Quality and standardisation of Royal Jelly

Sabatini, A. G.

doi:10.3896/ibra.4.01.1.04

Cited by 196 publications

(211 citation statements)

References 28 publications

(17 reference statements)

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“…In the RJ samples the three major carbohydrate compounds (glucose, fructose and sucrose) were identified along with the minore carbohydrate compounds like turanose, maltose, trehalose and erlose. The three major carbohydrate compounds account for 7-8% in RJ according to Sabatini et al (2009). In the RJ samples studied the major carbohydrates had a mean value of 15.39±0.33%.…”

Section: Resultsmentioning

confidence: 99%

Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

Mărgăoan¹,

Mărghitaș²,

Dezmirean³

et al. 2017

BUASVMCN-ASB

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Given their beneficial effects in terms of health, the natural products, especially beehive products, have drawn the attention of consumers since long time ago. In order to guarantee the quality of these products on the market, their chemical composition needs to be analyzed. Thus, this current research had as objective the establishment of quality parameters for beehive brood food derived products: apilarnil and queen bee larvae triturate. These two products were compared with royal jelly which is the basis of brood food in the first 3 days of larval stage. The carbohydrates were determined by HPLC-IR and allowed the identification of seven carbohydrate compounds, predominantly glucose, fructose and sucrose. The lipid profile was analyzed by the Soxhlet method. The total protein content was determined by the Kjeldahl method. Free amino acids were analyzed by LC-MS. A total of 31 amino acids were identified of which nine are essential amino acids for humans.

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Section: Resultsmentioning

confidence: 99%

Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

Mărgăoan¹,

Mărghitaș²,

Dezmirean³

et al. 2017

BUASVMCN-ASB

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“…Souza et al, 2015) could be used in future studies to confirm that the in vitro workers were indeed workers and not intercastes. One of the difficulties in including royal jelly in a standardized larval rearing protocol is that royal jelly composition varies from one royal jelly source and batch to the next (Sabatini, Marcazzan, Caboni, Bogdanov, & Almeida-Muradian, 2009;Zheng, Hu, & Dietemann, 2010). We discovered that the source of royal jelly is critical in larval rearing success.…”

Section: Larval Diet Source and Compositionmentioning

confidence: 98%

Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

Schmehl

Tomé

Mortensen

et al. 2016

Journal of Apicultural Research

128

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The in vitro rearing of worker honey bees (Apis mellifera L.) has become an increasingly important method in honey bee research in general, and in pesticide risk assessment specifically. Authorities from the European Organization for Economic Co-operation and Development and the United States Environmental Protection Agency are requesting data on pesticide impacts on immature bee survivorship prior to registering new crop protection products. Those using the current in vitro rearing protocols have had variable success with immature bee survival and protocol repeatability. Here, we present an improved method for the in vitro rearing of worker honey bees from larvae to adult emergence. We have achieved consistently high survival (>95%) in our control and solvent-control rearing trials. Changes in the proportion of diet components, royal jelly source, maintenance of the developing bee, and rearing environment are the main contributors for our high rearing success and are discussed herein. Our in vitro rearing protocol can be implemented as the standard protocol to determine the impact of pesticides on immature bees because of the protocol's high control survivability, ease in end point determination, and high overall repeatability. Protocolo para la cría in vitro de obreras de Apis melliferaLa cría in vitro de las abejas obreras (Apis mellifera L.) se ha convertido en un método cada vez más importante en la investigació n de la abeja de la miel en general, y específicamente, en la evaluació n del riesgo de los plaguicidas. Las autoridades de la Organizació n Europea para la Cooperació n y el Desarrollo, y la Agencia de Protecció n Ambiental de los Estados Unidos están solicitando datos sobre los efectos de los plaguicidas en la supervivencia de abejas inmaduras antes de registrar nuevos productos para la protecció n de los cultivos. Aquellos que utilizan actualmente los protocolos de la cría in vitro han tenido un éxito variable en la supervivencia de abejas inmaduras y la repetitividad del protocolo. Aquí presentamos un método mejorado para la cría in vitro de las abejas obreras a partir de larvas para la eclosió n de los adultos. Hemos logrado consistentemente una alta supervivencia (>95%) en nuestro control y en los ensayos de cría con el disolvente del control. Los cambios en la proporció n de los componentes de la dieta, la fuente de jalea real, el mantenimiento de las abejas en desarrollo, y el ambiente de cría son los principales constituyentes para nuestro alto éxito de cría y son discutidos en este trabajo. Nuestro protocolo de cría in vitro puede ser implementado como el protocolo estándar para determinar el impacto de los plaguicidas en las abejas inmaduras debido a la alta capacidad de supervivencia del control, la facilidad en la determinació n del punto final, y la alta capacidad de repetitividad.

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“…However, when stored at room temperature, the lipid content did not change, but the proline and lysine content increased during the first three months, which suggests that if temperature is favorable, it continues to proteolytic activity over time (SABATINI et al, 2009). …”

Section: Physicochemical Quality Of Royal Jelly Samplesmentioning

confidence: 99%

“…Marconi et al (2002) investigated ways to define the freshness of royal jelly using furosine content; Sabatini et al (2009) used glucose oxidase, and more recently, Kamamura (2011) reported royalactina as responsible for queen differentiation in fresh royal jelly.…”

Section: Physicochemical Quality Of Royal Jelly Samplesmentioning

confidence: 99%