Quality Evaluation of Traditional Chinese Medicine Compounds in Xiaoyan Lidan Tablets: Fingerprint and Quantitative Analysis Using UPLC-MS

Abstract

XiaoyanLidan tablets (XYLDTs) are traditional Chinese medicines frequently used for syndromes of the liver and gallbladder, cholecystitis and cholangitis. To evaluate the consistency of the quality of commercial XYLDT preparations, we established a simple and reliable ultra-performance liquid chromatography (UPLC) method with a photodiode array (PDA) detector and mass spectrometry (MS), including a fingerprint analysis and quantification of the main pharmacologically-active markers. In the UPLC-PDA detection-based fingerprint analysis of XYLDTs, approximately 39 peaks were found in the XYLDT chromatogram, 26 of which were attributed to Picrasmaquassioides, nine to Andrographis and four to Isodonserra. Subsequently, the structures of these bioactive markers were identified through ESI-MS analyses. Using the chemometricmethods of similarity analysis and principal component analysis, the five significant herbal componentswere determined as 4-methoxy-5-hydroxycanthin-6-one, andrographolide, dehydroandrographolide, neoandrographolide and rosmarinic acid, and these components were qualitatively assessed. Our experimental results demonstrated that combining the fingerprint analysis with UPLC-MS and multi-ingredient determination is useful for rapid pharmaceutical quality evaluation. Moreover, the combined approach can potentially differentiate the origin, determine the authenticity and assess the overall quality of the formulae.

Antibiofilm Activity of Extract and a Compound Isolated from Triumfetta welwitschii against Pseudomonas aeruginosa

“…Ultra-performance liquid chromatography (UPLC) is an improvement of the LC technique. UPLC employs chromatographic principles to separate compounds by utilising columns packed with smaller particles 1.7 μm in size and/or columns with higher flow rates [17]. is technique leads to a shorter analysis time, higher peak efficiency, and higher resolution.…”

Quality evaluation of Shenmaidihuang Pills based on the chromatographic fingerprints and simultaneous determination of seven bioactive constituents

“…PCA was also performed on the PPA to ensure that all elements equally influenced the results . Through the eigenvalues and percentage variance determined for each PC (Table ), we found that the first five PCs explained 94.2% of the relevant information.…”

Pharmacological effects of Picrasma quassioides (D. Don) Benn for inflammation, cancer and neuroprotection (Review)

“…Modern clinical research indicated that P. quassioides has a significant effect on hypertension (32), pneumonia (67,88), dysentery (89) and other disease symptoms (90,91). Previous studies have also developed various preparations and drugs using P. quassioides, such as Kumu injection, Xiaoyan Lidan tablet (92), Fufang Kumu Xiaoyan capsule (93) and Fufang Kumu Xiaoyan tablet (94). Thus, use of P. quassioides in clinical settings has been gaining considerable attention.…”