Quality assessment of Cortex Phellodendri by high‐performance liquid chromatography coupled with electrospray ionization mass spectrometry

Abstract

A simple method based on liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) was developed for the quality assessment of Cortex Phellodendri (CP), which was mainly derived from two species of Phellodendron chinense Schneid and Phellodendron amurense Rupr. Total 41 compounds, including 14 phenols, 24 alkaloids and three liminoidal triterpenes were identified or tentatively characterized from the 75% methanol extract of CP samples by online ESI-MS(n) fragmentation and UV spectra analysis. Among them, two phenols and six alkaloids were simultaneously quantified using HPLC-DAD method. The validated HPLC-DAD method showed a good linearity, precision, repeatability and accuracy for the quantification of eight marker compounds. Furthermore, the plausible fragmentation pathway of the representative compounds were proposed in the present study. The differences of the chemical constituents content and the comprehensive HPLC profiles between the two CP species using LC-DAD-ESI-MS method are reported for the first time, indicating that the CP drugs from different resources should be used separately in the clinic.

Bu-Shen-Ning-Xin decoction suppresses osteoclastogenesis <i>via </i>increasing dehydroepiandrosterone to prevent postmenopausal osteoporosis

“…The extract of this herb suppresses the synthesis of collagen and promoted the synthesis of proteoglycans in chondrocytes (33). Alkaloids are the principal active components of the bark of the Chinese Corktree (33).…”

Metabolic fate analysis of Huang–Lian–Jie–Du Decoction in rat urine and feces by LC–IT-MS combining with LC–FT-ICR-MS: a feasible strategy for the metabolism study of Chinese medical formula

“…. Combined with their chromatography behavior analysis, lotusine and oblongine were excluded because of faster elution than magnolflorine (Hu et al, 2010). However, magnocurarine was eluted slower than magnolflorine by comparison with standard (Nikolić et al, 2012).…”

Plasma pharmacochemistry based approach to screening potential bioactive components in Huang-Lian-Jie-Du-Tang using high performance liquid chromatography coupled with mass spectrometric detection

“…Peaks 7 and 8 are the other types of alkaloids. As the reference chemicals unavailable, they were tentatively identified by retention behaviors, MS and MS/MS spectra with those of literature data (Liang et al, 2006;Hu et al, 2009). All the flavonoids detected in the experiment (peaks 9, 10, 16, 17, 18, 19, 20 and 22) (Han et al, 2007), it was tentatively identified as oroxylin A-7-O-glucuronide.…”