Quality and reactive oxygen species of extended canine semen after vitamin C supplementation

Michael, A.J.; Alexopoulos, C.; Pontiki, Eleni; Hadjipavlou‐Litina, Dimitra; Saratsis, Ph.; Ververidis, H.N.; Boscos, C.

doi:10.1016/j.theriogenology.2008.05.043

Cited by 39 publications

(38 citation statements)

References 38 publications

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“…Moreover, supplementation of skim milk powder at 7.5 mg/mL contributed to positive effects on sperm viability. These results may indicate that skim milk may protect boar sperm from the generation of excess ROS, which is known to exert physical and chemical effects on sperm quality (Michael et al, 2008). On the other hand, our results revealed that supplementation with high concentrations of skim milk powder (≥ 30 mg/mL) had a negative effect on sperm quality parameters of boar semen preserved at 15 °C.…”

Section: Discussionmentioning

confidence: 53%

“…The ROS may accumulate during the storage of semen in the absence of appropriate antioxidants in the semen extender. Once an imbalance between the production and removal of ROS occurs, the generation of excessive ROS subjects the spermatozoa to oxidative stress (Michael et al, 2008). In general, antioxidants prevent oxidant-mediated damage by free radicals or their reactive metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…ever, when an imbalance between excessive production and accumulation of ROS and antioxidant defence mechanisms (oxidative stress) occurs, it leads to sperm damage and reduced semen quality, resulting in decreased sperm motility, capacity for sperm-oocyte fusion, and fertility (Michael et al, 2008). Enzymatic (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymatic (e.g.…”

mentioning

confidence: 99%

“…Enzymatic (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymatic (e.g. ascorbic acid, α-tocopherol, taurine and albumin) antioxidant systems are effective defence mechanisms of sperm and seminal plasma against the toxic effects of oxidative stress (Griveau et al, 1995;Michael et al, 2008;Bustamante Filho et al, 2009).…”

mentioning

confidence: 99%

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Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

Namula

Kodama

Tanihara

et al. 2014

Acta Veterinaria Hungarica

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This study investigated the effects of skim-milk supplementation on the quality and penetrating ability of boar semen preserved at 15 °C. When boar semen samples were preserved in Modified Modena extender supplemented with various concentrations (0, 7.5, 15, 30 and 50 mg/mL) of skim milk powder at 15 °C for 4 weeks, higher sperm motility and viability were observed in the case of 7.5 mg/mL skim-milk supplementation compared with the control group (0 mg/mL) during the preservation (P < 0.05). When in vitro matured oocytes were co-incubated with boar sperm that had been preserved in Modified Modena extender with three different concentrations (0, 7.5 or 15 mg/mL) of skim milk powder at 15 °C for two weeks, there were no apparent effects of skim-milk supplementation on the rates of fertilisation and development to blastocysts of oocytes after co-incubation. However, the monospermic fertilisation rate of sperm preserved with 15 mg/mL skim milk powder was higher (P < 0.05) than that of fresh non-preserved sperm, but did not differ among the preservation groups. The results indicate that the supplementation of Modified Modena extender with 7.5 mg/mL skim milk powder improves the motility and viability, but not the penetrating ability, of sperm after liquid preservation for at least two weeks.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

Namula

Kodama

Tanihara

et al. 2014

Acta Veterinaria Hungarica

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“…The morphological sperm abnormalities/defects were determined by examining semen smears stained with eosin-nigrosin on a glass slide (Vilakazi and Webb, 2004;Michael et al, 2008). Sperm abnormalities were classified as described by Zemjanis (1970) and Sekoni (1992).…”

Section: Preparation Of Semen Smears For Morphological Defects (%)mentioning

confidence: 99%

Specific sperm abnormalities observed in rams (Ovis aries) following cypermethrin treatment

Azubuike¹,

Ogwu²,

Ibrahim³

et al. 2017

J. Cell Anim. Biol.

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This work was designed to make further observations on the specific types of sperm abnormalities present in Cypermethrin treated rams and contribute information towards understanding how Cypermethrin affects male's reproduction. Sixteen sexually-mature, healthy Yankasa rams aged 18 to 30 months and weighing between 21.5 to 46.5 kg were used. The 16 rams were divided equally into two groups (A and B). Group A served as the treatment group, while B served as the control. The rams in Group A were given Cypermethrin (3%) at the dose rate of 3 mg/kg (0.1 ml/kg) body weight, topically as pour-on. The control rams were given distilled water at the same dose rate and route. These treatments were repeated every two weeks for a period of 12 weeks. Semen was collected on weekly basis from each ram during the experimental period by means of a hand held electro-ejaculator (EE). The morphological sperm abnormalities/defects were determined by examining semen smears stained with eosin-nigrosin on a glass slide. Results showed that sperm abnormalities recorded were detached head, mid piece droplet, free tail, coiled tail and bent tail. There was no significant difference in the percentage of these abnormalities recorded between the two groups (p>0.05) from week 1 to 12. It is concluded that Cypermethrin did not induce any specific sperm abnormalities in rams treated with 3 mg/kg body weight as pour-on. Sperm death in Cypermethrin label dose treated rams may be mediated through oxidative stress. It was recommended that more studies be directed towards stress biomarkers in Cypermethrin treated rams.

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Ameliorative effect of N‐acetylcysteine on the testicular tissue of adult male albino rats after glyphosate‐based herbicide exposure

Hashim

Bashir

Yasin

et al. 2022

J Biochem & Molecular Tox

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Glyphosate (GLP) is a broad-spectrum herbicide that is frequently used in crop production, but its residues remain in foodstuffs. This, in turn, has led to potential adverse effects on both human and animal health. Recent studies emphasized that GLP induces teratogenic effects and reproductive disorders, but its mechanism of toxicity is highly debated. N-acetylcysteine (NAC) is well known for its potent antioxidant capacity in addition to anti-inflammatory and cytoprotective properties.Therefore, our study aimed to investigate the reproductive toxicity of GLP in mature rats and evaluate the possible ameliorative effect of NAC against this toxicity. To this end, 30 adult male rats were assigned into three groups (10 rats per group) as follows: Group I, negative control; group II, GLP-exposed; 375 mg/kg GLP, orally; group III, NAC-cotreated, 160 mg/kg NAC 1 h before GLP, plus GLP, 375 mg/kg orally for 6 weeks. At the end of the experiment, the testicles were collected for semen analysis and biochemical, histopathological, and immunohistochemical studies. GLP-exposed rats exhibited disturbances in seminal parameters and a significant increase in malondialdehyde levels and expression of apoptotic markers. Several histopathological changes were observed, including strong immunoreactions for caspase-3 and proliferating cell nuclear antigen. Conversely, the administration of NAC before GLP was able to improve seminal parameters, attenuate the induced oxidative stress and apoptosis in addition to the regeneration of testicular damage.In conclusion, NAC can ameliorate the reproductive toxicity induced by GLP to an acceptable degree.

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Quality and reactive oxygen species of extended canine semen after vitamin C supplementation

Cited by 39 publications

References 38 publications

Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

Specific sperm abnormalities observed in rams (Ovis aries) following cypermethrin treatment

Ameliorative effect of N‐acetylcysteine on the testicular tissue of adult male albino rats after glyphosate‐based herbicide exposure

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