Quality of Bronchial Biopsies for Morphology Study and Cell Sampling: A Comparison of Asthmatic and Healthy Subjects

Labonté, Isabelle; Laviolette, Michel; Olivenstein, Ron; Chakir, Jamila; Boulet, Louis‐Philippe; Hamid, Qutayba

doi:10.1155/2008/202615

Cited by 12 publications

(23 citation statements)

References 18 publications

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“…For mucin 5A and extracellular matrix proteins, submucosal areas were measured with a calibrated image analysis system (Image Pro-Plus software, Media Cybernetics, Bethesda, Md) as previously reported. 18 The mean intraobserver variability (3 repeated cell counts) was between 4% and 8% for all studied antibodies. Mucin 5A was quantified by using Image Pro-plus software.…”

Section: Subjects' Evaluation Testsmentioning

confidence: 86%

“…18 Our institution's tissue bank processed the biopsies by using the same standardized methodology: tissues were fixed in 4% paraformaldehyde and embedded in paraffin. Sections (5 mm) were cut by using a microtome (Surgipath Medical Industries, Richmond, Ill).…”

Section: Subjects' Evaluation Testsmentioning

confidence: 99%

“…Qualitative evaluation (score) of bronchial tissues was done for the following criteria: general morphology, presence of smooth muscle bundles, inflammation, cartilage, mucus glands, basement membrane, and epithelial integrity. 18 Biopsies of poor quality were not kept. For morphological evaluation, sections were cut and stained with hematoxylin and eosin.…”

Section: Subjects' Evaluation Testsmentioning

confidence: 99%

“…Mucin 5A was stained by using mouse antihuman mucin 5A (Biodesign). [18][19][20] Sections were also stained with mouse antihuman type-I collagen, mouse antihuman type-III collagen (Calbiochem, Gibbstown, NJ), and mouse antihuman tenascin-c (Leica Microsystems, Bannockburn, Ill). Positive cells were counted in a blinded fashion at 3400 magnification.…”

Section: Subjects' Evaluation Testsmentioning

confidence: 99%

See 3 more Smart Citations

Airway remodeling and inflammation in competitive swimmers training in indoor chlorinated swimming pools

Bougault

Loubaki

Joubert

et al. 2012

Journal of Allergy and Clinical Immunology

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Section: Subjects' Evaluation Testsmentioning

confidence: 86%

Section: Subjects' Evaluation Testsmentioning

confidence: 99%

Section: Subjects' Evaluation Testsmentioning

confidence: 99%

Section: Subjects' Evaluation Testsmentioning

confidence: 99%

See 2 more Smart Citations

Airway remodeling and inflammation in competitive swimmers training in indoor chlorinated swimming pools

Bougault

Loubaki

Joubert

et al. 2012

Journal of Allergy and Clinical Immunology

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“…Our biopsies are probably taken deeper, leading to a greater amount of muscle collected in the control subjects than reported in other studies (alligator jaw forceps [FB-15C-1; Olympus, Centre Valley, PA] of volume 2.89 mm 3 were used). Our biopsies have been analyzed in a separate study for the SM area normalized to the total surface of the biopsy (62). No statistical differences were observed between subjects with mild to moderate asthma and control subjects.…”

Section: Total Smmhc In Asthmamentioning

confidence: 99%

Myosin, Transgelin, and Myosin Light Chain Kinase

Léguillette¹,

Laviolette²,

Bergeron³

et al. 2009

Am J Respir Crit Care Med

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Rationale: Airway smooth muscle (SM) of patients with asthma exhibits a greater velocity of shortening (Vmax) than that of normal subjects, and this is thought to contribute to airway hyperresponsiveness. A greater Vmax can result from increased myosin activation. This has been reported in sensitized human airway SM and in models of asthma. A faster Vmax can also result from the expression of specific contractile proteins that promote faster cross-bridge cycling. This possibility has never been addressed in asthma. Objectives: We tested the hypothesis that the expression of genes coding for SM contractile proteins is altered in asthmatic airways and contributes to their increased Vmax. Methods: We quantified the expression of several genes that code for SM contractile proteins in mild allergic asthmatic and control human airway endobronchial biopsies. The function of these contractile proteins was tested using the in vitro motility assay. Measurements and Main Results:We observed an increased expression of the fast myosin heavy chain isoform, transgelin, and myosin light chain kinase in patients with asthma. Immunohistochemistry demonstrated the expression of these genes at the protein level. To address the functional significance of this overexpression, we purified tracheal myosin from the hyperresponsive Fisher rats, which also overexpress the fast myosin heavy chain isoform as compared with the normoresponsive Lewis rats, and found a faster rate of actin filament propulsion. Conversely, transgelin did not alter the rate of actin filament propulsion. Conclusions: Selective overexpression of airway smooth muscle genes in asthmatic airways leads to increased Vmax, thus contributing to the airway hyperresponsiveness observed in asthma.

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The equine asthma model of airway remodeling: from a veterinary to a human perspective

Bullone

Lavoie

2019

Cell Tissue Res

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Human asthma is a complex and heterogeneous disorder characterized by chronic inflammation, bronchospasm and airway remodeling. The latter is a major determinant of the structure-function relationship of the respiratory system and is likely contributing to the progressive and accelerated decline in lung function observed in patients over time. Anti-inflammatory drugs such as corticosteroids are the cornerstone of asthma treatment. While their action on inflammation and lung function is well characterized, their effect on remodeling remains largely unknown. An important hindrance to airway remodeling as a major focus in asthma research is that the physiologic and clinical consequences of airway wall thickening and altered composition are not well understood. In this perspective, equine asthma provides a unique and ethical (non-terminal) preclinical model for hypothesis testing and generation. Severe equine asthma is a spontaneous disease affecting adult horses characterized by recurrent and reversible episodes of disease exacerbations. It is associated with bronchoalveolar neutrophilic inflammation, bronchospasm, excessive mucus secretion. Severe equine asthma is also characterized by bronchial remodeling, which is only partially improved by prolonged period of disease remission induced by therapy or antigen avoidance strategies. This review will focus on the similarities and differences of airway remodeling in equine and human asthma, on the strengths and limitations of the equine model, and on the challenges the model has to face to keep up with human asthma research.

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Quality of Bronchial Biopsies for Morphology Study and Cell Sampling: A Comparison of Asthmatic and Healthy Subjects

Abstract: A pool of six bronchial biopsy specimens can provide tissue of excellent quality in both asthmatic and healthy subjects and, consequently, a valuable sample for morphological analysis of mucosal structures.

Cited by 12 publications

References 18 publications

Airway remodeling and inflammation in competitive swimmers training in indoor chlorinated swimming pools

Airway remodeling and inflammation in competitive swimmers training in indoor chlorinated swimming pools

Myosin, Transgelin, and Myosin Light Chain Kinase

The equine asthma model of airway remodeling: from a veterinary to a human perspective

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