Quality Control of Serum and Plasma by Quantification of (4E,14Z)-Sphingadienine-C18-1-Phosphate Uncovers Common Preanalytical Errors During Handling of Whole Blood

Liu, Xinyu; Hoene, Miriam; Yin, Peiyuan; Fritsche, Louise; Plomgaard, Peter; Hansen, Jørn Bindslev; Nakas, Christos T.; Nieß, Andreas M.; Hudemann, Jens; Haap, Michael; Mendy, Maimuna; Weigert, Cora; Wang, Xiaolin; Fritsche, Andreas; Peter, Andreas; Häring, Hans-Ulrich; Xu, Guowang; Lehmann, Rainer

doi:10.1373/clinchem.2017.277905

Cited by 31 publications

(39 citation statements)

References 30 publications

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“…The presence of SPD in the plasma, lymph, brain, and lungs of humans and/or mice has been reported . However, the process of characterizing SPD has been slower than that of other sphingoid bases.…”

Section: Introductionmentioning

confidence: 99%

“…20 The presence of SPD in the plasma, lymph, brain, and lungs of humans and/or mice has been reported. [21][22][23][24] However, the process of characterizing SPD has been slower than that of other sphingoid bases. Here we reveal the tissue distribution of SPD, the activity of sphingoid base-or sphingoid-base 1-phosphate-metabolizing enzymes toward SPD/ SPD 1-phosphate (SPD1P), the enzyme involved in SPD biosynthesis, the synthetic pathway of SPD, and the lipid microdomain localization of SPD-containing sphingolipids.…”

mentioning

confidence: 99%

“…SPD has a trans double bond between C4 and C5 and a cis double bond between C14 and C15. Although the existence of SPD in plasma, lymph, brain, and lung had previously been reported, [21][22][23][24] its exact tissue distribution remained unclear [Color figure can be viewed at wileyonlinelibrary.com] 2 | MATERIALS AND METHODS…”

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confidence: 99%

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Biosynthesis of the anti‐lipid‐microdomain sphingoid base 4,14‐sphingadiene by the ceramide desaturase FADS3

et al. 2020

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Sphingolipids are multifunctional lipids. Among the sphingolipid-component sphingoid bases, 4,14-sphingadiene (SPD) is unique such that it has a cis double bond with a bent structure. Although SPD was discovered half a century ago, its tissue distribution, biosynthesis, and degradation remain poorly understood. Here, we established a specific and quantitative method for SPD measurement and found that SPD exists in a wide range of mammalian tissues. SPD was especially abundant in kidney, where the amount of SPD was ~2/3 of sphingosine, the most abundant sphingoid base in mammals. Although SPD is metabolized to ceramides and SPD 1-phosphate with almost the same efficiency as sphingosine, it is less susceptible to degradation by a cleavage reaction, at least in vitro. We identified the fatty acid desaturase family protein FADS3 as a ceramide desaturase that produces SPD ceramides by desaturating ceramides containing sphingosine. SPD sphingolipids were preferentially localized outside lipid microdomains, suggesting that SPD has different functions compared to other sphingoid bases in the formation of lipid microdomains. In summary, we revealed the biosynthesis and degradation pathways of SPD and its characteristic membrane localization. Our findings contribute to the elucidation of the molecular mechanism underlying the generation of sphingolipid diversity. K E Y W O R D S4,14-sphingadiene, ceramide, fatty acid desaturase, lipid, sphingolipid How to cite this article: Jojima K, Edagawa M, Sawai M, Ohno Y, Kihara A. Biosynthesis of the anti-lipid-microdomain sphingoid base 4,14-sphingadiene by the ceramide desaturase FADS3. The FASEB Journal. 2020;34:3318-3335.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Biosynthesis of the anti‐lipid‐microdomain sphingoid base 4,14‐sphingadiene by the ceramide desaturase FADS3

et al. 2020

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“…Potential markers for sample quality have been studied using proteomics and metabolomics approaches (Anton et al 2015) (Kong et al 2017). Recently, Liu et al identified (4E,14Z)-sphingadienine-C18-1-phosphate as a biomarker that reflects deviations from SOPs during sample processing and can be used for assessing the quality of serum and plasma samples (Liu et al 2018). To the best of our knowledge no biomarkers for assessing the quality of tissue samples have so far been reported.…”

Section: Quality Control Measuresmentioning

confidence: 99%

Paramount importance of sample quality in pre-clinical and clinical research—Need for standard operating procedures (SOPs)

Rižner

Adamski

2019

The Journal of Steroid Biochemistry and Molecular Biology

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“…The correlative research in serum has so far not been widely reported. Nevertheless, accurate quantification of S1P levels in serum still poses many difficulties to modern LC‐MS/MS technology, mainly due to variable effects of a wide variety of biological matrices, as well as the lack of proper matrices free of analytes or samples with known concentrations of analytes …”

Section: Introductionmentioning

confidence: 99%

Validated LC‐MS/MS method of Sphingosine 1‐phosphate quantification in human serum for evaluation of response to radiotherapy in lung cancer

Tang

Chen

et al. 2020

Thoracic Cancer

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Background Sphingosine 1‐phosphate (S1P), a bioactive lipid, has been shown to mediate cancer processes. Therefore, accurate qualitative and quantitative determination is essential. The current assay method is still cumbersome to be of practical use worldwide and the aim of this study was therefore to develop a fast, accurate, precise and efficient LC‐MS/MS method for targeted analyses of S1P in serum samples. Methods Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) is an established method used for monitoring and analyzing S1P levels in serum. We determined the level of serum S1P in 256 patients with lung cancer and 36 healthy donors, and used Spearman';s rank correlation analysis to evaluate the difference in serum S1P levels between radiotherapy and nonradiotherapy patients. Results Standard curves were linear over ranges of 25–600 ng/mL for S1P with correlation coefficient (r2) greater than 0.9996. The lower limit of quantifications (LLOQs) was 25 ng/mL. The intra‐ and interbatch precisions and accuracy was less than 10% for S1P. The recoveries of the method were found to be 80%–98%. Serum S1P levels in healthy donors were different from those in patients (P < 0.001). Of 256 lung cancer patients, 124 (48.4%) received radiotherapy and were identified to have concomitant low serum S1P levels (222.13 ± 48.63), whereas 132 (51.6%) who had not received radiotherapy were identified to have high levels (315.16 ± 51.06). The serum S1P levels were therefore associated with radiotherapy (Spearman's Rho = −0.653, P < 0.001). Conclusions Our results indicated that this new LC‐MS/MS method is rapid, sensitive, specific and reliable for the quantification of S1P levels in serum samples. The level of S1P in serum samples of patients with lung cancer who received radiotherapy was significantly lower than that in patients who did not receive radiotherapy. Key points An improved method was established to quantify S1P levels in human serum by LC‐MS/MS, which enabled the change in serum S1P levels in lung cancer patients to be monitored, in combination with radiotherapy, and their clinical significance to be analyzed.

show abstract

Quality Control of Serum and Plasma by Quantification of (4E,14Z)-Sphingadienine-C18-1-Phosphate Uncovers Common Preanalytical Errors During Handling of Whole Blood

Cited by 31 publications

References 30 publications

Biosynthesis of the anti‐lipid‐microdomain sphingoid base 4,14‐sphingadiene by the ceramide desaturase FADS3

Biosynthesis of the anti‐lipid‐microdomain sphingoid base 4,14‐sphingadiene by the ceramide desaturase FADS3

Paramount importance of sample quality in pre-clinical and clinical research—Need for standard operating procedures (SOPs)

Validated LC‐MS/MS method of Sphingosine 1‐phosphate quantification in human serum for evaluation of response to radiotherapy in lung cancer

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