Quality analysis of Polygala tenuifolia root by ultrahigh performance liquid chromatography–tandem mass spectrometry and gas chromatography–mass spectrometry

Wu, Dan; He, Jirui; Jiang, Yueming; Yang, Bao

doi:10.1016/j.jfda.2014.07.009

Cited by 17 publications

(6 citation statements)

References 27 publications

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“…Figure 3 shows that TS mainly contains polygala saponins. According to the mass spectrometric data and reported literature [26, 27], 16 polygala saponins were characterized from TS (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption

Wang

Zhang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Oligosaccharide esters, which are among the main active components of Polygalae Radix (PR), demonstrate significant pharmacological activities in the human nervous system. In our previous research, some other constituents in PR were able to improve the bioavailability of oligosaccharide esters such as sibiricose A5 (SA5), sibiricose A6 (SA6), and 3,6′-disinapoyl sucrose (DISS), but the related components and their underlying mechanisms remain unknown. The present study aimed to investigate the intestinal absorptive profile of SA5, SA6, and DISS and the absorptive behavior influenced by the coadministration of polygalaxanthone III and total saponins of PR (TS) using an in vitro everted rat gut sac model, along with the possible mechanisms that may influence absorption. The results showed that TS could significantly enhance the absorption of SA5, SA6, and DISS monomers. Verapamil, a P-glycoprotein inhibitor, was able to elevate the absorption of SA5 and SA6, and an absorption experiment using Rho123 led us to conclude that TS influenced the absorption of SA5 and SA6 in a manner similar to that of a P-glycoprotein inhibitor. Sodium caprate, a paracellular absorption enhancer, was found to increase the absorption of SA5, SA6, and DISS. Results showed that the absorption mechanisms of SA5 and SA6 may combine active transport with paracellular passive penetration, while DISS’s absorption was dominated by paracellular passive penetration. However, the relationship between polygala saponins and the absorption of SA5, SA6, and DISS by paracellular passive penetration remain to be examined. This is the direction of our future research.

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Section: Resultsmentioning

confidence: 99%

Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption

Wang

Zhang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Although untargeted metabolomics for plant origin discrimination has been well established and thoroughly studied in previous studies [ 11 – 14 ], rice metabolomics, particularly the metabolite-based discrimination of white rice from different origins has received less attention. This fact comes from several reasons.…”

Section: Introductionmentioning

confidence: 99%

The integration of multi-platform MS-based metabolomics and multivariate analysis for the geographical origin discrimination of Oryza sativa L.

Lim

Lee

et al. 2018

Journal of Food and Drug Analysis

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For the authentication of white rice from different geographical origins, the selection of outstanding discrimination markers is essential. In this study, 80 commercial white rice samples were collected from local markets of Korea and China and discriminated by mass spectrometry-based untargeted metabolomics approaches. Additionally, the potential markers that belong to sugars & sugar alcohols, fatty acids, and phospholipids were examined using several multivariate analyses to measure their discrimination efficiencies. Unsupervised analyses, including principal component analysis and k-means clustering demonstrated the potential of the geographical classification of white rice between Korea and China by fatty acids and phospholipids. In addition, the accuracy, goodness-of-fit (R), goodness-of-prediction (Q), and permutation test p-value derived from phospholipid-based partial least squares-discriminant analysis were 1.000, 0.902, 0.870, and 0.001, respectively. Random Forests further consolidated the discrimination ability of phospholipids. Furthermore, an independent validation set containing 20 white rice samples also confirmed that phospholipids were the excellent discrimination markers for white rice between two countries. In conclusion, the proposed approach successfully highlighted phospholipids as the better discrimination markers than sugars & sugar alcohols and fatty acids in differentiating white rice between Korea and China.

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“…Until now, various analytical methods were established to perform the quantitative and qualitative appraisal of bioactive constituents in RP, such as high performance liquid chromatography (HPLC) [16,17,18], ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF) [19], and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [20,21,22], which contributed to RP’s quality evaluation. For our study, there were some limitations in methods that cannot meet our study, such as qualitative study performed only [19,22], quantitative research on limited compounds [17,18,20,21], and longer analytical time [16,18].…”

Section: Introductionmentioning

confidence: 99%

“…For our study, there were some limitations in methods that cannot meet our study, such as qualitative study performed only [19,22], quantitative research on limited compounds [17,18,20,21], and longer analytical time [16,18]. …”

Section: Introductionmentioning

confidence: 99%

Illumination on “Reserving Phloem and Discarding Xylem” and Quality Evaluation of Radix polygalae by Determining Oligosaccharide Esters, Saponins, and Xanthones

Yang

Chai

et al. 2018

Molecules

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The root of Polygala tenuifolia Willd. or Polygala sibirica L. exhibits protective effects on the central nervous system and is frequently used to treat insomnia, amnesia, and other cognitive dysfunction. In our study, we studied nine bioactive compounds spanning oligosaccharide esters, saponins, and xanthones by using a sensitive, efficient, and validated method established on ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry. The quantified result of interesting compounds proved that accumulation of those compounds were found in phloem rather than in xylem. By taking the standardized result of nine compound contents into account, the “Spider-web” analytical result of xylem and phloem from Radix polygalae (RP) unveiled the rationality of RP’s classical use in clinic including discarding the xylem and reserving the phloem. Moreover, the remarkable variation was also revealed from the quantitative result of 45 samples with different diameters from the different origins, which did not significantly correlate with the variation of RP’s diameter. Our study could shed the light on the quality assessment of RP for further research and illustrate the scientific connotation of the processing method of “discarding the xylem and reserving the phloem”.

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Quality analysis of Polygala tenuifolia root by ultrahigh performance liquid chromatography–tandem mass spectrometry and gas chromatography–mass spectrometry

Cited by 17 publications

References 27 publications

Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption

Intestinal Absorption Profile of Three Polygala Oligosaccharide Esters in Polygalae Radix and the Effects of Other Components in Polygalae Radix on Their Absorption

The integration of multi-platform MS-based metabolomics and multivariate analysis for the geographical origin discrimination of Oryza sativa L.

Illumination on “Reserving Phloem and Discarding Xylem” and Quality Evaluation of Radix polygalae by Determining Oligosaccharide Esters, Saponins, and Xanthones

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