Quality-by-design model in optimization of PEG-PLGA nano micelles for targeted cancer therapy

Eskandari, Zahra; Kazdal, Fatma; Bahadorı, Fatemeh; Ebrahimi, Nabiallah

doi:10.1016/j.jddst.2018.10.009

Cited by 18 publications

(7 citation statements)

References 40 publications

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“…Default setting on the Zetasizer was used, including refractive index and absorption, the dispersant used was water and measurement angle was 173. Measurements were repeated 5 times, 3 min each and data were analysed by intensity distribution …”

Section: Methodsmentioning

confidence: 99%

“…Measurements were repeated 5 times, 3 min each and data were analysed by intensity distribution. [19] Determination of optimized nano-esculetin formulation Increasing amounts of esculetin (10-25 mg) were successively uploaded to 50 mg of PLGA (in 20 ml) using abovementioned method, and PS was measured. Excess amounts of esculetin outpouring from PLGA micelles caused disruption in the mono-dispersity of particles.…”

Section: Determination Of Particle Size (Ps)mentioning

confidence: 99%

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Investigation of cell death mechanism and activity of esculetin-loaded PLGA nanoparticles on insulinoma cellsin vitro

Kaçar

Bahadorı

Tekkeli

et al. 2020

Journal of Pharmacy and Pharmacology

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Aim The purpose of this study was to prepare targeted cancer therapy formulation against insulinoma INS‐1 cells and to study its effect on cell death with related mechanisms in vitro. Methods Polylactide‐co‐glycolide (PLGA) nano‐micelles were used for preparation of esculetin nano‐formulation (nano‐esculetin). The cells were treated with nano‐esculetin and free esculetin. Apoptotic and necrotic cell death percentages, cell proliferation, ATP and GTP reductions and insulin levels were investigated on insulinoma INS‐1 cells for both free and nano‐esculetin formulations. Results About 50 mg of PLGA was able to carry 20 mg esculetin in 20 ml of formulation. The obtained optimized formulation was 150 nm, with 92% encapsulation efficiency and a slow‐release behaviour was observed during release studies. Nano‐esculetin bearing 25, 50 and 100 μg esculetin and free esculetin in equivalent doses successfully decreased cell viability. The prevailing cell death mechanism was necrosis. Along with cell proliferation, intracellular insulin and the ratio of ATP and GTP were decreased even with 12.5, 25 and 50 μg esculetin bearing nano‐formulation and its equivalent free esculetin. Conclusions The results revealed that esculetin is able to show its anti‐tumor afficacy after loading to PLGA nano‐micelles and nano‐encapsulation intensifies its cytotoxic activity in vitro. Current study shows that esculetin and its nano formulations are promising agents in treatment of insulinoma.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of Particle Size (Ps)mentioning

confidence: 99%

Investigation of cell death mechanism and activity of esculetin-loaded PLGA nanoparticles on insulinoma cellsin vitro

Kaçar

Bahadorı

Tekkeli

et al. 2020

Journal of Pharmacy and Pharmacology

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“…The subsequent mathematical treatment enables the optimization of experimental conditions to get the desired result and also indicates which parameters lead to synergistic or antagonist effects. This approach has been regularly used for the formulation of lipidic vectors [153] and is also increasingly assessed for polymeric systems [154][155][156][157]. To our knowledge, only two examples have performed DOE for PDT vectors.…”

Section: Ps Positioningmentioning

confidence: 99%

Rational design of block copolymer self-assemblies in photodynamic therapy

Demazeau¹,

Gibot²,

Mingotaud³

et al. 2020

Beilstein J. Nanotechnol.

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Photodynamic therapy is a technique already used in ophthalmology or oncology. It is based on the local production of reactive oxygen species through an energy transfer from an excited photosensitizer to oxygen present in the biological tissue. This review first presents an update, mainly covering the last five years, regarding the block copolymers used as nanovectors for the delivery of the photosensitizer. In particular, we describe the chemical nature and structure of the block copolymers showing a very large range of existing systems, spanning from natural polymers such as proteins or polysaccharides to synthetic ones such as polyesters or polyacrylates. A second part focuses on important parameters for their design and the improvement of their efficiency. Finally, particular attention has been paid to the question of nanocarrier internalization and interaction with membranes (both biomimetic and cellular), and the importance of intracellular targeting has been addressed.

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“…Measurements were performed five times, three minutes each and the data was analyzed in terms of number, intensity and volume distribution. Although, the "average size" presented by Zetasizer was used in QbD calculations (Eskandari et al, 2018). Laser Doppler micro-electrophoresis method using a folded capillary zeta cell (Malvern Instruments Ltd) was used to measure ZP.…”

Section: Determination Of Ps Poly Dispersity Index (Pdi) and Zpmentioning

confidence: 99%

Development and optimization of a novel PLGA-Levan based drug delivery system for curcumin, using a quality-by-design approach

Bahadorı

Eskandari

Ebrahimi

et al. 2019

European Journal of Pharmaceutical Sciences

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Quality-by-design model in optimization of PEG-PLGA nano micelles for targeted cancer therapy

Cited by 18 publications

References 40 publications

Investigation of cell death mechanism and activity of esculetin-loaded PLGA nanoparticles on insulinoma cellsin vitro

Investigation of cell death mechanism and activity of esculetin-loaded PLGA nanoparticles on insulinoma cellsin vitro

Rational design of block copolymer self-assemblies in photodynamic therapy

Development and optimization of a novel PLGA-Levan based drug delivery system for curcumin, using a quality-by-design approach

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