Quality Control and Standardization of Vaccines

Thornton, Denise H.

doi:10.1007/978-1-4613-2613-7_10

Cited by 6 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Characterization of the challenge virus isolated from field samples was confirmed by PCR [ 14 , 28 ]. We used 10 4 PFU of the isolated virus for challenge as reported by Thornton [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…The virus was detected by PCR assay using a set of primers FP: GGATCG CCC AC CACG ATTAC TACC and RP: ACTG CCT CACACAACC TCATC TCC [ 14 ]. The utilized isolated MDV was titrated in duck embryo fibroblast (DEF) according to Thornton [ 15 ], and used for the experimental infection of chicks of different breeds. Eight different native breeds namely (Gimmizah, Sinai, Dandarawi, Fayoumi, Golden Montazah, Matrouh, Beheri, Dokki) in addition to SPF Lohmann, and High Line of 1 day old which carry ChB6 gene and group of chicken (Bovans) which lacks ChB6 gene of 1 day old and all have no maternal antibodies against MDV [ 16 ] were used for the experiment.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genetic resistance of eight native Egyptian chicken breeds having chicken B-cell marker 6 gene post-challenge with field strain of Marek's disease-induced tumor virus

et al. 2018

View full text Add to dashboard Cite

Aim:The aim of this work was to detect chicken B-cell marker 6 (ChB6) gene in some native breeds in Egypt and find the relationship between founded genes in these different breeds to determine the resistance of native Egyptian breeds of chicken to Marek’s disease (MD).Materials and Methods:A total of 14 different chicken breeds (30 each) including ten native breeds in addition to SPF Lohmann, High Line, Bovans, and Roodiland were used. Blood samples were collected for the detection of (ChB6) by polymerase chain reaction (PCR) assay and sequenced to determine the presence or absence of ChB6 gene. Experimental infection was done using local field isolated MD virus (MDV) of 11 (1 day old) unvaccinated chick breeds having no maternal antibodies against MDV. Ten breeds of them carry ChB6 gene, eight breeds were native, and the rest two breeds were SPF Lohmann and High Line in addition to a group of ChB6 gene-lacking breed (Bovans) were infected. Spleen samples were collected from all infected breeds at 20th, 25th, 30th, 35th, and 40th weeks post-infection and tested by PCR assay for the detection of MDV. Furthermore, at 40th week post-infection, tumorized spleen sample of Bovans breed was collected and prepared for examination by transmission electron microscope (TEM) to confirm the presence of MDV.Results:Our results revealed the positivity of 10 out of 14 breeds (Gimmizah, Sinai, Dandarawi, Fayoumi, Golden Montazah, Matrouh, Beheri, Dokki, SPF Lohmann, and High Line) to the presence of ChB6 gene and resistance to MDV infection, while the Bovans, Mandarah, Inshas and Roodiland breeds lack the ChB6 gene and are susceptible to MDV infection. The collected spleen samples revealed negative for the presence of challenged MDV by PCR in 10 breeds (Gimmizah, Sinai, Dandarawi, Fayoumi, Golden Montazah, Matrouh, Beheri, Dokki, SPF Lohmann, and High Line) and positive for Bovans breed. TEM is used to confirm MDV infection in Bovans group which demonstrated tumors.Conclusion:The study confirms the relationship between the presence of ChB6 gene in our native breeds and the absence of tumors.

show abstract

“…Characterization of the challenge virus isolated from field samples was confirmed by PCR [ 14 , 28 ]. We used 10 4 PFU of the isolated virus for challenge as reported by Thornton [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%