“…Flow virometry experiments were performed with a CytoFLEX LX (Beckman Coulter Inc., Brea, CA, USA) with a Violet SSC (VSSC) 405 nm filter, as reported previously. 39 The instrument was standardized using Megamix-Plus SSC and FSC fluorescent polystyrene beads (0.1, 0.16, 0.20, 0.24, and 0.5 μm; Biocytex, Marseille, France) as a quality control tool. The threshold of the trigger signal (VSSC) was manually adjusted to 1500, and gains were set as 95, 9, and 115 for FSC, VSSC, and B525-FITC lasers, respectively.…”