Quality evaluation of Platycladus orientalis (L.) Franco through simultaneous determination of four bioactive flavonoids by high-performance liquid chromatography

Lü, Yanhua; Liu, Zhiyong; Wang, Zhengtao; Wei, Dongzhi

doi:10.1016/j.jpba.2006.02.054

Cited by 45 publications

(25 citation statements)

References 13 publications

(13 reference statements)

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“…similar results were reported in the development of the analytical method for quercetin determination in the Platycladus orientalis (L.). At cited work obtaining LOD values of 0.005 μg/ml and LOQ values equivalent to 0.01 μg/ml [66]. The values difference in relation to the proposed method can be associated with several factors such as extractive matrix complexity, the method of determination of LOD parameters and LOQ up to detector sensitivity.…”

Section: Determination Of Sensitivitymentioning

confidence: 90%

Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

Souza

Andrade

Souza

et al. 2017

Int J Pharm Pharm Sci

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Objective: The Anadenanthera colubrina (Vell.) Brennan var. cebil is a medicinal plant that has been used for the treatment of many diseases in the northeastern region of Brazil. This plant contains secondary metabolites such as quercetin, a flavonoid that is known by its antioxidant and antiinflammatory effects. The aim of this work is to propose the validation of an analytical method using high-performance liquid chromatography with diode array detector (HPLC-DAD) for the quantification of quercetin and standardization of the hydroalcoholic extract (HAE) of A. colubrina. Methods:The A. colubrina extracts were prepared by the maceration process with powdered leaves at 20% weight: volume (w/v) and a hydroalcoholic solution at 50% volume: volume (v/v) for 120 h at room temperature. After pretreatment of the hydroalcoholic extract, the quercetin marker was used for quantification and proceeded to the evaluation of validation parameters for the method using HPLC-DAD. Results:The analytical method proved to be specific. Linear over the range 1.4-26.6 µg/ml, regression analysis showed a good correlation coefficient (R2= 0.999); the limit of detection (LOD) and the limit of quantification (LOQ) were 0.27 and 0.81 μg/ml respectively. The relative standard deviation (RSD) did not exceed 2.5% for precision. The proposed method was validated with an average recovery of 92.5-97.5%. Conclusion:The method was validated using HPLC-DAD, allowing the quantification of quercetin in the standardisation process of extracts and quality control of the herbal drug containing A. colubrina Phyto complex.

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Section: Determination Of Sensitivitymentioning

confidence: 90%

Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

Souza

Andrade

Souza

et al. 2017

Int J Pharm Pharm Sci

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“…CE, EAE and ME also showed considerable inhibitory activities against all the gram-positive bacteria. The abundant phenolic compounds might contribute the antibacterial activities of these extracts (Lu et al, 2006). Phenolic compounds are often involved in the degradation of cell walls and cell membranes, as well as the disruption of membrane functions such as electron transport, nutrient uptake, protein, nucleic acid synthesis and enzyme activity (Nychas, 1995).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Effects of Extracts of Thuja Orientalis cv Aurea Nana Cones against Food-spoilage and Food-borne Pathogens

Yang¹,

Hwang²,

Kwon³

et al. 2011

Korean Journal of Environmental Agriculture

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“…1). Herein we report the isolation and structural elucidation of isolates (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) propane ring in the molecule [11]. The 13 C NMR spectrum (l " Table 1) in combination with DEPT showed four tertiary methyls, three methines, four methylenes including a characteristic signal for the cyclopropane ring at δ C 10.9 [11], four quaternary carbons, and one methoxy carbon signals.…”

mentioning

confidence: 94%

“…which has been used for treatment of gout, rheumatism, diarrhea, and chronic tracheitis [1,2]. This tree has also been applied as a Korean traditional medicine remedy for hypertension, hematemesis, epistaxis, and hemorrhoids since ancient times [3].…”

mentioning

confidence: 99%

“…In the same context, the presence of the hydroxyl group at C-2 might be a positive influence on the activity against the SK-MEL-2 cell line considering the biological results of compounds 3 and 8 with the same skeletal structure. This plant (T. orientalis) has been recognized as an herbal medicine for treatment of various inflammatory diseases such as dermatitis, gout, and chronic tracheitis [1,2]. Moreover, the anti-inflammatory effects of T. orientalis have been reported through some researches [19,20].…”

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confidence: 99%

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Bioactive Sesquiterpenes from the Essential Oil of Thuja orientalis

et al. 2013

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!A phytochemical investigation on the essential oil of Thuja orientalis resulted in the isolation and identification of three new sesquiterpenes, 3α-methoxy-4α-epoxythujopsane (1), Δ 3,15 -4β-epoxythujopsene (2), and Δ 3,4 -thujopsen-2,15-diol (3), together with eight known sesquiterpenoids (4-11). The structures of these new compounds were elucidated based on spectroscopic data analyses including extensive 2D-NMR data and HR-ESIMS. The full assignments of 1 H and 13 C NMR chemical shifts for thujopsadiene (4) were obtained by 2D-NMR for the first time. All compounds (1-11) showed antiproliferative activities against the SK-OV-3 and SK-MEL-2 cell lines with IC 50 values of 5. compounds 1,3,4,7,8, and 9 significantly inhibited nitric oxide production in lipopolysaccharide-activated BV-2 cells with IC 50 values of 3.93-17.85 µM without cell toxicity.

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Quality evaluation of Platycladus orientalis (L.) Franco through simultaneous determination of four bioactive flavonoids by high-performance liquid chromatography

Cited by 45 publications

References 13 publications

Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

Antibacterial Effects of Extracts of Thuja Orientalis cv Aurea Nana Cones against Food-spoilage and Food-borne Pathogens

Bioactive Sesquiterpenes from the Essential Oil of Thuja orientalis

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