Quality parameters, fatty acid profiling and estimation of umbelliferone in grahaṇimihira tailam: An ayurvedic oil preparation

Abstract

Background:Grahaṇimihira tailam is an unexplored ayurvedic oil preparation which consists of 34 ingredients. The efficacy of this traditional ayurvedic medicine is undisputable. Proper clinical standardization of this formulation will go a long way in securing greater recognition for it. The main objective of this study was to develop standardization parameters for the formulation in a multidisciplinary way.Materials and Methods:A simple and efficient method for the quantification of umbelliferone by high performance thin layer chromatography was developed and validated. Presence of the major fatty acids and their percentage were assessed by using gas chromatography-mass spectrometry (GC-MS). Various physio-chemical parameters, microbiological load, aflatoxins and mineral oil were also evaluated. Spread plate method was used for checking microbial contamination.Results:The results were validated as per standard protocols. Quantitative estimation revealed the percentage of umbelliferone to be in the range of 0.88-0.98 (w/w). GC-MS analysis of sample led to the identification of 14 fatty acids, in which linoleic acid was obtained as the major fatty acid. Microbes, aflatoxins and mineral oils were found to be absent in the tailam.Conclusion:The results which give the quantitative estimates of various physico-chemical parameters can be adopted to establish new standards for analysis of batch-to-batch variation and this data will facilitate shelf life studies in the future.

Standardization and preliminary characterization of an ayurvedic stress-relieving head massage oil of Nardostachys jatamansi DC

“…Metabolites viz., Calarene, Vardiflorene, α- Panasinsen, α- Santalene, γ-Himachelene, Jatamansone, Ionol 4, 2,2,7,7- Tetramethyl tricyclo[6,2,1,0 (1,6)] undec-4-ene-3-one, Epiglobulol, and Resibufogenin were identified in volatile oil of NJ [ 15 ]. GC-MS has been widely used for the characterization of Ayurvedic and Siddha formulations such as oils/ghee [ 16 , 17 ], guggulu ( Commiphora wightii resin formulations) [ 18 ], and asava-arishta (hydroalcoholic formulations) [ 19 ]. The importance of medicated oils as prepared by Ayurvedic methods has been previously studied indicating high stability and recognizable changes in the finished products containing specific chromatographic changes [ 20 ].…”

Bacterial contamination in Thai Ayurveda products

“…Overall, 101 Thai Ayurveda product samples were collected and studied and it was found that 57.4 % had bacterial contamination over the acceptable Thailand pharmaceutical standards, TP Supplement 2005. [ 3 ] According to these standards, acceptable level includes all of the following criteria: (a) Total viable aerobic count ≤5.0 × 105/g or ml. (b) Yeast and molds count ≤5.0 × 103/g or ml.…”

“…(g) No Clostridium spp./10 g or ml. [ 3 ] Compared to the survey of the locally produced products in India, of which null prevalence of contamination was reported,[ 4 ] the high contamination rate in Thailand is a serious problem. Hence, there is an urgent need for quality management of the Ayurveda pharmaceutical product manufacturing process of the local district hospitals.…”