Quality control assessment for the serological diagnosis of tick borne encephalitis virus infections

Niedrig, Matthias; Avšič, Tatjana; Aberle, Stephan W.; Ferenczi, Emöke; Лабуда, М; Rozentāle, Baiba; Mantke, Oliver Donoso

doi:10.1016/j.jcv.2006.12.013

Cited by 33 publications

(16 citation statements)

References 8 publications

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“…5, 31 and 37) reported false‐positive results on samples reactive for heterologous arboviruses or seronegative samples. Overall, the observed specificity was better than in past EQA studies [10–12]. As dengue virus and CHIKV co‐circulate in some regions, causing similar clinical symptoms, differential diagnosis between these arboviral infections is very important.…”

Section: Overall Results Of the External Quality Assurance For The Smentioning

confidence: 75%

“…These results clearly demonstrate that either the assays have to be improved or that they were not performed correctly. As described in previous EQA studies [10–12], reliable assays for IgM detection are a prerequisite for correct diagnosis of acute or recent infections, and their development is therefore crucial. Thirteen laboratories used commercial tests (12 used Anti‐Chikungunya virus IIFT IgM/IgG, Euroimmun AG; one used Chikungunya IgM Rapid Test, CTK Biotech Inc.) and 19 laboratories used in‐house tests.…”

Section: Overall Results Of the External Quality Assurance For The Smentioning

confidence: 99%

See 1 more Smart Citation

International diagnostic accuracy study for the serological detection of chikungunya virus infection

Niedrig

Zeller

Schuffenecker

et al. 2009

Clinical Microbiology and Infection

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External quality assurance for serological detection of chikungunya virus infection was performed to assess the diagnostic quality of expert laboratories. Of 30 participants, only six correctly analysed all reference samples with their respective tests. Thirteen laboratories gave at least 85% correct results, and 11 laboratories 75% or less. IgM antibodies were detected less frequently than IgG antibodies (p <0.001). The study provides information on the quality of different serological tests and indicates that most of the participants need to improve the sensitivity of their assays, in particular to detect IgM antibodies more reliably and be able to detect acute infections adequately.

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Section: Overall Results Of the External Quality Assurance For The Smentioning

confidence: 75%

Section: Overall Results Of the External Quality Assurance For The Smentioning

confidence: 99%

International diagnostic accuracy study for the serological detection of chikungunya virus infection

Niedrig

Zeller

Schuffenecker

et al. 2009

Clinical Microbiology and Infection

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“…The quality of these assays was assessed by an external quality assurance program of the European Network for the Diagnostics of 'Imported' Viral Diseases [19] . The laboratory performed well both in anti-TBEV IgM and anti-TBEV IgG detection from human samples.…”

Section: Serological Examination Of Goat Sera To Determine Immune Statementioning

confidence: 99%

Experimental Infection of Goats with Tick-Borne Encephalitis Virus and the Possibilities to Prevent Virus Transmission by Raw Goat Milk

et al. 2011

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Objectives: The aim of this work was to study the tick-borne encephalitis virus (TBEV) infection of goats and the possibilities to prevent human milk-borne infections either by immunizing animals or the heat treatment of milk. Methods: An experiment was conducted with 20 milking goats. Ten goats (half of them immunized) were challenged with live TBEV and 10 were left uninfected. Clinical signs and body temperatures of the animals were recorded and milk samples were collected daily. The presence of viral RNA and infectious virions in milk were detected by RT-PCR and intracerebral inoculation of suckling mice, respectively. Milk samples containing infectious virions were subjected to various heat treatment conditions and retested afterwards to assess the effect on infectivity. Results: The infected goats did not show any clinical signs or fever compared to uninfected ones. Infectious virions were detected for 8–19 days from the milk samples (genome for 3–18 days by PCR) of infected goats. Immunized goats did not shed the virus. After heat treatment of the milk, the inoculated mice survived. Conclusions: Goats shed the virus with their milk without showing any symptoms. Human milk-borne infections can be avoided both by immunizing goats and boiling/pasteurizing infected milk.

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“…For current laboratory diagnosis of TBE, serological assays are usually used [31,32]. In particular, IgM capture ELISA has been widely used for rapid diagnosis [33], because IgM antibody is detected in serum or CSF in the early phase of disease.…”

Section: Discussionmentioning

confidence: 99%

Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification

Hayasaka

Aoki

Morita

2013

Virol J

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BackgroundTick-borne encephalitis virus (TBEV) is a causative agent of acute central nervous system disease in humans. It has three subtypes, far eastern (FE), Siberian (Sib) and European (Eu) subtypes, which are distributed over a wide area of Europe and Asia. The objective of this study was to develop a simple and rapid assay for the detection of TBEV RNA by using reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) method that can differentiate the three subtypes of TBEV and can be used for clinical diagnosis and epidemiological study.MethodsPrimers for TBEV-specific and subtype-specific RT-LAMP assay were designed to target the consensus sequence in NS1 of all subtypes and the consensus sequence in the E gene of each subtype, respectiveluy. In vitro transcribed RNA of Oshima strain that belongs to FE subtype was serially diluted and used to examine the sensitivity of the assay. Cross-reactivity of subtype-specific RT-LAMP assay was tested by using the RNA of Oshima and Sofjin (FE), IR-99 (Sib) and Hochosterwitz (Eu) strains. RNA extracted from the mixtures of TBEV and ticks, and of TBEV and human blood, and the mouse tissues infected with TBEV, were evaluated in the assay. Positive amplification was observed by real-time monitoring of turbidity and by visual detection of color change.ResultsThe sensitivity of TBEV-specific RT-LAMP assay was 102 copies of target RNA per reaction volume. FE-specific RT-LAMP assay amplified viral genes of Oshima and Sofjin strains but not of IR-99 and Hochosterwitz strains, and of Japanese encephalitis virus. RT-LAMP assay for Sib and for Eu specifically amplified viral genes of IR-99 and Hochosterwitz strains, respectively. We also showed that tick or human blood extract did not inhibit the amplification of viral gene during the assay. Furthermore, we confirmed that the TBEV RT-LAMP could detect virus RNA from peripheral and central nervous system tissues of laboratory mice infected with TBEV.ConclusionTBEV RT-LAMP assay offers a sensitive, specific, rapid and easy-to-handle method for the detection of TBEV RNA in tick samples and this may be applied in the clinical samples collected from TBE-suspected patients.

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Quality control assessment for the serological diagnosis of tick borne encephalitis virus infections

Cited by 33 publications

References 8 publications

International diagnostic accuracy study for the serological detection of chikungunya virus infection

International diagnostic accuracy study for the serological detection of chikungunya virus infection

Experimental Infection of Goats with Tick-Borne Encephalitis Virus and the Possibilities to Prevent Virus Transmission by Raw Goat Milk

Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification

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