Quality tools and techniques

Binding affinities to rat brain synaptosomes – synthesis of biotinylated analogues of Substance P

“…Indeed, [a-biotinyl-Lys3] SP-(3-1 l), 10, which exhibits the same biological activity on the guinea-pig ileum bioassay as that of SP, is a weaker competitor (0.05-fold that of SP) in the central nervous system and has an inhibitory potency similar t o the nonapeptide SP- (3)(4)(5)(6)(7)(8)(9)(10)(11). The N-a-amino group from arginine is not essential for the binding to these receptors, since [a-biotinyl-Arg'] SP, 12, is almost as active as SP.…”

“…In the central nervous systeni we observe a net decrease in the inhibitory potencies of these con~plexes. Thus, the affinities of the complexes with I 0 and 12 with avidin reduce the '2s1-BHSP specific binding with 0.013 and 0.05-fold the potency of SP, corresponding t o the affinities of SP-(4-1 1) and SP- (3)(4)(5)(6)(7)(8)(9)(10)(11) respectively. This implies that, at the least, the amino acid linked t o biotin is no longer available for receptor recognition.…”

“…methanol); RF0.47; Rf0.78; n.ni.r.N-a-Biotinyl-N-~-beiizqtl~~xycarbonyl-t~-lvs~~l-r~ proline methyl ester,9. To a solution of Lys (Cbz)-Pro-OMe (982 mg, 2 niniol) in 10 niL DMF was added N-methylniorpholine until pH as described to yield the free acid which was coupled by the mixed anhydride method to SP-(5)(6)(7)(8)(9)(10)(11) to yield [N-cu-Boc-N-e-Lys3(Cbz)] SP-(3-11) 540 mg, which was used without further purification. The N-a-Boc protecting group was removed as described and the methyl ester of N-a-biotinyl-Arg (N02)-Pro-OMe, 7, was hydrolyzed to yield the corresponding free acid, RF0.29; n.m.r.…”

Business Process Management: A Life Cycle Approach

“…In other words, the PDCA's objective is to plan to do something, manufacture or do it, check it for meeting requirements, and correct the process to maintain the acceptable output performance (Gupta, 2006). The cause and effect diagram graphically exhibits the relationships between a particular outcome (effect) and all of the identified possible causes that contribute to that outcome (McCormick, 2002). …”