Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem

Bergstein-Galan, Tácia Gomes; Weiss, Romildo Romualdo; Bertol, Melina Andrea Formighieri; Abreu, Ana Claudia Machinski Rangel de; Busato, Eduarda Maciel; Kozicki, Luiz Ernandes; Bicudo, Sony Dimas

doi:10.1016/j.theriogenology.2017.04.001

Cited by 14 publications

(14 citation statements)

References 45 publications

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“…The uterine horns were visualized with a 5 mm and 30° angulated laparoscope (Karl Stroz, Tuttlingen, German). The spermatozoa were deposited into the uterine lumen in the middle of the uterine horn using an Robertson pipet (Minitube Brasil, Porto Alegre, Brazil) (Bergstein‐Galan, Weiss, Bertol, et al, ; Bergstein‐Galan, Weiss, Kozicki, & Bicudo, ).…”

Section: Methodsmentioning

confidence: 99%

Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep

Bergstein-Galan¹,

Weiss

Kozicki

2018

Reprod Domestic Animals

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Contents Multiple ovulation and embryo transfer (MOET) is an important tool in the sheep industry for increasing numbers of genetically superior individuals. The objective of this study was to evaluate the effect of semen source (frozen or fresh), the number of embryo collection procedures for each donor (NECP), the season in which embryo transfer and collection was performed, and the age and breed of the donor, on the number of recovered embryos and pregnancy rates after embryo transfer. The Alamos Genetics’ flushing station database was used. This consisted of 140 embryo collection procedures, from 53 Dorper and White Dorper sheep donors, aged between one and eight years, totalling 1,200 collected embryos. Neither the number of retrieved embryos nor the pregnancy rate was affected by the semen preservation method (fresh or frozen), NECP or the age and breed of donor. The season did not affect the number of collected embryos but had a significant effect (p < 0.05) on the recipient pregnancy rate, with higher pregnancy rates reported in the winter (65.57% ± 25.33%) compared with spring (37.11% ± 33.27%), summer (29.95% ± 28.33%) or autumn (35.03% ± 31.66%). There is an estimated increase of 98.4% and 71.5% of embryos recovered in the spring and summer seasons, respectively, when winter is used as reference. The survival of embryos is significantly higher when implanted during the breeding season, more specifically in winter. Embryo collection can be carried out throughout the year in sheep, but there may be a marginal advantage in the use of superovulation and fresh embryo transfer programmes in the autumn and winter.

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Section: Methodsmentioning

confidence: 99%

Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep

Bergstein-Galan¹,

Weiss

Kozicki

2018

Reprod Domestic Animals

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“…Bergstein‐Galan et al. () stored epididymides at room temperature (18–25°C); however, increases in sperm acrosome damage and viability were observed. Nichi et al.…”

Section: Discussionmentioning

confidence: 99%

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Angrimani

Nagai

Rui

et al. 2017

Reprod Domestic Animals

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Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post-mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer-assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3'3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.

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“…A cauda de epidídimo é fonte de espermatozoides viáveis que são capazes de fertilizar oócitos (LONE et al, 2011). A fertilização de oócitos, in vivo ou in vitro, por espermatozoides colhidos da cauda de epidídimo já foi relatada em ratos (KANEKO et al, 2009;SONGSASEN;TONG;LEIBO, 1998) equinos (MONTEIRO et al, 2011), bovinos (BERTOL et al, 2016MARTINS et al, 2009), ovinos (ABELLA et al, 2015ÁLVAREZ et al, 2012;BERGSTEIN-GALAN et al, 2017b;KAABI et al, 2003) (ÁLVAREZ et al, 2012;BERGSTEIN-GALAN et al, 2017b;KAABI et al, 2003).…”

Section: Fertilidade De Espermatozoides Epididimáriosunclassified

CARACTERIZAÇÃO SOCIODEMOGRÁFICA DOS PRODUTORES DE TILÁPIA DO NILO (Oreochromis niloticus) DO AÇUDE DO CASTANHÃO

Silva¹,

Silva²,

Pereira³

et al. 2019

Investigação Científica E Técnica Em Ciência Animal 2

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Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18–25 °C) for up to 48 h post mortem

Cited by 14 publications

References 45 publications

Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep

Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

CARACTERIZAÇÃO SOCIODEMOGRÁFICA DOS PRODUTORES DE TILÁPIA DO NILO (Oreochromis niloticus) DO AÇUDE DO CASTANHÃO

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