Quality-Assured Biobanking: The Leiden University Medical Center Model

Haumann, Rianne; Verspaget, Hein W.

doi:10.1007/978-1-4939-7592-1_27

Cited by 6 publications

(7 citation statements)

References 12 publications

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“…A total of 500 μL serum is stored at −20°C immediately after processing and transferred to −80°C freezers within maximally 8 hours after collection. ( 23 ) Serum levels of cytokines were analyzed using electrochemiluminescent immunoassays from Meso Scale Discovery, Rockville, MD, USA; IL‐1β, IL‐6, IL‐8, TNF‐α, IL‐10, IFN‐γ, IL‐12p70, IL‐13, IL‐2, and IL‐4 with V‐Plex Proinflammatory panel 1 (K15049D‐1) and IL‐17A, IL‐21, IL‐22, IL‐23, IL‐27, IL‐31, and macrophage inflammatory protein‐3 alpha (MIP‐3α) with using V‐Plex Plus Th17 Panel 1 Human Kit (K15085G‐1). All analyses were conducted according to the manufacturer's recommendations and without additional freeze/thaw cycles and with the eight‐point calibration curve, providing a dynamic range, analyzed in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Immunological Characterization of Chronic Nonbacterial Osteomyelitis (CNO) in Adults: A Cross‐Sectional Exploratory Study

Leerling,

Andeweg,

Faber

et al. 2023

JBMR Plus

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Chronic nonbacterial osteomyelitis (CNO) is a rare disease spectrum affecting children and adults. Adult CNO may occur as isolated bone inflammation, or with a broad range of extraskeletal features. CNO pathophysiology, including the key drivers of inflammation, remains largely unknown. For pediatric CNO, a role for pro‐inflammatory cytokine dysregulation has been proposed, but studies in adults are scarce. We therefore provide immunological characterization of adult CNO. Cross‐sectional study in our referral center including adult CNO patients (n = 172) and healthy controls (n = 65). Inflammation parameters and systemic inflammatory based scores(SIBS, including neutrophil/lymphocyte ratio [NLR] and systemic immune inflammation index [SII]) were compared between groups. Cytokine expression was explored with electrochemiluminescent immunoassays in 33 patients, eight healthy controls and 21 osteoporosis patients. Routine inflammation markers were higher in patients than in controls, but generally remained within reference range. Systemic inflammation was more pronounced in patients with additional vertebral involvement as compared to those osteitis in the anterior chest wall alone, in patients with comorbid pustulosis palmoplantaris or psoriasis, and in patients with strongly rather than moderately increased lesional uptake on nuclear imaging. SII was elevated in CNO patients too, but NLR was not. Cytokine expression was generally nondifferential between patients and both control groups, and patients displayed low absolute concentrations of pro‐inflammatory cytokines. In this adult CNO cohort, systemic inflammation was generally subtle, but more pronounced in patients with vertebral lesions, associated skin disease, and strongly increased uptake on nuclear imaging. SII was increased in patients compared to healthy controls. Contrasting pediatric studies, we found no increased expression of the pro‐inflammatory cytokines that have been proposed to drive the inflammatory cascade, like interleukin‐6, ‐8, and ‐17 (IL‐6, IL‐8, and IL‐17), and tumor necrosis α (TNF‐α). Further studies are needed to evaluate the use of SII in diagnosis and monitoring of CNO, and elucidate the role of cytokine dysregulation in adult disease. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.

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Section: Methodsmentioning

confidence: 99%

Immunological Characterization of Chronic Nonbacterial Osteomyelitis (CNO) in Adults: A Cross‐Sectional Exploratory Study

Leerling,

Andeweg,

Faber

et al. 2023

JBMR Plus

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“…In this cohort study 96 serum samples were collected from a IRB approved biobank [20]. All samples were retrieved from patients ≥18 years of age who visited the outpatient clinic of the Center for Bone Quality, Department of Endocrinology of the Leiden University Medical Center in the Netherlands between 2015 and 2021 and retrieved a definitive diagnosis of any subtype of FD/MAS.…”

Section: Subjectsmentioning

confidence: 99%

“…All samples were retrieved from patients ≥18 years of age who visited the outpatient clinic of the Center for Bone Quality, Department of Endocrinology of the Leiden University Medical Center in the Netherlands between 2015 and 2021 and retrieved a definitive diagnosis of any subtype of FD/MAS. All samples were taken before the afternoon with a maximum processing time of 4 hours [20]. This study was approved by the Medical Ethics Committee and scientific biobank committee [20].…”

Section: Subjectsmentioning

confidence: 99%

Clinical Value of RANKL, OPG, IL-6 and Sclerostin as Biomarkers for Fibrous Dysplasia/McCune-Albright Syndrome

Meier

Hagelstein-Rotman

Streefland

et al. 2023

Preprint

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“…Standardized strategies and best practice protocols for blood collection and manipulation have been proposed to reduce pre-analytical variables [16][17][18]. International standard ISO 15189, which specifies requirements for quality and competence in medical laboratories, contains several sections focused on the pre-analytical phase [19].…”

Section: Introductionmentioning

confidence: 99%

The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes

et al. 2022

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Background Blood is a common source of RNA for gene expression studies. However, it is known to be vulnerable to pre-analytical variables. Although RNA stabilization systems have been shown to reduce such influence, traditional EDTA tubes are still widely used since they are less expensive and enable to study specific leukocyte populations. This study aimed to assess the influence of storage time and temperature between blood sampling and handling on RNA from peripheral blood mononuclear cells (PBMCs). Methods and results Nine blood samples were collected in EDTA tubes from 10 healthy donors. One tube from each donor was immediately processed for PBMC isolation, while the others were first incubated at either 4 degrees Celsius (°C) or room temperature for 2, 4, 6 and 24 h. RNA yield and quality and the expression level of fourt housekeeping (B2M, CASC3, GAPDH, HPRT1) and 8 target genes (CD14, CD19, CD20, IL10, MxA, TNF, TNFAIP3, NR4A2) were compared between samples. RNA yield, quality and integrity did not vary significantly with time and temperature. B2M was the most stable housekeeping gene, while the others were increasingly influenced by storing time, especially at 4 °C. Even when normalized to B2M, the expression level of some target genes, particularly TNFAIP3 and NR4A2, was highly affected by delays in blood processing at either temperature, already from 2 h. Conclusion Pre-analytical processing has a great impact on transcript expression from blood collected in EDTA tubes, especially on genes related to inflammation. Standardized procedure of blood handling are needed to obtain reliable results.

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Quality-Assured Biobanking: The Leiden University Medical Center Model

Cited by 6 publications

References 12 publications

Immunological Characterization of Chronic Nonbacterial Osteomyelitis (CNO) in Adults: A Cross‐Sectional Exploratory Study

Immunological Characterization of Chronic Nonbacterial Osteomyelitis (CNO) in Adults: A Cross‐Sectional Exploratory Study

Clinical Value of RANKL, OPG, IL-6 and Sclerostin as Biomarkers for Fibrous Dysplasia/McCune-Albright Syndrome

The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes

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