Quality control and determination of melatonin in food supplements

Cerezo, Ana B.; Leal, A Marques; Álvarez-Fernández, M. Antonia; Hornedo-Ortega, Ruth; Troncoso, Ana M.; García‐Parrilla, M. Carmen

doi:10.1016/j.jfca.2015.09.013

Cited by 45 publications

(27 citation statements)

References 39 publications

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“…Melatonin is a popular food supplement with human health benefits [ 59 ]. It was first reported to be present in apple fruit in 2013 [ 19 ]; therefore, it is expected that apple juice should be rich in melatonin.…”

Section: Discussionmentioning

confidence: 99%

Melatonin in Apples and Juice: Inhibition of Browning and Microorganism Growth in Apple Juice

Zhang

Chen

et al. 2018

Molecules

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Synthetic melatonin (N-acetyl-5-methoxytryptamine, MT) is popular in the US and Asian markets as a health supplement. Here, we identified a naturally occurring melatonin source in apple juice. Melatonin was present in all 18 apple cultivars tested. The highest melatonin level of the edible part of apple was detected in the apple peel. The melatonin content in ‘Fuji’ apple juice is comparable to the level of its flesh. Melatonin was consumed during the process of juicing due to its interaction with the oxidants. Melatonin addition significantly reduced the juice color change to brown (browning). The mechanism is that melatonin scavenges the free radicals, which was indicated by the ASBT analysis; therefore, inhibiting the conversion of o-diphenolic compounds into quinones. Most importantly, melatonin exhibited powerful anti-microorganism activity in juice. The exact mechanisms of this action are currently unknown. These effects of melatonin can preserve the quality and prolong the shelf life of apple juice. The results provide valuable information regarding commerciall apple juice processing and storage.

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Section: Discussionmentioning

confidence: 99%

Melatonin in Apples and Juice: Inhibition of Browning and Microorganism Growth in Apple Juice

Zhang

Chen

et al. 2018

Molecules

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“…In fact, it has been observed that a moderate intake of beer (330–660 mL) significantly increased melatonin concentration in plasma [20]. Moreover, melatonin supplements are widely commercialized in Europe [35]. The European Union considers food supplements as food, and thus they are regulated by European food law [36,37].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of VEGF-Induced VEGFR-2 Activation and HUVEC Migration by Melatonin and Other Bioactive Indolic Compounds

et al. 2017

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Excessive concentrations of vascular endothelial growth factor (VEGF) trigger angiogenesis, which causes complications such as the destabilization of atherosclerotic plaques and increased growth of tumors. This work focuses on the determination of the inhibitory activity of melatonin and other indolic related compounds on VEGF-induced VEGF receptor-2 (VEGFR-2) activation and an approximation to the molecular mechanism underlying the inhibition. Quantification of phosphorylated VEGFR-2 was measured by ELISA. Migration wound-healing assay was used to determine cell migration of human umbilical vein endothelial cells (HUVECs). This is the first time that melatonin, 3-indolacetic acid, 5-hydroxytryptophol, and serotonin are proved to significantly inhibit VEGF-induced VEGFR-2 activation in human umbilical vein endothelial cells and subsequent angiogenesis. 3-Indolacetic acid showed the highest inhibitory effect (IC50 value of 0.9704 mM), followed by 5-hydroxytryptophol (35% of inhibition at 0.1 mM), melatonin (30% of inhibition at 1 mM), and serotonin (24% of inhibition at 1 mM). An approximation to the molecular mechanism of the inhibition has been proposed, suggesting that indolic compounds might interact with the cell surface components of the endothelial membrane in a way that prevents VEGF from activating the receptor. Additionally, wound-healing assay revealed that exposure of HUVECs to melatonin and 3-indolacetic acid in the presence of VEGF significantly inhibited cell migration by 87% and 99%, respectively, after 24 h. These data demonstrate that melatonin, 3-indolacetic acid, 5-hydroxytryptophol, and serotonin would be good molecules for future exploitation as anti-VEGF signaling agents.

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“…These products have become very attractive because of their non-prescription status, quite low prices, and the opinion that natural products are safe. They are regulated by European food law (Regulation (EC) 178/2002 and Directive, 2002/46/EC) and considered as food [24]. It's been verified that ML supplements can be considered as efficient and safe in insomnia therapy and as an anti-oxidant [25,26].…”

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confidence: 99%

Sensitive Electrochemical Detection Method of Melatonin in Food Supplements

Miccoli¹,

Restani²,

Floroian³

et al. 2018

Rev. Chim.

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Melatonin (N-acetyl-5-methoxytryptamin) is a ubiquitous molecule widely distributed in nature. It is a hormone produced by the pineal gland, which plays a role in the body�s sleep cycles and it is a powerful antioxidant. It is possible to find it in several foods (fruit, rice, corn) and as food supplement. Screen-printed carbon electrode (DRP-150) and graphene modified screen-printed electrode (DRP-110GPH) were tested as sensors for the detection of melatonin using electrochemical detection (differential pulse voltammetry), at different pH values (7.4, 7.0, and 6.4). Quantitative detection of melatonin was possible, with better results when graphene modified screen-printed electrodes were used. The pH influenced the position of the peak as well, with lowering the pH moves more in the right. Both electrodes have been tested on samples of food supplements containing melatonin, with good recovery degrees.

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Quality control and determination of melatonin in food supplements

Cited by 45 publications

References 39 publications

Melatonin in Apples and Juice: Inhibition of Browning and Microorganism Growth in Apple Juice

Melatonin in Apples and Juice: Inhibition of Browning and Microorganism Growth in Apple Juice

Inhibition of VEGF-Induced VEGFR-2 Activation and HUVEC Migration by Melatonin and Other Bioactive Indolic Compounds

Sensitive Electrochemical Detection Method of Melatonin in Food Supplements

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