Background: Youth friendly reproductive health services are designed to entertain the unique needs of youth. Nevertheless, in developing countries like Ethiopia, knowledge, attitude and utilization of these services is very limited. This study was designed to assess the knowledge, attitude and the extent of utilization of youth friendly reproductive health services (YFRHS) and associated factors among students of Mizan-Tepi University, Southwestern, Ethiopia. Method: A public University-based cross-sectional study was conducted between October and January 2019. Simple random sampling technique was used to select participants from target population. Descriptive analysis, Chi-square test and logistic regression model were employed to identify the factors associated with YFRHS. Result: The results of descriptive analysis showed that about 55.4% of participants had no knowledge, 40.2% had negative attitudes and 61.2% had not practiced YFRHS. Majority 258(60.30%) of the respondents were females and 209(48.80%) were in the age group of 21-22 years. Most of the respondents lack basic information and knowledge, and have no any YFRHS in their surroundings 220(51.4%). Out of the 428 respondents, 57.9%, 59.3%, and 36.4% had never been practiced services related to VCT, family planning and contraception and treatment of sexually transmitted infections (STIs), respectively. The multivariable binary logistic regression result revealed that sex, age, religion, mother’s and father’s educational background, availability of YFRHS in the area and source of information had statistical significant effect on the knowledge, attitude and practices of students toward YFRHS. Male students were more likely to have knowledge and practicing experiences (OR = 1.847, p-value =0.015) and (OR=1.821, p-value = 0.017) respectively, however they had less likely positive attitudes (OR=0.519, p-value = 0.010) compared to their counterpart females. Students from primary and above educated mothers and fathers were more likely to have knowledge, attitude and practicing experience compared to students from uneducated mothers and fathers. Conclusion: The overall utilization, attitude and knowledge of YFRHS among study respondents’ are lower and therefore, further efforts like availability of YFRHS providers and improvement of facility, and education related to the service to all young groups and allocation of appropriate time of services is essential.