1 more Smart Citation

Risk factors for false-negative T-SPOT.TB assay results in patients with pulmonary and extra-pulmonary TB

Pan

Jia

Liu

et al. 2015

Journal of Infection

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“…TB was lower in the elderly than in younger individuals with suspected active TB. [111213] An increased age may be associated with low immunity, poor nutritional status, and increased comorbidity. The quantity and function of T-cells declines with age, resulting in decreased specificity of the antigen−antibody reaction, and thus decreased sensitivity of the T-SPOT ® .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Significance of Mycobacterium tuberculosis T-cell Assays for Active Tuberculosis

Xuan

Lü

Wang

et al. 2017

Chinese Medical Journal

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Background:Active tuberculosis (TB) with negative results of sputum smear is difficult to be identified. Till now, there is no effective and noninvasive diagnostic method. This study evaluated the diagnostic power of Mycobacterium tuberculosis T-cell (T.SPOT®.TB) assays for active TB.Methods:We retrospectively screened 450 suspected TB patients that were hospitalized in the Respiratory Department of Henan Province People's Hospital from June 2015 to June 2016. The patients were divided into the active, previous, and non-TB groups according to their final diagnosis. We evaluated the diagnostic value of the T-SPOT®.TB assay by constructing receiver operating characteristic (ROC) curves and calculating the optimal diagnostic cutoff value. In addition, we compared the levels of A antigen (ESAT-6) and B antigen (CFP-10) in active TB.Results:The sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio of T-SPOT®.TB for active TB were 89.78%, 63.16%, 0.56, 0.92, 2.47, and 0.16, respectively. For active TB, the area under the ROC curve (AUC) of the A antigen (0.89) was higher than that of the B antigen (0.86). The AUC of the A antigen for active TB was largest at a cutoff value of 13.5 spot-forming cells (SFCs) per 2.5 × 105 peripheral blood mononuclear cells (PBMCs). The AUC of the A and B antigens was 0.60 and 0.58 for previous TB. The levels of A and B antigen in the active TB group were significantly different from those in the previous- and non-TB groups (A antigen: χ2 = 105.41, P < 0.01 and B antigen: χ2 = 91.03, P < 0.01; A antigen: χ2 = 12.99, P < 0.01 and B antigen: χ2 = 8.56, P < 0.01, respectively). There were no significant differences in the levels of A and B antigens between the non-TB group and previous TB group (A antigen: χ2 = 1.07, Pw> 0.05 and B antigen: χ2 = 0.77, P> 0.05).Conclusions:T-SPOT®.TB has high sensitivity and specificity for the diagnosis of active TB at a cutoff value of 13.5 SFCs per 2.5 × 105 PBMCs and is not influenced by previous TB.

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“…QFT results are not affected by former Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination or immune reactivity to nontuberculous mycobacteria. Several studies have demonstrated its usefulness in supporting the diagnosis of pulmonary and extrapulmonary TB (5)(6)(7)(8). Making the correct diagnosis has important therapeutic implications, since TRU must be treated with antitubercular therapy (ATT) to prevent vision loss (1-3, 9, 10).…”

mentioning

confidence: 99%

QuantiFERON-TB Gold Assay on Plasma for Confirmation of Presumed Tuberculosis-Related Uveitis

et al. 2016

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bThe QuantiFERON-TB Gold assay was used to measure interferon gamma levels in plasma from 4 patients with presumed tuberculosis-related uveitis before, during, and after antitubercular therapy. After treatment, all patients showed clinical improvement. The concentrations showed a reversion to an absence of interferon gamma in one case, decreased in two cases, and remained stable in one case. These results suggest that the QuantiFERON assay may be useful for tuberculosis-related uveitis diagnosis and follow-up.T uberculosis (TB)-related uveitis (TRU) is an uncommon form of extrapulmonary TB. TRU may have multiple clinical presentations, including anterior, intermediate, and posterior uveitis, panuveitis, retinitis and retinal vasculitis, neuroretinitis and optic neuropathy, and endophthalmitis (1). The lack of uniform criteria for the diagnosis of TRU accounts for the confusion concerning its management and the absence of reliable epidemiological data (1). The microbiological diagnosis of TRU is difficult, because most patients have no evidence of Mycobacterium tuberculosis in their ocular specimens. Indeed, in patients with TRU, tests detecting M. tuberculosis growth in cultures, acid-fast bacilli on smears, or M. tuberculosis DNA using PCR often yield no results. This may be due to a low level of inoculum or, more commonly, to immunedriven inflammation in the absence of active disease (latent infection). The lack of microbiological confirmation for presumed TRU makes indirect testing with QuantiFERON-TB Gold (QFT) the main support for this diagnosis (2,3). QFT is an interferon gamma (IFN-␥) release assay (IGRA) that measures the concentration of IFN-␥ obtained in response to the stimulation of whole blood with ESAT-6-, CFP-10-, and TB7.7-specific M. tuberculosis antigens (4). QFT results are not affected by former Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination or immune reactivity to nontuberculous mycobacteria. Several studies have demonstrated its usefulness in supporting the diagnosis of pulmonary and extrapulmonary TB (5-8). Making the correct diagnosis has important therapeutic implications, since TRU must be treated with antitubercular therapy (ATT) to prevent vision loss (1-3, 9, 10). From this small case series, we present the results of QFT and ATT use in four immunocompetent patients with presumed TRU from an area (Sardinia, Italy) with low TB incidence.Approval from the local ethics committee/institutional review board was obtained. Three men and one woman with presumed TRU were examined at the Department of Surgical, Microsurgical, and Medical Sciences, Ophthalmology Unit, University of Sassari, Sassari, Italy, between September 2014 and September 2015. None had been vaccinated with BCG, and two reported an old close contact with active TB patients. The ophthalmic manifestations included cyclitis, vitreous inflammation, and vasculitis (patients 1 and 2), cyclitis and vitreous inflammation (patient 3), and uveopapillitis (patient 4). Overall, bilateral panuveitis was identified in thr...

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QuantiFERON TB-2G test for patients with active tuberculosis stratified by age groups

Cited by 25 publications

References 15 publications

Risk factors for false-negative T-SPOT.TB assay results in patients with pulmonary and extra-pulmonary TB

Risk factors for false-negative T-SPOT.TB assay results in patients with pulmonary and extra-pulmonary TB

Diagnostic Significance of Mycobacterium tuberculosis T-cell Assays for Active Tuberculosis

QuantiFERON-TB Gold Assay on Plasma for Confirmation of Presumed Tuberculosis-Related Uveitis

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