Quality of Alfalfa Herbage Estimated by a Prepared Cellulase Solution and Near Infrared Reflectance Spectroscopy

Bughrara, Suleiman S.; Sleper, D. A.; Belyea, Ronald L.; Marten, G. C.

doi:10.4141/cjps89-099

Cited by 9 publications

(3 citation statements)

References 14 publications

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“…The R 2 between 0.2 to 0.8 g L•• prepared cellulase solution treatments and NIRS spectra was 0.95 or greater. The SEC were acceptable for these prepared cellulase solution treatments and were similar to those reported before (Bughrara et al, 1989). The 0.8 g L-1 cellulase treatment was the most preferable treatment to estimate tall fescue IVDMD.…”

Section: Resultssupporting

confidence: 80%

“…The activity of crude cellulase has been generally measured by the degree of filter paper decomposition (Mandels and Weber, 1969). Bughrara et al (1989) indicated that the ideal assay to evaluate activity of a prepared cellulase solution should be simple and related to conditions under which the enzymes are to be used. They evaluated apparent activity of a prepared cellulase solution by digesting forage standards of known IVDMD.…”

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confidence: 99%

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Cellulase Solution Concentrations Used to Develop Spectral Equations for Predicting Tall Fescue Digestibility

1992

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Digestibility estimates obtained with prepared cellulase solutions and near infrared spectrophotometry (NIRS) have become valuable and reliable tools for forage quality determination. The objective of this study was to determine the optimum concentration of a prepared cellulase solution from a commercial source to obtain meaningful estimates of in vitro dry matter digestibility (IVDMD) and development of NIRS equations for tall fescue (Festuca arundinacea Schreb.). A total of 176 tall fescue samples were pretreated using pepsin and 0.125 M L−1 HCI followed by digestion using five different concentrations of a prepared cellulase solution obtained from Worthington Corp., Freehold, NJ. Concentrations of the prepared cellulase solution were 0.0, 0.2, 0.4, 0.6, and 0.8 g L−1. Maximum digestion occurred with the prepared cellulase solution at 0.57 g L−1. However, the most meaningful equation for estimating tall fescue IVDMD by NIRS occurred when samples were digested using the prepared cellulase solution at a concentration of 0.8 g L−1.

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Section: Resultssupporting

confidence: 80%

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Cellulase Solution Concentrations Used to Develop Spectral Equations for Predicting Tall Fescue Digestibility

1992

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“…This treatment was similar to the procedure outlined in Number 7 except the cellulase we used was produced by Boehringer Mannheim Biochemicals Co. (Indianapolis, IN). Apparent digestibility (IVDMD) was determined by a two-stage rumen fermentation procedure as described by Bughrara et al (1989). Fifteen milliliters of citrate buffer solution was added to each 50-milliliter glass tube contain- were injected into each tube with a hypodermic syringe.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Cellulase Solutions for Use in Digesting Forage Samples

1992

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Prepared cellulase solutions from different origins may differ in their relative effectiveness in providing accurate estimates of in vitro dry matter digestibility (IVDMD) of forages. Our objectives were to compare prepared cellulase solutions from different commercial sources with our non‐commercial prepared cellulase solution and evaluate their effectiveness in obtaining meaningful estimates of IVDMD for three grass and three legume species. A total of 46 samples were evaluated from six different forage species including switchgrass (Panicum virgatum L.), big bluestem (Andropogon gerardii Vitman), tall fescue (Festuca arundinacea Schreb.), alfalfa (Medicago sativa L.), birdsfoot trefoil (Lotus corniculatus L.), and lespedeza (Kummerowia striata Thunb.). Significant differences for IVDMD were found among the prepared cellulase solution sources. Correlations between IVDMD estimates using the prepared cellulase solutions and in vitro rumen fermentation ranged from 0.57 to 0.95. The highest correlation (r = 0.95) was observed using a non‐commercial prepared cellulase solution and the next (r = 0.91) was obtained from cellulase procured from Worthington Biochemical Corp (Freehold, NJ). We concluded that the cellulase solution prepared from cellulase obtained from Worthington Biochemical Corp. was an alternative to our non‐commercial source in obtaining estimates of IVDMD.

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Application of near‐infrared reflectance spectroscopy to chemical analysis of heterogeneous and botanically complex grassland samples

et al. 1993

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Abstract:The potential of near-infrared reflectance spectroscopy (NIRS) for determining the chemical composition of heterogeneous, botanically complex semi-natural grassland herbage was assessed. Samples were collected over four consecutive years at different maturation stages and were analysed by chemical and NIRS procedures for crude protein, neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin and cellulose. A subset of samples was selected on the basis of spectral features in order to establish calibration equations, while the other samples were used for validation. The wavelengths selected by using multiple regression were similar to others previously reported. The ranges of correlation coefficients and standard errors of prediction, respectively, for the various components when NIRS and chemical procedures were

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Quality of Alfalfa Herbage Estimated by a Prepared Cellulase Solution and Near Infrared Reflectance Spectroscopy

Cited by 9 publications

References 14 publications

Cellulase Solution Concentrations Used to Develop Spectral Equations for Predicting Tall Fescue Digestibility

Cellulase Solution Concentrations Used to Develop Spectral Equations for Predicting Tall Fescue Digestibility

Comparison of Cellulase Solutions for Use in Digesting Forage Samples

Application of near‐infrared reflectance spectroscopy to chemical analysis of heterogeneous and botanically complex grassland samples

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