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iii AcknowledgmentsWe gratefully acknowledge the wastewater-treatment operators and managers for granting access to their plants, providing advice on sampling locations, and providing in-kind services during sampling. In addition to the cooperating agencies, Ohio Environmental Protection Agency (OEPA) and the U.S. Environmental Protection Agency (USEPA) provided in-kind services, and Kubota and Ovivo provided financial support for this study. We thank Stefan Wuertz (University of California, Davis) for help with quantifying viruses and Fu-Chih Hsu (Scientific Methods, Granger, Ind.) for providing reagents and advice on the EasyPhage method. We also thank the peer-review team for their help and advice throughout the study: Paul Concentrations of bacterial indicators are given in colony-forming units per 100 milliliters (CFU/100 mL).
Concentrations of viruses are given in genomic copies per liter (gc/L).Concentrations of coliphage are given in plaque-forming units per 100 milliliters (PFU/100 mL).Specific conductance is given in microsiemens per centimeter at 25 degrees Celsius (µS/cm at 25°C).
Quantifying Viruses and Bacteria in
AbstractMembrane bioreactors (MBR), used for wastewater treatment in Ohio and elsewhere in the United States, have pore sizes small enough to theoretically reduce concentrations of protozoa and bacteria, but not viruses. Sampling for viruses in wastewater is seldom done and not required. Instead, the bacterial indicators Escherichia coli (E. coli) and fecal coliforms are the required microbial measures of effluents for wastewater-discharge permits. Information is needed on the effectiveness of MBRs in removing human enteric viruses from wastewaters, particularly as compared to conventional wastewater treatment before and after disinfection.A total of 73 regular and 28 quality-control (QC) samples were collected at three MBR and two conventional wastewater plants in Ohio during 23 regular and 3 QC sampling trips in 2008-10. Samples were collected ...