Quality of bull spermatozoa after preparation by single-layer centrifugation

Goodla, Lavanya; Morrell, Jane M.; Yusnizar, Yulnawati; Stålhammar, H.; Johannisson, Anders

doi:10.3168/jds.2013-7607

Cited by 45 publications

(68 citation statements)

References 32 publications

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“…Nevertheless, it must be highlighted that DNA fragmentation observed was very low and within the normal range described previously in other studies (1.5-5.0%) [34][35][36]. This result contrasts significantly with previous studies using other sperm pretreatments agents, such as DTT, which produced massive chromosome aberrations and high DNA fragmentation in mouse and bovine sperm [14,22].…”

Section: Discussioncontrasting

confidence: 82%

Improved preimplantation development of bovine ICSI embryos generated with spermatozoa pretreated with membrane-destabilizing agents lysolecithin and Triton X-100

et al. 2016

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Section: Discussioncontrasting

confidence: 82%

Improved preimplantation development of bovine ICSI embryos generated with spermatozoa pretreated with membrane-destabilizing agents lysolecithin and Triton X-100

et al. 2016

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“…Sperm plasma membrane integrity was assessed by flow cytometry after staining with SYBR®14 and propidium iodide (PI) (Goodla et al., ). Briefly, buffer B was added to aliquots of unselected and SLC‐selected samples to achieve a concentration of ~2 × 10 6 sperm cells/ml.…”

Section: Methodsmentioning

confidence: 99%

Effects of single layer centrifugation (SLC) on bull spermatozoa prior to freezing on post‐thaw semen characteristics

Nongbua

Johannisson

Edman³

et al. 2017

Reprod Domestic Animals

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Single layer centrifugation (SLC) has been shown to select the most robust spermatozoa from the ejaculate in several species. Here the effects of SLC prior to freezing on various parameters of frozen-thawed bovine sperm quality are reported. Semen from 8 bulls was layered on top of a species-specific colloid, Bovicoll. After centrifugation for 20 min at 300 g, the resulting sperm pellet was resuspended in OPTIXcell (IMV Technologies, l'Aigle, France); the SLC-selected sperm samples and uncentrifuged controls were frozen. On thawing, all sperm samples were analysed for membrane integrity, production of reactive oxygen species, mitochondrial membrane potential (MMP) and chromatin integrity. The SLC-treated samples had a higher percentage of live, superoxide-positive spermatozoa than uncentrifuged samples (27.9 ± 5.1% versus 21.7 ± 6.7%; p = .03). They had a higher proportion of spermatozoa with high mitochondrial membrane potential than uncentrifuged samples (55.9 ± 8.2% versus 40.5 ± 15.1%; p = .03) and also a lower proportion of spermatozoa with low mitochondrial membrane potential than non-treated samples (42.0 ± 8.5% versus 55.9 ± 14.4%; p = .04). No significant effects of treatment were found for membrane integrity or chromatin integrity. The effect of bull was significant on the proportions of dead, superoxide-positive spermatozoa and live, hydrogen peroxide-negative spermatozoa, as well as on membrane integrity, but it was not significant for mitochondrial membrane potential or chromatin integrity. These results suggest that SLC selects the most metabolically active bull spermatozoa from the rest of the population in normal ejaculates; the pattern of reactive oxygen species production may be different in SLC-selected spermatozoa compared to unselected samples.

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“…Previous studies with colloid centrifugation, using species -specific formulations of Androcoll, have reported beneficial effects in improving the motility, viability or mitochondrial activity of sperm samples, and removing dead cells, in red deer [10], boar [11], bull [12], or stallion [13], or buck [14]. In contrast, one of the main problems of the selection process with colloid centrifugation is the low yield in thawed samples.…”

Section: Introductionmentioning

confidence: 99%

Improving sperm banking efficiency in endangered species through the use of a sperm selection method in brown bear (Ursus arctos) thawed sperm

et al. 2017

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BackgroundSperm selection methods such as Single Layer Centrifugation (SLC) have been demonstrated to be a useful tool to improve the quality of sperm samples and therefore to increase the efficiency of other artificial reproductive techniques in several species. This procedure could help to improve the quality of genetic resource banks, which is essential for endangered species. In contrast, these sperm selection methods are optimized and focused on farm animals, where the recovery task is not as important as in endangered species because of their higher sperm availability. The aim of this study was to evaluate two centrifugation methods (300 x g/20 min and 600 x g/10 min) and three concentrations of SLC media (Androcoll-Bear −80, 65 and 50%) to optimise the procedure in order to recover as many sperm with the highest quality as possible. Sperm morphology could be important in the hydrodynamic relationship between the cell and centrifugation medium and thus the effect of sperm head morphometry on sperm yield and its hydrodynamic relationship were studied.ResultsThe samples selected with Androcoll-Bear 65% showed a very good yield (53.1 ± 2.9) although the yield from Androcoll-Bear 80% was lower (19.3 ± 3.3). The latter showed higher values of motility than the control immediately after post-thawing selection. However, both concentrations of colloid (65 and 80%) showed higher values of viable sperm and viable sperm with intact acrosome than the control. After an incubation of 2 h at 37 °C, the samples from Androcoll-Bear 80% had higher kinematics and proportion of viable sperm with intact acrosome. In the morphometric analysis, the sperm selected by the Androcoll-Bear 80% showed a head with a bigger area which was more elongated than the sperm from other treatments.ConclusionsWe conclude that sperm selection with Androcoll-Bear at either 65% or 80% is a suitable technique that allows a sperm population with better quality than the initial sample to be obtained. We recommend the use of Androcoll-Bear 65% since the yield is better than Androcoll-Bear 80%. Our findings pave the way for further research on application of sperm selection techniques to sperm banking in the brown bear.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1124-2) contains supplementary material, which is available to authorized users.

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Quality of bull spermatozoa after preparation by single-layer centrifugation

Cited by 45 publications

References 32 publications

Improved preimplantation development of bovine ICSI embryos generated with spermatozoa pretreated with membrane-destabilizing agents lysolecithin and Triton X-100

Improved preimplantation development of bovine ICSI embryos generated with spermatozoa pretreated with membrane-destabilizing agents lysolecithin and Triton X-100

Effects of single layer centrifugation (SLC) on bull spermatozoa prior to freezing on post‐thaw semen characteristics

Improving sperm banking efficiency in endangered species through the use of a sperm selection method in brown bear (Ursus arctos) thawed sperm

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