Quality of Fresh, Cooled, and Frozen Semen From Stallions Supplemented with Antioxidants and Fatty Acids

Freitas, Mariane Leão; Bouéres, Cristiano Silva; Pignataro, Tatiana Almeida; Oliveira, Francisco José Gonçalves de; Viu, Marco Antônio de Oliveira; Oliveira, Rodrigo Arruda de

doi:10.1016/j.jevs.2016.07.003

Cited by 19 publications

(18 citation statements)

References 33 publications

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“…With decreased sperm motility, fertilization potential is also reduced. Therefore, supplementation with fatty acids should be combined with antioxidants to effectively improve the integrity of the sperm membrane (SCHMID-LAUSIGK; AURICH, 2014;FREITAS et al, 2016). Although we used a formulation that contained only omegas and observed higher-quality fresh sperm post-supplementation, there was no improvement in the results of the frozen/thawed samples.…”

Section: Discussionmentioning

confidence: 93%

“…Additionally, the authors concluded that increased sperm motility is related to the use of antioxidants, while the improvement in membrane integrity was attributed mainly to the higher concentration of polyunsaturated fatty acids associated with antioxidants. Thus, in stallions, the isolated use of omegas did not result in large effects on sperm quality (FREITAS et al, 2016). These effects were attributed only to antioxidant supplementation (SCHMID-LAUSIGK; AURICH, 2014).…”

Section: Discussionmentioning

confidence: 98%

“…For stallions, in addition to vitamin E, supplementation with L-carnitine and selenium in combination with omega-3 and -6 resulted in increased motility and integrity of the acrosome and plasma membranes of fresh and frozen/thawed semen (FREITAS et al 2016). Additionally, the authors concluded that increased sperm motility is related to the use of antioxidants, while the improvement in membrane integrity was attributed mainly to the higher concentration of polyunsaturated fatty acids associated with antioxidants.…”

Section: Discussionmentioning

confidence: 99%

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Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Rodrigues

Ruiz

Nardo

et al. 2017

SCA

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For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/ thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1 st -5 th week) or pre-supplementation; M2 (6 th -9 th week) and M3 (10 th -13 th week) or during supplementation; and M4 (14 th -17 th week) or postsupplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen. Key words: Canine. Diet-supplemented. Polyunsaturated-fatty-acid. Sperm. ResumoOs ácidos graxos têm sido recomendados há anos como suplemento dietético para a melhora do pelo canino. Na área de reprodução animal, a suplementação com ômega tem sido usada para aumentar a eficiência reprodutiva e a taxa de concepção em muitas espécies, mas poucos estudos foram conduzidos em cães. Então, o objetivo deste estudo foi avaliar os efeitos da suplementação dietética diária com ômega-3 e -6 sobre a qualidade do sêmen fresco e congelado de cães. de 7 cães e avaliados quanto a motilidade espermática, vigor, concentração e morfologia. O estudo foi dividido de acordo com os períodos de suplementação oral com omegas-3 e -6, no qual M1 (1ª-5ª semana) foi considerado o período pré-suplementação, M2 (6ª-9ª semana) e M3 (10ª-13ª semana) período de suplementação e M4 (14ª-17ª semana) pós-suplementação, totalizando 17 semanas de estudo (119 ejaculados). Após a análise, o sêmen foi congelado e reavaliado imediatamente e 30 minutos após a descongelação (mantidas a 37º C). A suplementação resultou em aumento da motilidade espermática, vigor e concentração do sêmen fresco; no entanto, não houve influência na congelabilidade do sêmen. Além disso, não houve aumento da motilidade dos espermatozoides após a suplementação, quando as células descongeladas foram mantidas a 37° C, durante 30 minutos. Concluiu-se que a suplementação dietética com omegas-3 e -6, durante 4 a 8 semanas melhora os parâmetros do sêmen fresco de cães, embora não há nenhum efeito sobre a qualidade espermática após a congelação. Palavras-chave: Ácido-graxo-poli-insaturado. Canino. ...

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Rodrigues

Ruiz

Nardo

et al. 2017

SCA

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“…The quantity of DHA in the human sperm membrane has been associated with higher motility and concentration, as well as changes in morphology (González‐Ravina et al, ; Gulaya et al, ; Safarinejad, Hosseini, Dadkhah, & Asgari, ; Tavilani et al, ). In other animal species, there are reports of improved acrosome and sperm membrane integrity in horses (Freitas et al, ); elevation of motility, viability and membrane integrity in roosters (Safari Asl, Shariatmadari, Sharafi, Karimi Torshizi, & Shahverdi, ); a significant increase in the semen volume and total number of spermatozoa in pigs and dogs (Murphy et al, ; da Rocha, Cunha, Ederli, Albernaz, & Quirino, ; Tufarelli et al, ); and improved sperm quality in water buffaloes (Tran et al, ) and sheep (Esmaeili, Shahverdi, Alizadeh, Alipour, & Chehrazi, ). However, the use of PUFAs as dietary supplements to increase the quality of semen or treat testicular degeneration in dogs has not yet been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Residual effect after salmon oil supplementation on semen quality and serum levels of testosterone in dogs

Santos

Milani

Zucchini

et al. 2019

Reprod Domestic Animals

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The objective of present study was to evaluate the effects of oral supplementation of salmon oil on seminal parameters and testosterone serum levels in dogs, following also the residual effects for 60 days after treatment. Nine healthy male dogs with proven fertility, weighing between 10 and 36 kg, ageing from 2 to 11 years, of different breeds, fed diets supplemented with salmon oil at the manufacturer's recommended dosage. The parameters measured were sperm volume, motility, vigour, normal morphology and concentration, live/dead ratio, membrane viability by means of HOST test and serum testosterone levels. Evaluations occurred at baseline (D0), after 90 days of supplementation (D90) and at the end of the experiment, 60 days after supplementation cessation (D150). Results (mean ± SD) obtained at time D0, D90 and D150 were as follows: motility of 76.66% ± 13.7, 92.77 ± 4.41 and 93.0 ± 7.90 (p = .001); normal spermatozoa of 69.11% ± 24.90, 90.00% ± 5.15 and 80.66 ± 16.04 (p = .05); live/dead (%) from 64.44 ± 22.86 to 85.33 ± 8.41 (p = .001); and spermatozoa (%) with integral membrane in the membrane integrity (HOST) test ranging from 76.44 ± 20.74 to 91.22 ± 4.68 (p = .05). Serum levels of testosterone (ng/ml) increased from 5.50 ± 1.13 to 8.84 ± 1.13 at D90 (p = .003) and decreased after 2 months (D150) to 5.13 ± 1.13. In conclusion, a 90‐day supplementation with salmon oil had a positive influence on semen quality and serum testosterone levels. The supplementation of omegas 3 and 6 at the ratio of 10:1 for 90 days determined an increase in concentration and motility of the sperm, and these effects were maintained for 60 days, with the only exception of testosterone levels.

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“…These fatty acids are essential for the structure, function and integrity of the plasma membrane (Colenbrander et al, 1992). Thus, maintenance of the membrane is essential for the continuous cellular viability (Grady et al, 2009;Freitas et al, 2016).…”

Section: Omega-3mentioning

confidence: 99%

Nutraceutical in male reproduction

Freitas¹,

Oliveira

2018

Braz. J. Vet. Med.

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In order to obtain a successful outcome in artificial insemination programs, a few precautions must be taken with regard to male breeders. It is established that environmental factors can change the hormonal secretion, the cell differentiation that occurs in the testicles, as well as sperm maturation and transport in the epididymis. In view of adverse nutritional factors, reproductive organs can present degeneration and disorders in different degrees and intensities, affecting the animal fertility. The term nutraceutical describes products derived from foods, which may provide additional health benefits, beyond the basic value found in diets. Among the main nutraceuticals used in male reproduction, there are omega-3, arginine, B-complex vitamins, L-carnitine, β-carotene and antioxidants. The aim of this paper is to present the main substances used as nutraceuticals, in order to improve semen quality, obtaining most favorable outcomes of stallions in the use of natural breeding or artificial insemination.Keywords: artificial insemination, fertility, nutrition, semen, spermatogenesis.resumo Para um sucesso em programas de inseminação artificial, algumas precauções devem ser tomadas em relação aos reprodutores machos. Sabe-se que fatores ambientais podem alterar a secreção hormonal, a diferenciação celular que ocorre nos testículos, assim como o transporte e a maturação espermática no epidídimo. Um déficit nutricional pode levar a degeneração e distúrbios dos órgãos reprodutivos em diferentes graus e intensidade, afetando a fertilidade. O termo nutracêutico descreve produtos derivados dos alimentos, que podem fornecer benefícios adicionais a saúde, além dos valores básicos encontrados na dieta. Entre os vários nutracêuticos utilizados na reprodução, temos o ômega-3, arginina, vitaminas do complexo B, L-carnitina, β-caroteno e antioxidantes. Objetivou-se com essa revisão apresentar as principais substâncias utilizadas como nutracêuticos, com a finalidade de melhorar a qualidade seminal, obtendo resultados favoráveis dos garanhões com a monta natural ou a inseminação artificial.Palavras-chave: inseminação artificial, fertilidade, nutrição, sêmen, espermatogênese.

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Quality of Fresh, Cooled, and Frozen Semen From Stallions Supplemented with Antioxidants and Fatty Acids

Cited by 19 publications

References 33 publications

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

Residual effect after salmon oil supplementation on semen quality and serum levels of testosterone in dogs

Nutraceutical in male reproduction

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