Received: August 30, 2013VLÁŠEK VÁCLAV, LANGOVÁ JITKA, ŠTENCL JIŘÍ: Eff ect of modifi ed atmosphere packaging on stability of three kinds of bread. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 6, pp. 1881-1887 The study evaluates eff ect of ordinary and modifi ed atmosphere N 2 and CO 2 on bread packaging and changes of selected bread parameters, moisture content (MC) and water activity (Aw), in real storage time. MC has a signifi cant infl uence on physical properties of bread and Aw characterizes its microbiological stability. Both parameters are functionally dependent. Manometric method was used for measurement of Aw and gravimetric method was used for measurement of MC. Samples of three kinds of bread, gluten-free, Šumava bread, and Bavorský bread, packaged into barrier wrappers, were stored at the room temperature of 20 °C. MC and Aw were measured in real storage time in intervals of three days for all kinds of bread, always in crust and crumb separately. Individual measurements were fi nished when a mould were discovered in samples.