Quality of IVM ovarian tissue oocytes: impact of clinical, demographic, and laboratory factors

Abstract

Purpose To determine how clinical, demographic, and laboratory characteristics influence ovarian tissue oocyte quality. Methods Immature cumulus-oocyte complexes were isolated from removed ovaries and cultured for 48–52 h in either monophasic standard or biphasic CAPA media for fertility preservation. A total of 355 MII oocytes from 53 patients were described for intracytoplasmic and extracytoplasmic anomalies. Multiple clinical, laboratory, and demographic characteristics were analyzed. Statistically significant differences between independent groups in qualitative variables were identified using Pearson’s χ2 and Fisher’s exact tests. The diagnostic value of quantitative variables was assessed using the ROC curve analysis. Factors associated with the development of dysmorphism, taking patient age into account, were identified using the binary logistic regression analysis. Results Dysmorphisms were observed in 245 oocytes (69.0%), with a median number of dysmorphisms of 2. Oocyte dysmorphisms were found to be 2.211 times more likely to be detected in patients with ovarian cancer, while the presence of dark-colored cytoplasm was associated with gynecologic surgery in the anamnesis (p = 0.002; OR 16.652; 95% CI, 1.977–140.237; Cramer’s V 0.187). Small polar bodies developed 2.717 times more often (95% CI, 1.195–6.18) in patients older than 35. In the case of ovarian transportation on ice at 4 ℃, the chances of development of cytoplasmic granularity increased 2.569 times (95% CI, 1.301–5.179). The use of biphasic CAPA IVM media contributed to a decrease in the probability of large polar body formation (p = 0.034) compared to the standard monophasic IVM media. Conclusions Both patients’ characteristics and laboratory parameters have an impact on the quality of IVM ovarian tissue oocytes.