Quality of packed red blood cells and platelet concentrates collected by multicomponent collection using the MCS plus device

Abstract

The demand for blood components is constantly increasing, while the exclusion criteria for donors are strengthened in order to reach maximal safety for donors and patients. To counterbalance reduced availability of volunteers, multicomponent collections (MCC) is an attractive approach to produce more than one component during a single apheresis procedure from one donor, such as packed red blood cells (PRBCs) and platelet concentrates (PCs). Further, the exposures of patients to a limited number of donors reduces the possibility of alloimmunization and transfusion-related diseases. We measured the quality of PRBCs and PCs obtained by MCC, using the MCS+ device with the LDPRBC program, Revision B, and compared them with the quality of manually collected PRBCs and PCs collected with the Revision C2 of the MCS+. We found higher pH levels and lower hemolysis assessed by means of fHb and K+ in the supernatant of PRBCs over the whole storage period of 42 days in MCC-derived PRBCs. The functional metabolism assessed by intracellular ATP was higher in PRBCs collected by MCC than in manually collected units. Furthermore, PCs obtained during MCC showed an increase in p-selectin expression on day 5 of storage compared to PCs collected with the Revision C2 of the MCS+. The p-selectin expression on MCC platelets was within the range of p-selectin expression found in PCs obtained by other apheresis devices. These results indicate less storage lesion in MCC-derived PRBCs compared to manually collected units and no compromise in the quality of MCC PCs obtained in the same apheresis procedure.

Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

“…A possible explanation for the increased haemolysis could be the shear stress due to the multiple centrifugation steps. During MNC separation, the RBCs were exposed to low centrifugal forces of 136 g and 930 g compared to 1270 g used during the processing of homologous PRBCs from whole blood but the centrifugation time of approximately 62 min was about six times longer than during the conventional process of whole blood derived PRBCs (P < 0AE05), confirming previous results that showed the impact of mechanical forces on the extent of red cell damage [19].Potassium, lactate and pH values, in contrast, remained within the ranges of freshly collected RBCs during the whole production process [11,20]. To maintain a physiological ion gradient in RBCs, the function of the Na+ ⁄ K+ pump, which is powered by intracellular purines, is essential.…”

“…Here, we included 19 patients with myocardial infarction enclosed in the MYSTAR study, who received enriched CD34 + cells in a volume of approximately 4 ml intramyocardially [16,18]. Despite depletion of haemolytic supernatant from the remaining RBC fraction after BM procession, the recovered RBCs showed higher LDH and fHb levels as compared with unprocessed BM or homologous packed RBC concentrates (PRBCs) at the end of shelf life at day 42 [11,19,20]. In two units of recovered RBCs, haemolysis was even above the threshold of 0AE8% [14].…”

“…Potassium, lactate and pH values, in contrast, remained within the ranges of freshly collected RBCs during the whole production process [11,20]. To maintain a physiological ion gradient in RBCs, the function of the Na+ ⁄ K+ pump, which is powered by intracellular purines, is essential.…”

“…When paediatric BM donors were investigated and BM-RBCs were separated by density gradient centrifugation using Ficoll-Hypaque, similar results were In a prospective analysis, we assessed the extent of cell lesions occurring during manipulation by measuring the release of lactate dehydrogenase (LDH), free haemoglobin (fHb), potassium (K+) and lactate at each manipulation step. Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate marker for RBC function [11][12][13]. …”

“…Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate marker for RBC function [11][12][13].…”