Quality Evaluation of Tetrastigmae Radix from Two Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis

Abstract

Tetrastigmae Radix, also known as Sanyeqing (SYQ) in Chinese, is an important traditional Chinese medicine with a long history. Tetrastigma hemsleyanum Diels et Gilg mainly grows in the south of the Yangtze River and is widely distributed. The content of bioactive constituents in SYQ varies greatly in different habitats, and there are obvious differences in the content of bioactive constituents between southwestern SYQ (WS) and southeastern SYQ (ES). To distinguish and evaluate the quality of ES and WS, an analytical method based on ultrafast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) was established for the simultaneous determination of 60 constituents including 25 flavonoids, 9 phenolic acids, 15 amino acids, and 11 nucleosides in 47 samples from ES and WS. In addition, orthogonal partial least squares discriminant analysis (OPLS-DA), t-test, and gray correlation analysis (GRA) were used to discriminate and evaluate the ES and WS samples based on the contents of 60 constituents. The results showed that there were significant differences in the bioactive constituents between ES and WS, and ES was superior to WS in terms of quality evaluation. This study not only provides basic information for differentiating ES and WS but also provides a new perspective for the comprehensive evaluation and quality control of SYQ from two different habitats.

UPLC-Q-TOF-MSE Based Profiling of the Chemical Composition of Different Parts of Tetrastigma hemsleyanum

“…The results indicated that the highest content of flavonoids, total phenols, and polysaccharides was found in the fibrous roots, followed by the leaves, and was the lowest in the tubers. Currently, the content of flavonoids, 28 phenolic acids, 29 and polysaccharides 28 is commonly used as a quality evaluation index of T. hemsleyanum, 30 providing a theoretical basis for subsequent selection and breeding. In this article, the names of different types of compounds and the cracking process are mainly clarified, but the quality markers of T. hemsleyanum are not reflected in this article.…”

Constituent Analysis and Quality Evaluation of Epimedii Folium from Different Species

“…Te validity of the approach was confrmed through assessments of its linearity, intraday and interday precision, repeatability, stability, recovery, and matrix efect [32]. Te standard curve was established by serial dilution of mixed standards, and the equation for linear regression, coefcient of correlation, and range for linearity was computed.…”

“…Te majority of studies have concentrated on the quantitative and qualitative analysis of favonoids with signifcant pharmacological efects and high content in a single species. However, it is widely recognized that the efcacy of TCMs is the outcome of the integrated adjustment of multiple components [31,32], emphasizing the need for ensuring the quality of TCMs to achieve their remarkable clinical efects. Yin et al [34] established a method based on UFLC-QTRAP-MS/MS technology for simultaneous determination of multiple active components in Abelmoschi Corolla, which provided an objective method reference for comprehensive evaluation and comprehensive control of Abelmoschi Corolla internal quality.…”

Screening the Extract of Laportea bulbifera (Sieb. et Zucc.) Wedd. Based on Active Component Content, Its Antioxidant Capacity and Exploration of Hepatoprotective Activity in Rats

“…It is worth noting that only one study reported the isolation of flavonoids from the ethyl acetate layer of LBR [11]. This discrepancy may be attributed to the significant variation in the content of bioactive constituents across different habitats and the potential influence of harvesting times on the accumulation of these constituents [16].…”