Quality Evaluation of Decoction Pieces of Gardeniae Fructus Based on Qualitative Analysis of the HPLC Fingerprint and Triple-Q-TOF-MS/MS Combined with Quantitative Analysis of 12 Representative Components

Xu, Jing; Zhou, Rongrong; Luo, Lin; Ying, Dai; Feng, Yaru; Dou, Zhihua

doi:10.1155/2022/2219932

Cited by 8 publications

(5 citation statements)

References 36 publications

(32 reference statements)

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“…The similarity of the fingerprints of the 24 batches of NLSCs ranged from 0.645 to 0.993. However, the similarity analysis had a few limitations—the huge crest covers the little top, which was fundamentally steady with the conclusion of the literature [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 84%

“…The stability of the same test solution was measured at 0, 2, 4, 8, 12, and 24 h. The RSDs values for the stability tests were less than 3.0% ( Table S4 ). The accuracy of the method was evaluated by the recovery, which was performed by adding the known mixed standard solutions into a certain amount of NLSC sample supplements [ 19 ]. The recoveries of the twenty-four investigated compounds extended from 80.11% to 104.3% ( Table S4 ), indicating the combination of HPLC-DAD and LC-MS/MS was exact and precise with palatable recuperations.…”

Section: Resultsmentioning

confidence: 99%

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Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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“…Both techniques have been used to separate, identify and quantify series of structurally different SMs in plants include spectrophotometric [57,[73][74][75], GC-MS [56,76], LC-MS [77], LC-MS/MS [56,76,78], Liquid Chromatography-High Resolution Electrospray Ionization Mass Spectrometry (LC-HRESIMS) [57], Nuclear Magnetic Resonance (NMR) [44,79], Ultra-Fast Liquid Chromatography (UFLC-Q-TOF-MS/MS) [80][81], UFLC-triple-Q-TOF-MS/MS [82], Ultra-high performance Liquid Chromatography quadrupole-time of flight mass spectrometry [83], UHPLC-LTQ-Orbitrap [84], UHPLC/Q-Orbitrap-MS [85], HPLC-DAD [86], UHPLC-Q-TOF-MS [87], UHPLC-Q-TOF-MS/MS [88], HPLC-DAD-ESI-MS [89], UPLC-QTOF/MSE [90], UHPLC-MS/MS [91], HPLC-ESI-QTOF-MS [92], UPLC-QTOF-MS/MS [93], UHPLC-Q-TOF-MS/MS [94][95], UHPLC-ESI-MS/MS [96], UHPLC-ESI-Orbitrap-MS/MS [97], UPLC-IT-MS [98], UPLC-Q-TOF-MS [98], UPLC-PDA [45], HPLC-ESI-MS and MS 2 [99], reverse phase high performance liquid chromatography-diode array detector (RP-HPLC-DAD) [74], HPLC-HRMS [100]. been used to produce a yellow color that turn colorless on addition of dilute hydrochloric acid confirm flavonoid [102,[104][105]…”

Section: Techniques For Identify and Quantifying Secondary Metabolitesmentioning

confidence: 99%

Identification and Pharmacological Activities of Secondary Metabolites of Phytochemicals

Ovonramwen¹

2022

Preprint

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Plant secondary metabolites are organic compounds (alkaloids, flavonoids, phenolics, tannins, anthraquinones, saponins, steroids, lignins, terpenes) produced by plant for self-defense during growth and development in order to protect them from harmful agents. As population is increasing and quality of life is reducing couple with outbreak of some diseases that are posing threat on the economy, a lot of medicinal plants have attracted interest of researchers because of the usefulness of these metabolites in pharmaceuticals, food and cosmetic industries. In this review, the pharmacological activities of some medicinal plants that are rich in secondary metabolites were studied, means of identifying and quantifying using spectrophotometry and chromatography techniques were also discussed. However, this will assist to reduce the uses and dependent on the synthetic drug and the onset of the age related diseases.

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“…High-performance liquid chromatography (HPLC) dominates the field of drug analysis with its high analysis speed and its wide range of applications, 20,21 mainly through the separation and identification of the chemical components of Chinese medicinal materials, providing assessment tools for their quality. [22][23][24] HPLC is widely used for similarity and difference analysis, determination of components, and identification and pharmacological analysis of Chinese herbal medicines. [25][26][27][28] Infrared (IR) spectroscopy has been widely applied in the identification of Chinese herbal materials owing to its characteristics of being non-destructive and fast, and it can be used to obtain the main structural information of the sample's chemical composition.…”

Section: Introductionmentioning

confidence: 99%

“…Chemical fingerprinting combined with chemometrics methods is currently one of the most commonly used methods for evaluating the chemical characteristics and identifying the origin of Chinese medicinal materials. High‐performance liquid chromatography (HPLC) dominates the field of drug analysis with its high analysis speed and its wide range of applications, 20,21 mainly through the separation and identification of the chemical components of Chinese medicinal materials, providing assessment tools for their quality 22–24 . HPLC is widely used for similarity and difference analysis, determination of components, and identification and pharmacological analysis of Chinese herbal medicines 25–28 .…”

Section: Introductionmentioning

confidence: 99%

Anticoagulant activity analysis and origin identification of Panax notoginseng using HPLC and ATR‐FTIR spectroscopy

Cui

Liu

et al. 2022

Phytochemical Analysis

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Introduction Panax notoginseng is one of the traditional precious and bulk‐traded medicinal materials in China. Its anticoagulant activity is related to its saponin composition. However, the correlation between saponins and anticoagulant activities in P. notoginseng from different origins and identification of the origins have been rarely reported. Objectives We aimed to analyze the correlation of components and activities of P. notoginseng from different origins and develop a rapid P. notoginseng origin identification method. Materials and methods Pharmacological experiments, HPLC, and ATR‐FTIR spectroscopy (variable selection) combined with chemometrics methods of P. notoginseng main roots from four different origins (359 individuals) in Yunnan Province were conducted. Results The pharmacological experiments and HPLC showed that the saponin content of P. notoginseng main roots was not significantly different. It was the highest in main roots from Wenshan Prefecture (9.86%). The coagulation time was prolonged to observe the strongest effect (4.99 s), and the anticoagulant activity was positively correlated with the contents of the three saponins. The content of ginsenoside Rg1 had the greatest influence on the anticoagulant effect. The results of spectroscopy combined with chemometrics show that the variable selection method could extract a small number of variables containing valid information and improve the performance of the model. The variable importance in projection has the best ability to identify the origins of P. notoginseng; the accuracy of the training set and the test set was 0.975 and 0.984, respectively. Conclusion This method is a powerful analytical tool for the activity analysis and identification of Chinese medicinal materials from different origins.

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Quality Evaluation of Decoction Pieces of Gardeniae Fructus Based on Qualitative Analysis of the HPLC Fingerprint and Triple-Q-TOF-MS/MS Combined with Quantitative Analysis of 12 Representative Components

Cited by 8 publications

References 36 publications

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Identification and Pharmacological Activities of Secondary Metabolites of Phytochemicals

Anticoagulant activity analysis and origin identification of Panax notoginseng using HPLC and ATR‐FTIR spectroscopy

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