Background: Improving the quality of nurses’ working life is challenging for healthcareorganizations because of its effect on the staff, organizations, and customers. Thedevelopment of supportive environments will enhance the quality of nursing work lifein organizations. However, the association between perceived organizational supportand the quality of working life among nurses was limited information.Aim: This study aims to assess the relationship between perceived organizationalsupport and the quality of nurses’ work life at a King Fahad Hospital Jeddah, SaudiArabia.Methodology: This current study was conducted at King Fahad Hospital, Jeddah,Saudi Arabia. A descriptive correlational design was used. A convenience samplingtechnique was implemented to gather the needed data using a self-report questionnaire.Data was collected using the Survey of Perceived Organizational Support and Brook’Quality of Nursing Work Life Survey. In addition, a demographic questionnaire wasdeveloped by the researcher.Results: About 80.6% of staff nurses showed a moderate POS level, and 63.6% of staffnurses experienced an average level of QNWL. The Pearson correlation coefficientsshowed a statistically significant correlation between POS and overall QNWL (0.541).The correlations between POS and the sub-scales of QNWL came in the range (0.367– 0.521). Also, it found that non-Saudi nurses exhibited higher POS and QNWL levelscompared to Saudi nurses.Conclusion and Recommendation: The study found that perceived organizationalsupport is positively related to QNWL, which means that the higher nurses’ perceivedorganizational support will improve the nurses’ quality of working life. These findingsshould be used by nursing administrations and health policymakers to develop a seriesof organizational support that can improve the quality of working life amongnurses. Future research, further comparative research is needed to examine POS andits relation to QNWL among different healthcare organizations.Key words: Perceived Organizational Support–Quality of Nurses’ Work Life–Qualityof Nursing Work Life–Staff nurses