Quality of pooled platelet concentrates prepared from buffy coats and stored in an additive solution after filtration

Abstract

Platelet concentrates prepared from buffy coat were pooled and stored for 6 days after removal of leukocytes by filtration. The platelets were stored in plasma or in an additive solution, Plasmalyte-A. In vitro platelet function was better preserved using Plasmalyte-A than plasma with regard to osmotic reversal and aggregation. No significant differences for the release of platelet markers beta-thromboglobulin, platelet factor 4, or lactate dehydrogenase pre- and post-filtration and storage in plasma or Plasmalyte-A was observed. Expression of the surface membrane glycoproteins Ib, Ia/IIa, IIb/IIIa, and IV measured by flow cytometry after binding of monoclonal antibodies did not change during storage. The expression of activation-dependent alpha-granula glycoprotein GMP140, the thrombospondin, and the glycoprotein 53 from the lysosomal granules was not different between platelet pools stored in plasma or in Plasmalyte-A. The in vitro quality of platelets stored as pools is comparable for plasma and the additive solution Plasmalyte-A.

The influence of platelet additive solutions on cytokine levels and complement activation in platelet concentrates during storage

“…As PASIII is identical to PASII, except for the addition of phosphate to the medium, the similarity of results between our study and that of de Wildt‐Eggen et al [13] is not unexpected. It is probable that the degree of platelet activation in PC is related to the composition of the PAS, as cell‐surface CD62P levels in PC stored in a different PAS – Plasmalyte – are not significantly different to those in plasma [29]. The percentage of platelets expressing CD62P was higher in all units stored in PAS compared with plasma, the highest levels being seen with standard PASIII.…”

The storage lesion of platelets: ultrastructural and functional aspects

“…Several studies on the use of synthetic storage media confirm the large benefit of acetate in the maintenance of platelet quality during storage [27,28,47,48,58,72], but a minimum plasma carryover of at least 15% seems to be essential for preservation of platelet integrity. With a lower plasma portion, the remaining buffer capacity and glucose levels are not able to allow a successful storage of platelets [46,81], and the requirements for the synthetic medium become very rigorous [58].…”

“…When stored in autologous plasma and in containers with sufficient gas permeability, these two metabolic parameters are stable for at least 5 days, but when balanced salt solutions without adequate fuels or buffer capacity are substituted for the blood plasma, metabolic problems may occur to a variable degree. Several studies have shown the benefit of additive solutions for the storage of PC [28,47,48] provided that a minimum plasma carryover of 15-20% is given. With the introduction of acetatecontaining additive solutions, the energy supply as well as the stability of the pH within the PC were markedly improved [27-29, 58, 81].…”