Quality Evaluation of Pulsatilla chinensis Total Saponin Extracts via Quantitative Analysis of Multicomponents by Single Marker Method Combined with Systematic Quantified Fingerprint Method

Abstract

Chinese medicine extracts are complex in composition. The combination of the quantitative analysis of multicomponents by single marker (QAMS) and the systematic quantified fingerprint method (SQFM) can be used for better quantitative analysis. The contents of Pulsatilla saponin D, Pulsatilla saponin A, Pulsatilla saponin F, and oleanolic acid 3-o-β-d-pyranoglucosyl-(1⟶4)-β-d-pyranoglucosyl-(1⟶3)-α-l-pyridine rhamnosyl-(1⟶2)-α-l-pyranosine arabinoside (B9) were determined by HPLC and QAMS. The methodological verification was carried out. The relative correction factor (RCF) was calculated, and the reproducibility of the RCF was investigated. The experimental results of the external standard method (ESM) and the QAMS were compared. Meanwhile, the fingerprint of the extract of Pulsatilla chinensis total saponins was established and the quality of the extract was evaluated by SQFM and hierarchical cluster analysis (HCA). The results showed that there was no significant difference between the QAMS and ESM. QAMS could be used for the rapid determination of various saponins in the extract of Pulsatilla chinensis. SQFM and HCA could objectively and comprehensively reflect the overall quality difference of total saponin extract of Pulsatilla chinensis. Therefore, QAMS and SQFM could provide a more convenient and effective selection for the quality evaluation of total saponin extract from this plant.

Identification of ROS and KEAP1‐related genes and verified targets of α‐hederin induce cell death for CRC

“…α-hederin, a pentacyclic triterpenoid saponin derived from Pulsatilla chinensis (Bunge) Regel (Ranunculaceae), has demonstrated effectiveness in various applications such as antitumor, anti-fungal, and insecticidal properties (Adamska et al, 2019;Chen et al, 2022). In this study, we found that α-hederin triggered oxidative stress events, including ROS accumulation and GSH depletion.…”

“…In the meantime, studies have tried to find effective phytochemicals or derivatives targeting the Nrf2/KEAP1 axis, which plays a crucial role in tumor development (Deshmukh et al, 2017; Fourquet et al, 2010; Yamamoto et al, 2008). α‐hederin, a pentacyclic triterpenoid saponin derived from Pulsatilla chinensis (Bunge) Regel (Ranunculaceae), has demonstrated effectiveness in various applications such as antitumor, anti‐fungal, and insecticidal properties (Adamska et al, 2019; Chen et al, 2022). In this study, we found that α‐hederin triggered oxidative stress events, including ROS accumulation and GSH depletion.…”

Quantitative analysis of multi-components by single marker method combined with UPLC-PAD fingerprint analysis based on saikosaponin for discrimination of Bupleuri Radix according to geographical origin

“…The combination of QAMS and chromatographic fingerprints technique enable simultaneous determinations of multiple target constituents and common peaks, which could largely improve the efficiency and comprehensiveness of the quality control of TCMs. Nowadays, the QAMS-fingerprint method has been successfully established and applied in many TCMs, such as Psoraleae Fructus (Luan et al, 2018), Radix Astragali (Huang et al, 2018), Rhubarb (Dou et al, 2021), Berberidis Cortex (Su et al, 2022), and Pulsatilla Chinensis (Chen et al, 2022), but there has been no relevant report in Bupleuri Radix. In addition, the current fingerprint method for Bupleuri Radix has a detection time of up to about 1.5 h, which is unable to applicate (Zhou et al, 2022).…”

Method for the Separation of Soybean Saponins from Soybean Meal by a Macroporous Adsorbent Resin: Optimization and Property Characterization

“…5 Saponins can be divided into steroid saponins and triterpene saponins according to their glycosides. 6 Steroidal saponins are found in monocotyledons such as panaxo saponins 7 and Silan saponins, 8 while triterpene saponins are found in dicotyledons such as bailey saponins 9 and soy saponins. 10 Soy saponins, in particular, have good amphiphilic properties with their polar water-soluble sugars linking to nonpolar nonsugar substances.…”