Quality assessment on Polygoni Multiflori Caulis using HPLC/UV/MS combined with principle component analysis

Abstract

BackgroundPolygoni Multiflori Caulis, the dried caulis of Polygonum multiflorum Thunb., is one of the commonly used traditional Chinese medicines having antioxidant, anti-obesity, anti-inflammatory and antibacterial effects. Polygoni Multiflori Caulis used clinically or circulated on market have great differences in their diameters. However, to the best of our knowledge, no study has been reported on the qualities of Polygoni Multiflori Caulis with different diameters.ResultsSystematic HPLC/UV/MS chromatographic fingerprinting and quantitative analytical methods combined with principal component analysis were developed and applied to analyze different Polygoni Multiflori Caulis samples. The contents of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, the chemical marker for quality control on Polygoni Multiflori Caulis specified in Chinese Pharmacopoeia (2010 edition), were found to have surprising relevance with the samples’ diameters for the first time.ConclusionThe finding provides a scientific basis for collecting Polygoni Multiflori Caulis in the best time. Moreover, the diameter can be used as the criterion for quality control on Polygoni Multiflori Caulis as a preliminary step in the future. In addition, scores plot obtained from principal component analysis shows the obvious differences between unqualified Polygoni Multiflori Caulis samples and qualified ones visually, which can be used to single out the unqualified ones with qualified ones efficiently and immediately.

Identification of the absorbed components and their metabolites of Tianma‐Gouteng granule in rat plasma and bile using ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

“…The prototype components were identified or tentatively identified by comparing their retention times and TOF–MS data with those of authentic standards (Figure S3 and S4) or with the literature (Chen et al, , Huang et al, , Tang, Wang, Liu, Cheng, & Xiao, , Yang et al, , Zhao, Kao, et al, ). The known compounds were identified by comparing the accurate mass and retention time with authentic standards, and the unknown compounds were tentatively characterized by comparing the accurate mass and tandem mass spectrometric data with previous research findings.…”

“…To obtain a clearer view of the absorbed prototype components and metabolites profiles of TGG, the base-peak intensity chromatograms of high-drug-concentration plasma and bile samples were extracted in both positive and negative ion modes rather than a total ion chromatogram (Supporting Information Figures S1 and S2). The extracted ion chromatograms (EIC) of TOF-MS for the absorbed prototype components and metabolites of TGG are presented in Figures 1 and 2, which could avoid endogenous interference from complex biological matrices that may affect identification of the absorbed components and metabolites (Zhou et al, 2017), rather than base-peak intensity and total ion chromato- Table 1. The prototype components were identified or tentatively identified by comparing their retention times and TOF-MS data with those of authentic standards ( Figure S3 and S4) or with the literature (Chen et al, 2014, Huang et al, 2016, Tang, Wang, Liu, Cheng, & Xiao, 2016, Zhao, Kao, et al, 2013. The known compounds were identified by comparing the accurate mass and retention time with authentic standards, and the unknown compounds were tentatively characterized by comparing the accurate mass and tandem mass spectrometric data with previous research findings.…”

Rapid Characterization of the major chemical constituents from Polygoni Multiflori Caulis by liquid chromatography tandem mass spectrometry and comparative analysis with Polygoni Multiflori Radix

“…Recently, a number of chromatographic methods have been established to determine the major constituents of PMC through HPLC and LC–MS , but there is no study about comprehensive analysis of the chemical composition. Additionally, different pharmacological effects always result from variant chemical ingredients.…”

Simultaneous determination of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β -D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography

“…[43] The loading plot showed in Figure 4. Marker b and d contributed to higher PC2 score and c contributed mainly to PC1 score.…”