Quality Control of the Root and Rhizome of Helminthostachys zeylanica (Daodi-Ugon) by HPLC Using Quercetin and Ugonins as Markers

Abstract

Daodi-Ugon is the dried root and rhizome of Helminthostachys zeylanica (L.) Hook. and has been used for centuries in the treatment of inflammation, fever, pneumonia, burns, and various disorders. However, the chromatographic methods to determine the phytochemical composition of H. zeylanica have never been reported. This study not only aims to develop a valid high-performance liquid chromatography (HPLC) method and to establish a chromatographic fingerprint for the quality control of H. zeylanica, it also establish the proposed content limits of Quercetin, Ugonin J, and Ugonin M. An HPLC method with a RP18 column (250 × 4.6 mm, 5 μm) was developed for the quantitative analysis of Quercetin, Ugonin J, and Ugonin M in H. zeylanica. A simple gradient of (A) methanol/(B) phosphoric acid in water (5–45 min, 70–80% A; 50–55 min, 80–70% A) was used and 360 nm was selected as the detection wavelength. The average contents and proposed content limits for H. zeylanica were calculated with a t-test and a measurement uncertainty test based on 20 batches of authentic H. zeylanica samples. Limits of detection (LOD), quantification (LOQ), linearity, precision, repeatability, stability, and recovery of the developed method were validated. All of the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of Quercetin, Ugonin J, and Ugonin M and to acquire the fingerprints of all of the collected H. zeylanica samples. At the 99% confidence level, the calculated content limits were 56.45, 112.15, and 277.98 mg/kg for Quercetin, Ugonin J, and Ugonin M, respectively. Those validated HPLC quantitative method, fingerprinting profile, and the proposed content limits of three chemical markers that could be used in the quality control of H. zeylanica in the market.

Hepatoprotective Effect of Ugonin M, A Helminthostachys zeylanica Constituent, on Acetaminophen-Induced Acute Liver Injury in Mice

“…Phytochemical research has reported that HZ is abundant in flavonoids [ 18 , 19 , 20 , 22 , 23 ]. Ugonin M, one of the major flavonoid components of HZ [ 34 ], exhibited both in vitro [ 19 , 23 ] and in vivo [ 24 ] antioxidant and anti-inflammatory activities. Thus, this study aimed to elucidate the role of Ugonin M in the pharmacological actions of HZ and subsequently to further discover the mechanism of actions of hepatoprotection viz APAP-induced acute liver injury in animal models [ 36 ].…”

“…Huang YC et al showed an in vitro anti-inflammatory activity of Ugonin M [ 19 ]. Our previous study showed through HPLC analysis that Ugonin M is one of the major representative components of HZ [ 34 ] and subsequently it was found to also present some protection effect from acute lung injury induced by lipopolysaccharides in the in vivo study [ 24 ]. The results of the previous effort aligned with the study from Suja SR et al whereby it showed that the crude extract of HZ possesses hepatoprotective effects against harmful substance [ 14 , 15 ].…”

Oligosaccharide-based quality evaluation of Atractylodis rhizome and a strategy for simplifying its quality control

“…Herbal medicine has gained popularity in many countries throughout the years. With the increased trend of usage worldwide, the assessment of safety, quality, and efficacy of these medicines has been an important concern for the public [1]. Atractylodis rhizome is a traditional perennial herb, which has been known and used as medication for a very long time.…”

Tablet Formula Optimization From Helminthostachys Zaylanica Extract Using A Simplex Lattice Design

“…Helminthostachys zeylanica known as Tunjuk Langit (TL), has active components, such as ugonstilben A-D and avonoids ugonin E-M (Chen et al, 2003;Fitrya, 2010;Wu et al, 2017) Pharmacological activities of the extract of TL has been reported as an antioxidant (Huang et al, 2003) , anti in ammatory (Huang et al, 2017;Su et al, 2016), hepatoprotective (Suja et al, 2004) , antihyperuricemia (Fitrya and Muharni, 2014) and antidiabetic (Chang et al, 2019;El Ridhasya et al, 2020). Based on a toxicity test of the extract, it was reported to be safe for consumption (Fitrya, 2017) .…”