Quality Assurance and Validation

Butler, John M.

doi:10.1016/b978-0-12-374513-2.00007-5

Cited by 5 publications

(16 citation statements)

References 187 publications

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“…Adli genetikte en yaygın olarak hızlı mutasyona uğrayan (10 -4 ila 10 -2 mpg) çoklu alelik varyasyonlar gösteren Y-STR belirteçleri kullanılmaktadır (Gettings, 2015;Butler ve Hill, 2012) Adli bilimlerde kullanılan bir diğer Y-kromozomu belirteci olan Y-SNP'ler ise yavaş mutasyona (gen başına ortalama 10 -8 ila 10 -9 mutasyon, mpg) uğrayan, insan soyunu tahmin etmenin yanı sıra evrimsel göç kalıplarını incelemede kullanılan tek baz varyasyonlu bi-alelik belirteçlerdir (Balanovsky, 2017;Butler, 2012;Nachman, 2000). Bugüne kadar, Uluslararası Genetik Geneoloji Derneği insan Y-kromozom Tarayıcısı veri tabanına göre 700.000'den fazla Y-SNP belirlenmiştir (International Society of Genetic Genealogy Human Y-chromosome Browser, ISOGG YBrowse) (ybrowse.…”

Section: Dizilemeunclassified

Olay Yerlerinden Biyolojik Delillerin Toplanması ve Saklanması

AKKAN,

ÖĞDÜR

2024

Adli Biyoloji: Doğanın İzleriyle Adaletin Peşinde II

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Teknoloji ilerledikçe ve uluslararası kalite standartları benimsendikçe, adli tıp laboratuvarları da delilleri daha yüksek düzeyde inceleme, saklama ve analiz etme uzmanlığıyla donatılmaktadır. Bu gelişmeler sayesinde adli bilim insanları adaletin ihtiyaçlarına daha iyi hizmet edebilir ve kolluk kuvvetlerine çok değerli yardımlar sağlayabilir. Suç mahallinden elde edilen bulguların kalitesinin, adli tıp laboratuvarları tarafından gerçekleştirilen analizlerin doğruluğu ve güvenilirliği ile doğrudan bağlantılı olduğunu unutmamak gerekir. Bu nedenle, doğru ve güvenilir sonuçlar elde etmek için toplanan bulguların en yüksek kalitede olmasını sağlamak kritik öneme sahiptir. Olay yerinden toplanan kanıtların davaya mümkün olduğunca katkı sağlaması için incelemenin büyük bir dikkatle yapılması gerekmektedir. Numunelerin tanınması, tespit edilen bulguların doğru bir şekilde toplanması ve uygun yöntemlerle laboratuvarlara gönderilmesi önemlidir. Bunu yaparak, delillerin doğru bir şekilde analiz edilmesini sağlayabilir ve suçtan etkilenenlere adaletin yerini bulmasına yardımcı olabilecek değerli bilgiler sunabiliriz. Bu bölümde olay yeri inceleme yöntemlerinin temelleri ele alınarak, olay yerinde yaygın bulunan biyolojik örnek türleri ve ülkemizde uygulanan delil toplama yöntemleri ve prosedürleri anlatılacaktır

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Section: Dizilemeunclassified

Olay Yerlerinden Biyolojik Delillerin Toplanması ve Saklanması

AKKAN,

ÖĞDÜR

2024

Adli Biyoloji: Doğanın İzleriyle Adaletin Peşinde II

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“…Reliable data comparison among laboratories is also enabled by standard data formats and core DNA testing regions. One of the primary reasons that DNA is on a more solid scientific foundation compared to many of the other forensic disciplines is a community-wide use of standard methods and materials to produce quality measurements [ 70 ].…”

Section: International Standards In Forensic Dnamentioning

confidence: 99%

“…Standard 9.5.5 in the revised Quality Assurance Standards states: “The laboratory shall check its DNA procedures annually or whenever substantial changes are made to the protocol(s) against an appropriate and available NIST standard reference material or standard traceable to a NIST standard” [ 82 ]. NIST supplies several DNA standard reference materials (SRMs) to enable validation of a laboratory’s measurement capabilities as well as calibration of instrumentation and methods (see Table 7.7 in [ 70 ]).…”

Section: Physical Reference Standardsmentioning

confidence: 99%

U.S. initiatives to strengthen forensic science & international standards in forensic DNA

Butler

2015

Forensic Science International: Genetics

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A number of initiatives are underway in the United States in response to the 2009 critique of forensic science by a National Academy of Sciences committee. This article provides a broad review of activities including efforts of the White House National Science and Technology Council Subcommittee on Forensic Science and a partnership between the Department of Justice (DOJ) and the National Institute of Standards and Technology (NIST) to create the National Commission on Forensic Science and the Organization of Scientific Area Committees. These initiatives are seeking to improve policies and practices of forensic science. Efforts to fund research activities and aid technology transition and training in forensic science are also covered. The second portion of the article reviews standards in place or in development around the world for forensic DNA. Documentary standards are used to help define written procedures to perform testing. Physical standards serve as reference materials for calibration and traceability purposes when testing is performed. Both documentary and physical standards enable reliable data comparison, and standard data formats and common markers or testing regions are crucial for effective data sharing. Core DNA markers provide a common framework and currency for constructing DNA databases with compatible data. Recent developments in expanding core DNA markers in Europe and the United States are discussed.

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“…3 Many parameters such as baseline noise, sensitivity, inhibitor tolerance, accuracy and precision are used in the internal validation. 4 Also, these parameters can be used to evaluate and compare commercially available kits. [5][6] GlobalFiler™ IQC PCR Amplification Kit (GF-IQC) consists of 21 autosomal loci, amelogenin and 2 Y-chromosome markers with an additional IQC (internal quality control system) marker, whereas PowerPlex ® Fusion 6C System (P-6C) consists of 23 autosomal loci, amelogenin and 4 Y-chromosome markers.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Efficiency of GlobalfilerTM IQC PCR Amplification Kit and Powerplex® Fusion 6C System with Half-Volume Reactions for Forensic Trace DNA Testing

Nattawoot Saokaew,

Kewalee Junpan,

Wawkan Duangshatome

et al. 2022

Indian Journal of Forensic Medicine & Toxicology

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Two major challenges in daily and routinely forensic genetics test are samples with low or very low quantities of DNA and high cost of processing. To overcome these obstacles, a reduction in reaction volume to half would offer a substantial benefit, but even so it should provide complete genetic profiles without conceding the quality of the results. The aim of this study is to test the robustness of two commercially available kits, GlobalFiler™ IQC PCR Amplification Kit (GF-IQC) and PowerPlex ® Fusion 6C System (P-6C), with half volume reaction in accordance with SGWDAM guidelines. GF-IQC showed minimum and stochastic threshold of 45 and 610 RFU, while P-6C demonstrated 60 and 670 RFU of those values respectively. The P-6C can tolerate to many inhibitors including melanin, hematin, and humic acid. Both kits showed the same performance on case work profiling and the sensitivity of allele detection of P-6C was not significantly different from that of . The results of this study demonstrated that GF-IQC and P-6C with half volume reaction can produce good quality profiles and could be applied for forensic DNA examination.

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Quality Assurance and Validation

Cited by 5 publications

References 187 publications

Olay Yerlerinden Biyolojik Delillerin Toplanması ve Saklanması

Olay Yerlerinden Biyolojik Delillerin Toplanması ve Saklanması

U.S. initiatives to strengthen forensic science & international standards in forensic DNA

Comparison of the Efficiency of GlobalfilerTM IQC PCR Amplification Kit and Powerplex® Fusion 6C System with Half-Volume Reactions for Forensic Trace DNA Testing

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