Quality Control Assessment of Human Immunodeficiency Virus Type 2 (HIV-2) Viral Load Quantification Assays: Results from an International Collaboration on HIV-2 Infection in 2006

Damond, Florence; Bénard, Antoine; Ruelle, Jean; Alabi, Abraham; Kupfer, Bernd; Gómes, Perpétua; Rodés, Berta; Albert, Jan; Böni, J; Garson, Jeremy A.; Ferns, Bridget; Matheron, Sophie; Chêne, Geneviève; Brun‐Vézinet, Françoise

doi:10.1128/jcm.00126-08

Cited by 42 publications

(35 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additional clinical laboratory requirements include criteria for generating reportable results, whether repeated measurements are made on samples, data on the resolution of false-positive/false-negative data, and the similarity of results from multiple laboratories that use the same and different technologies. Thus far, only a couple of interlaboratory comparisons have been performed, and both of these studies emphasized the need for standardization of qPCR diagnostic assays (44,45 ). Another interlaboratory exercise is planned within the European Framework 7 project: SPIDIA (Standardisation and Improvement of Generic Pre-analytical Tools and Procedures for In-Vitro Diagnostics; http://www.…”

Section: Research Vs Diagnostic Applicationsmentioning

confidence: 99%

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Beneš²,

et al. 2009

View full text Add to dashboard Cite

BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement. SUMMARY: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results

show abstract

Section: Research Vs Diagnostic Applicationsmentioning

confidence: 99%

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Beneš²,

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Specific molecular methods are therefore necessary for diagnosis and patient monitoring. Current assays, mainly consisting of in-house methods or unvalidated derivatives of commercial kits, suffer from major limitations in terms of their sensitivity, accuracy, and coverage of HIV-2 genetic diversity (19,20).…”

Section: Discussionmentioning

confidence: 99%

“…In-house assays are therefore widely used (12)(13)(14)(15)(16)(17)(18). The ACHI E V 2E international collaboration on HIV-2 infection showed that plasma HIV-2 RNA values vary considerably between laboratories (19,20). The high genetic diversity of HIV-2, with 9 groups designated A to I, of which only groups A and B are epidemic, also represents an obstacle to accurate viral load quantification (21)(22)(23)(24)(25).…”

mentioning

confidence: 99%

See 1 more Smart Citation

New Sensitive One-Step Real-Time Duplex PCR Method for Group A and B HIV-2 RNA Load

et al. 2014

Self Cite

View full text Add to dashboard Cite

. Analytic performances were determined in three laboratories. Clinical performances were evaluated on 100 plasma samples from HIV-2-infected patients (groups A, B, and H) by comparison with the assay currently used for the ANRS HIV-2 cohort. The specificity was 100%. Sensitivity was 50 copies/ml (cp/ml) and was optimized to 10 cp/ml. The within-run coefficients of variation in the three laboratories varied from 0.54% to 1.61% at 4 log 10 copies/ml and from 7.24% to 14.32% at 2 log 10 cp/ml. The between-run coefficients of variation varied from 2.28% to 6.43%. Of the 39 clinical samples below 2 log 10 in the current assay, the new test improved the detection or quantification of 17 samples, including eight group B samples. For quantifiable samples, similar loads were obtained with the two assays for group A samples. The median difference between the two assays for group B samples was ؉0.18 but with greater heterogeneity than for group A. The HIV-2 group H sample had similar results with the two assays. This new assay is highly sensitive and accurately quantifies the most prevalent HIV-2 groups. This test will be useful for monitoring low viral loads in HIV-2-infected patients.

show abstract

“…According to the commercial kits that are currently available, products of amplification can either be detected at the end of the reaction or while they accumulate in a real time manner. The lack of a commercially available viral load assay for HIV-2 is a concern for the proper management of patients infected with HIV-2 strains [106]. The resistance testing is actually an HIV-1 genotyping assay where the protease and the reverse transcriptase conserved regions of the pol gene are amplified and sequenced, as described by Fokam et al [107].…”

Section: Pcr Allows the Management Of Hiv Infectionmentioning

confidence: 99%

Application of PCR Technologies to Humans, Animals, Plants and Pathogens from Central Africa

Odile¹,

Migot-Nabias²,

Born³

et al. 2012

Polymerase Chain Reaction

View full text Add to dashboard Cite

Quality Control Assessment of Human Immunodeficiency Virus Type 2 (HIV-2) Viral Load Quantification Assays: Results from an International Collaboration on HIV-2 Infection in 2006

Cited by 42 publications

References 13 publications

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

New Sensitive One-Step Real-Time Duplex PCR Method for Group A and B HIV-2 RNA Load

Application of PCR Technologies to Humans, Animals, Plants and Pathogens from Central Africa

Contact Info

Product

Resources

About